Abstract
Mass spectra of trimethylsilyl derivatives of the following compounds, which are related to the biosynthesis of the cyanogenic glucoside dhurrin, are described: tyrosine,N-hydroxytyrosine,p-hydroxyphenylacetaldoxime,p-hydroxyphenylacetonitrile,p-hydroxybenzaldehyde, tyramine,p-hydroxyphenylpyruvic acid oxime, andp-hydroxyphenylpyruvic acid. Accurate mass measurements and metastable transitions support the proposed fragmentation mechanisms. Based on the mass spectrometry data presented, a method is described by which compounds possibly involved as intermediates in the biosynthesis of cyanogenic glucosides can be identified in biosynthetic reaction mixtures.
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Abbreviations
- GLC-MS:
-
gas liquid chromatography-mass spectrometry
- TLC:
-
thin layer chromatography
- TMS:
-
trimethylsilyl
References
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Møller, B.L., Conn, E.E. & Sweeley, C.C. Mass spectrometric identification of intermediates in the biosynthesis of cyanogenic glucosides. Carlsberg Res. Commun. 44, 367–379 (1979). https://doi.org/10.1007/BF02906186
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DOI: https://doi.org/10.1007/BF02906186