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Electrochromatography of amino acid mixtures in fermentation liquors

ЭлектрохроматограФия смеси аминокислот в Ферментационных жидкостях

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Abstract

The substitution of the phenolic chromatography dimension, by electrophoresis at 500 to 1000 volts, in a veronal buffer, pH 8.6, μ 0.02 followed by triple rechromatography in a completely miscible butanol, acetic acid, water solvent system, leads to clear, easily reproducible electrochromatography patterns. A reliable economical chromatography cabinet is described. The technique allows the analysis of many samples conveniently, and some important characteristics of unidentifiable components can be readily predicted.

Abstract

Применение электрофореза при 500–1000 вольт в вероналовом буферном растворе при pH 8,6, μ 0,02 вместо проявления в феноле и последующая тройная рехроматография во вполне смешивающейся системе бутанол—уксусная кислота—вода (4∶1∶1) дают отчетливые и легко воспроизводимые результаты. Описано экономное хроматографическое устройство. Метод делает возможным надежный анализ большого количества образцов, причем нетрудно предсказать некоторые важные характеристики неидентифицированных веществ.

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Author notes

  1. M. K. El-Marsafy

    Present address: El-Nasr Pharmaceutical Chemicals Co., Abouzabal, Cairo, Egypt, UAR

  2. Abdel-Gawad Zenab

    Present address: Research Department, Production Laboratories, Ministry of Public Health, Agouza, Cairo, Egypt, UAR

Authors and Affiliations

  1. Department of Antibiotics and Experimental Mycology, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague 6

    M. K. El-Marsafy, Z. Řeháček & Abdel-Gawad Zenab

Authors
  1. M. K. El-Marsafy
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  2. Z. Řeháček
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  3. Abdel-Gawad Zenab
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El-Marsafy, M.K., Řeháček, Z. & Zenab, AG. Electrochromatography of amino acid mixtures in fermentation liquors. Folia Microbiol 8, 362–369 (1963). https://doi.org/10.1007/BF02906034

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  • DOI: https://doi.org/10.1007/BF02906034

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