Abstract
The nuclear gene mutantviridis-n 34 in barley has been characterized as a photosystem I mutant. The slow component of the light dependent absorption change at 518 nm and the photooxidation of cytochromef were greatly reduced in mutant leaves compared with wild-type leaves. The oxidation of cytochromef in mutant leaves irradiated with far-red light was only 7% of the value obtained with wild-type. The fast component of the 518 nm absorbance change was similar in wild-type and mutant leaves. The rate of photosystem I electron transport in the mutant is approximately 10% of the rate observed with wild-type thylakoids whereas photosystem II electron transport appears normal. Little or noP700 was detected in the mutant. The levels of cytochromes and ferredoxin-NADP+ oxidoreductase were normal. Mutant thylakoids were unable to generate a proton gradient upon illumination. Analysis of the polypeptide composition of thylakoids ofviridis-n 34 revealed that these are depleted in chlorophylla-protein 1 and three polypeptides believed to be iron-sulfur proteins. These four polypeptides are components of photosystem I particles isolated from wild-type barley.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- Asc:
-
ascorbic acid
- Chl:
-
chlorophyll
- Cyt:
-
cytochrome
- DBMIB:
-
dibromothymoquinone
- DCIP:
-
2,6-dichlorophenolindophenol
- DCMU:
-
3-(3,4-dichlorophenyl)-1,1-dimethylurea
- DTT:
-
dithiothreitol
- FeCN:
-
potassium ferricyanide
- GD:
-
Gramicidin D
- MeV:
-
methylviologen
- NADP+ :
-
β-nicotinamide adenine dinucleotide phosphate
- PD:
-
p-phenylenediamine
- PS:
-
photosystem
- SDS-PAGE:
-
sodium dodecyl sulfate polyacrylamide gel electrophoresis
- TMPD:
-
N,N,N′,N′-tetramethylphenylenediamine
- Tricine:
-
N-(tris-(hydroxymethyl)-methyl)glycine
- Tris:
-
tris-(hydroxymethyl)aminomethane
References
Arnon, D. I.: Copper enzymes in isolated chloroplasts. Polyphenoloxidase inBeta vulgaris. Plant Physiol. 24, 1–14 (1949)
Bendall, D. S., H. E. Davenport &R. Hill: Cytochrome components in chloroplasts of the higher plants. In: Methods Enzymol. (A. San Pietro, ed.). Academic Press, New York and London 23, 327–344 (1971)
Bengis, C. &N. Nelson: Purification and properties of the photosystem I reaction center from chloroplasts. J. Biol. Chem. 250, 2783–2788 (1975)
Bengis, C. &N. Nelson: Subunit structure of chloroplast photosystem I reaction center. J. Biol. Chem. 252, 4564–4569 (1977)
Bennoun, P., J. Girard &N.-H. Chua: A uniparental mutant ofChlamydomonas reinhardtii deficient in the chlorophyll-protein complex CPI. Molec. gen. Genet. 153, 343–348 (1977)
Boardman, N. K.: Comparative photosynthesis of sun and shade plants. Ann. Rev. Plant Physiol. 28, 355–377 (1977)
Buchanan, B. B. &D. I. Arnon: Ferredoxins from photosynthetic bacteria, algae, and higher plants. In: Methods Enzymol. (A. San Pietro, ed.) Academic Press, New York and London 23, 413–440 (1971)
Böhme, H., S. Reimer &A. Trebst: The effect of dibromothymoquinone, an antagonist of plastoquinone, on non-cyclic and cyclic electron flow systems in isolated chloroplasts. Z. Naturforsch. 26b, 341–352 (1971)
Chow, W. S. &A. B. Hope: Proton translocation, electron transport and photophosphorylation in isolated chloroplasts. Aust. J. Plant Physiol. 4, 647–665 (1977)
Chua, N.-H. &P. Bennoun: Thylakoid membrane polypeptides ofChlamydomonas reinhardtii: Wild type and mutant strains deficient in photosystem II reaction center. Proc. Nat. Acad. Sci. USA 72, 2175–2179 (1975)
Chua, N.-H. &N. W. Gillham: The sites of synthesis of the principal thylakoid membrane polypeptides inChlamydomonas reinhardtii. J. Cell Biol. 74, 441–452 (1977)
Chua, N.-H., K. Matlin &P. Bennoun: A chlorophyll-protein complex lacking in photosystem I mutants ofChlamydomonas reinhardtii: J. Cell Biol. 67, 361–377 (1975)
Fork, D. C. &U. W. Heber: Studies on electron-transport reactions of photosynthesis in plastome mutants of Oenothera. Plant Physiol. 43, 606–612 (1968)
Givan, A. L. &R. P. Levine: The photosynthetic electron transport chain of Chlamydomonas reinhardi. VII. Photosynthetic phosphorylation by a mutant strain of Chlamydomonas reinhardi deficient in active P700. Plant Physiol. 42, 1264–1268 (1967)
Henningsen, K. W. &N. K. Boardman: Development of photochemical activity and the appearance of the high potential form of cytochromeb-559 in greening barley seedlings. Plant Physiol. 51, 1117–1126 (1973)
Henningsen, K. W., N. C. Nielsen &R. M. Smillie: The effect of nuclear mutations on the assembly of photosynthetic membranes in barley. Port. Acta Biol. Ser. A 14, 323–344 (1974)
Herrmann, F.: Genetic control of pigment-protein complexes I and Ia of the plastid mutant en:alba-1 ofAntirrhinum majus. FEBS Lett. 19, 267–269 (1971)
Herrmann, F.: Struktur und Funktion der genetischen Information in den Plastiden. II. Untersuchung der photosynthesedefekten Plastommutantealba-1 vonAntirrhinum majus L. Photosynthetica 5, 258–266 (1971)
Herrmann, F., D. Matorin, K. Timofeev, T. Börner, A. B. Rubin &R. Hagemann: Structure and function of the genetic information in plastids. IX. Studies on primary reactions of photosynthesis in plastom mutants ofAntirrhinum majus andPelargonium zonale having impaired photosynthesis. Biochem. Physiol. Pflanzen 165, 393–400 (1974)
Herrmann, F. H., B. Schumann, T. Börner &R. Knoth: Structur und Funktion der genetischen Information in den Plastiden. XII. Die plastidalen Lamellarproteine der photosynthesedefekten Plastommutante en:gil-1 («Mrs. Pollock») und der Genmutante «Cloth of Gold» vonPelargonium zonale Ait. Photosynthetica 10, 164–171 (1976)
Hiyama, T. &B. Ke: Difference spectra and extinction coefficients of P700. Biochim. Biophys. Acta 267, 160–171 (1972)
Høyer-Hansen, G., B. L. Møller &L. C. Pan: Identification of coupling factor subunits in thylakoid polypeptide patterns of wild-type and mutant barley thylakoids using crossed immunoelectrophoresis. Carlsberg Res. Commun. 44, 337–351 (1979)
Høyer-Hansen, G. &D. J. Simpson: Changes in the polypeptide composition of internal membranes of barley plastids during greening. Carlsberg Res. Commun. 42, 379–389 (1977)
Izawa, S., T. N. Connolly, G. D. Winget &N. E. Good: Inhibition and uncoupling of photophosphorylation in chloroplasts. Brookhaven Symp. Biology 19, 169–187 (1967)
Katoh, S.: Plastocyanin. In: Methods Enzymol. (A. San Pietro, ed.) Academic Press, New York and London 23, 408–413 (1971)
Larkum, A. W. D. &W. D. Bonner: Light-induced absorbance changes of P518 in intact plastids. Biochim. Biophys. Acta 256, 396–408 (1972)
Lowry, O. H., N. J. Rosebrough, A. L. Farr &R. J. Randall: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)
Machold, O. &G. Høyer-Hansen: Polypeptide composition of thylakoids from viridis and xantha mutants in barley. Carlsberg Res. Commun. 41, 359–366 (1976)
Machold, O., D. J. Simpson &B. L. Møller: Chlorophyll-proteins of thylakoids from wild-type and mutants of barley (Hordeum vulgare L.). Carlsberg Res. Commun. 44, 235–254 (1979)
Miles, D.: Mutants of higher plants: Maize. In: Methods Enzymol. (A. San Pietro, ed.) Academic Press, New York and London 69, 3–23 (1979)
Miles, C. D., J. P. Markwell &J. P. Thornber: The effect of nuclear mutation in maize on photosynthetic activity and content of chlorophyll-protein complexes. Plant Physiol. 64, 690–694 (1979)
Nielsen, N. C. &R. M. Smillie: The effect ofp-phenylenediamine and dibromothymoquinone on the photosynthetic electron transport and proton pump activities of isolated barley chloroplasts. Arch. Biochem. Biophys. 186, 52–59 (1978)
Nielsen, N. C., R. M. Smillie, K. W. Henningsen, D. von Wettstein &C. S. French: Composition and function of thylakoid membranes from grana-rich and grana-deficient chloroplast mutants of barley. Plant Physiol. 63, 174–182 (1979)
Rühle, W. &A. Wild: Measurements of cytochrome f and P-700 in intact leaves ofSinapis alba grown under high-light and low-light conditions. Planta 146, 377–385 (1979)
Sauer, K., P. Mathis, S. Acker &J. A. van Best: Electron acceptors associated withP-700 in triton solubilized photosystem I particles from spinach chloroplasts. Biochim. Biophys. Acta 503, 120–134 (1978)
Simpson, D., G. Høyer-Hansen, N.-H. Chua &D. von Wettstein: The use of gene mutants in barley to correlate thylakoid polypeptide composition with the structure of the photosynthetic membrane. Proc. Int. Congr. Photosynth., 4th (D. O. Hall, J. Coombs & T. W. Goodwin, eds.) Biochemical Society, England pp. 537–548 (1977)
Simpson, D., B. L. Møller &G. Høyer-Hansen: Freeze-fracture structure and polypeptide composition of thylakoids of wild-type and mutant barley plastids. In: Chloroplast Development (G. Akoyunoglou & J. H. Argyroudi-Akoyunoglou, eds.) Elsevier/North-Holland, Biomedical Press, Amsterdam pp. 507–512 (1978)
Smillie, R. M., N. C. Niflsen, K. W. Henningsen &D. von Wettstein: Development of photochemical activity in chloroplast membranes. I. Studies with mutants of barley grown under a single environment. Aust. J. Plant Physiol. 4, 415–438 (1977)
Smillie, R. M. &R. Nott: Assay of chilling injury in wild and domestic tomatoes based on photosystem activity of the chilled leaves. Plant Physiol. 63, 796–801 (1979)
Telfer, A., J. Barber, P. Heathcote &M. C. W. Evans: Variable chlorophylla fluorescence fromP-700 enriched photosystem I particles dependent on the redox state of the reaction centre. Biochim. Biophys. Acta 504, 153–164 (1978)
Thorne, S. W., G. Horvath, A. Kahn &N. K. Boardman: Light-dependent absorption and selective scattering changes at 518 nm in chloroplast thylakoid membranes. Proc. Nat. Acad. Sci. USA 72, 3858–3862 (1975)
Trebst, A.: On the role of plastoquinone and on the nature of the primary acceptor of photosystem I in photosynthetic electron transport of chloroplasts. In: Proc. Int. Congr. Photosynth. Res., 2nd. The Hague, Neth. 1, 399–417 (1971)
Trebst, A.: Measurement of Hill reactions and photoreduction. In: Methods Enzymol. (A. San Pietro, ed.) Academic Press, New York and London 24, 146–164 (1972)
Trebst, A.: Energy conservation in photosynthetic electron transport of chloroplasts. Ann. Rev. Plant. Physiol. 25, 423–458 (1974)
Trebst, A., E. Harth &W. Draber. On a new inhibitor of photosynthetic electron-transport in isolated chloroplasts. Z. Naturforsch. 25b, 1157–1159 (1970)
Trebst, A. &S. Reimer: Energy conservation in photoreductions by photosystem II. Reversal of dibromothymoquinone inhibition of Hill reactions of phenylenediamines. Z. Naturforsch. 28c 710–716 (1973)
Wettstein D. von &K. Kristiansen: Stock list for nuclear gene mutants affecting the chloroplast. Barley Genet. Newslett. 3, 113–117 (1973)
Wettstein, D. von, B. L. Møller, G. Høyer-Hansen & D. Simpson: Genetic manipulation of photosystem I and II in chloroplast membranes. In: Proceedings 2nd. Coordination Meeting of Contractors EC Solar Energy R & D Programme, Commission of the European Communities. No. 3560 EN, III/9, pp. 1–9 (1978)
Wettstein, D. von, B. L. Møller, G. Høyer-Hansen &D. Simpson: Mutants in the analysis of the photosynthetic membrane polypeptides. In: Origin of chloroplasts. (J. A. Schiff & R. Y. Stanier, eds.) Elsevier/North-Holland, Biomedical Press, Amsterdam. in press (1980)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Møller, B.L., Smillie, R.M. & Høyer-Hansen, G. A photosystem I mutant in barley (Hordeum vulgare L.). Carlsberg Res. Commun. 45, 87–99 (1980). https://doi.org/10.1007/BF02906510
Issue Date:
DOI: https://doi.org/10.1007/BF02906510