Summary
Normal and pathological lymphoid cells, collected from different sources, were cryopreserved using a programmed freezing procedure. With this cryopreservation technique, the percentage of T and B cell surface markers and the proliferative response to mitogens were not influenced by 21 and 35 days of storage in liquid nitrogen. The recovery percentage of the lymphoid cells was satisfactory when fetal calf serum was added, as a protein source, to the medium during freezing and thawing phases, while a very low percentage of cells was recovered if the fetal calf serum was omitted.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Boyum A.: Separation of leucocytes from blood and bone marrow.—Scand. J. clin. Lab. Invest.21 (Suppl. 97), 77, 1968.
Du Bois M. J. G. J., Schellekens P. Th. A., De Wit J. J. F. M., Eijsvoogel V. P.:In vitro reactivity of human lymphocytes after cryopreservation using a programmed cooling device.— Scand. J. Immunol.5 (Suppl. 5), 17, 1976.
Factor M. A., Strong D. M., Miller J. L., Sell K. W.: Cell-mediated lymphocytotoxicity followingin vitro sensitization of frozen stored human mononuclear cells in heterologous serum.—Cryobiology12, 521, 1975.
Pernis B., Forni L., Amante L.: Immunoglobulin spots on the surface of rabbit lymphocytes— J. exp. Med.132, 1001, 1970.
Schaefer U. W., Dicke K. A., Van Bekkun D. W.: Recovery of hematopoiesis in lethally irradiated monkeys by frozen allogenic bone marrow grafts.—Europ. J. clin. biol. Res.17, 483, 1972.
Special Technical Report: Identification, enumeration and isolation of B and T lymphocytes from human peripheral blood—Scand. J. Immunol.3, 521, 1974.
Thierry C., Turc J. M., Valles H., Serrcu B.: Rcutine technique of lymphocyte cryopreservation evaluated byin vitro tests of immune response—Europ. J. Cancer13, 367, 1977.
Thorpe P. E., Knight S. C., Farrant J.: Optimal conditions for the preservation of mouse lymph node cells in liquid nitrogen using cooling rate techniques—Cryobiology13, 126, 1976.
Author information
Authors and Affiliations
Additional information
Supported by grant no. 7701469 from theConsiglio Nazionale delle Ricerche (CNR), Rome, Italy andStiftung Volkswagenwerk, Bundesrepublik Deutschland.
Rights and permissions
About this article
Cite this article
Delfini, C., Grilli, G., Polchi, P. et al. Cryopreservation of human lymphoid cells from various tissues. La Ricerca Clin. Lab. 9, 61–66 (1979). https://doi.org/10.1007/BF02905028
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02905028