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Isolation and Cryopreservation of Mononuclear Cells from Peripheral Blood and Bone Marrow of Blood Cancer Patients

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Cancer Cell Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2645))

Abstract

Peripheral blood and bone marrow aspirates are routinely obtained from blood cancer patients for diagnostic investigations and provide an accessible source of patient-specific cancer cells, as well as non-malignant cells, for research proposes. The simple and reproducible method presented here allows isolation of viable mononuclear cells, including malignant cells, from fresh peripheral blood or bone marrow aspirates using density gradient centrifugation. The cells obtained using the protocol described can be further purified for a variety of cellular, immunological, molecular, and functional assays. In addition, these cells can be cryopreserved and bio-banked for future research studies.

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Acknowledgments

This work was funded by Science Foundation Ireland, Irish Blood Transfusion Service, and the Bone Marrow for Leukaemia Trust.

Author information

Authors and Affiliations

  1. The John Durkan Leukaemia Laboratory, Trinity Translational Medicine Institute, Trinity College and St James’s Hospital, Dublin, Ireland

    Sarah Brophy, Rebecca Amet & Anthony M. McElligott

  2. Cryobiology Laboratory Stem Cell Facility, St James’s Hospital, Dublin, Ireland

    Hayley Foy-Stones & Nicola Gardiner

  3. Trinity St. James’s Cancer Institute, Trinity College and St James’s Hospital, Dublin, Ireland

    Anthony M. McElligott

Authors
  1. Sarah Brophy
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  2. Rebecca Amet
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  3. Hayley Foy-Stones
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  4. Nicola Gardiner
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  5. Anthony M. McElligott
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Corresponding author

Correspondence to Anthony M. McElligott .

Editor information

Editors and Affiliations

  1. Trinity Translational Medicine Institute, Trinity Centre for Health Sciences, Dublin, Ireland

    Dania Movia

  2. Trinity Translational Medicine Institute, Trinity Centre for Health Sciences, Dublin, Ireland

    Adriele Prina-Mello

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Brophy, S., Amet, R., Foy-Stones, H., Gardiner, N., McElligott, A.M. (2023). Isolation and Cryopreservation of Mononuclear Cells from Peripheral Blood and Bone Marrow of Blood Cancer Patients. In: Movia, D., Prina-Mello, A. (eds) Cancer Cell Culture. Methods in Molecular Biology, vol 2645. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3056-3_10

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  • DOI: https://doi.org/10.1007/978-1-0716-3056-3_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3055-6

  • Online ISBN: 978-1-0716-3056-3

  • eBook Packages: Springer Protocols

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