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Examination of Galectin Localization Using Confocal Microscopy

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Galectins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1207))

Abstract

Confocal microscopy provides a unique modality to examine the expression and localization of biomolecules in a variety of settings. Using this technique, an image is acquired from the focal plane of the objective using focused laser light, making it possible to work within the resolution limit of the optical system. In addition, by acquiring multiple images from a variety of focal planes, stacked series of images can provide clear spatial localization of a probed structure or protein. We describe herein the immunofluorescence methods for galectin staining in frozen sections of tissue for galectin localization using confocal microscopy.

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Acknowledgments

We thank the technicians Maria Vani Alves (Department of Cell Biology and Pathogenic agents from Faculty of Medicine of Ribeirão Preto-University of São Paulo) and Márcia Sirlene Zardin Graeff (Research Center-Faculty of Odontology of Bauru-University of São Paulo) for their invaluable assistance.

The opinions or assertions contained herein are the private views of the authors and are not to be construed as official or as reflecting the views of our department.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences of Ribeirão Preto, Department of Clinical, Toxicological and Bromatological Analysis, University of Sao Paulo, Ribeirão Preto-S, Brazil

    Daniel Giuliano Cerri, Lílian Cataldi Rodrigues, Marise Lopes Fermino & Marcelo Dias Baruffi

  2. Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA, USA

    Connie M. Arthur & Sean R. Stowell

  3. Institute of Biological and Natural Sciences, Triângulo Mineiro Federal University, Uberaba, MG, Brazil

    Lenaldo Branco Rocha

Authors
  1. Daniel Giuliano Cerri
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  2. Connie M. Arthur
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  3. Lílian Cataldi Rodrigues
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  4. Marise Lopes Fermino
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  5. Lenaldo Branco Rocha
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  6. Sean R. Stowell
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  7. Marcelo Dias Baruffi
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Corresponding author

Correspondence to Marcelo Dias Baruffi .

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Editors and Affiliations

  1. Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA

    Sean R. Stowell

  2. Department of Biochemistry, Emory University School of Medicine, Atlanta, GA, Georgia, USA

    Richard D. Cummings

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Cerri, D.G. et al. (2015). Examination of Galectin Localization Using Confocal Microscopy. In: Stowell, S., Cummings, R. (eds) Galectins. Methods in Molecular Biology, vol 1207. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1396-1_23

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  • DOI: https://doi.org/10.1007/978-1-4939-1396-1_23

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1395-4

  • Online ISBN: 978-1-4939-1396-1

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