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A new method to localize acid phosphatase using the confocal laser-scanning microscope

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Pathology & Oncology Research

Abstract

The aim of the study was to develop a technique for the detection of acid phosphatase enzyme activity by confocal laser-scanning microscope using the histochemical acid phosphatase detection method (after Barka and Anderson 1962, modified by Bowen and Lewis 1985) routinely used for light microscopy. The density and the distribution of enzyme reaction product is dependent on the incubation time, as shown by different confocal images or ELISA reader. The inhibition of the enzyme activity with metal ions shows the same profile known from the literature. This staining method seems to be useful to demonstrate subcellular distribution of the enzyme in the lysosomes and in the Golgi apparatus.

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Authors and Affiliations

  1. 1st Institute of Pathology and Experimental Cancer Research, Department of Molecular Pathology, Joint Research Organization of the Hungarian Academy of Sciences and Semmelweis University of Medicine, Budapest, Hungary

    Zsolt Szegedi & Béla Szende

  2. Department of Pure and Applied Biology, University of Cardiff, Cardiff, Wales, UK

    Farhana Amin & Ivor Delme Bowen

  3. Üllői út 26., H-1085, Budapest, Hungary

    Zsolt Szegedi

Authors
  1. Zsolt Szegedi
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  2. Farhana Amin
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  3. Béla Szende
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  4. Ivor Delme Bowen
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Correspondence to Zsolt Szegedi.

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Szegedi, Z., Amin, F., Szende, B. et al. A new method to localize acid phosphatase using the confocal laser-scanning microscope. Pathol. Oncol. Res. 4, 217–223 (1998). https://doi.org/10.1007/BF02905252

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  • DOI: https://doi.org/10.1007/BF02905252

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