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PCNA positivity in the telencephalic matrix areas in the adult of a Teleost,Carassius carassius L

PCNA positività nelle aree matrici telencefaliche nell’adulto di un Teleosteo,Carassius carassius L

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Abstract

In normal adult Teleostei, classical histology and autoradiographic techniques have been used to identify the telencephalic hemispheres on the dorso-lateral (zona germinativa dorsalis) and medial (zona germinativa ventralis) surfaces in the ependymal and/or peri-ependymal zone as elements capable of proliferating. In inequivocally adultCarassius carassius specimens we revisited these «matrix areas» using a recent proliferative marker that detects the expression of the Proliferating Cellular Nuclear Antigen (PCNA), a reliable immunocytochemical test of the essential role played by PCNA in the cell cycle, its ubiquity and the theoretical bases on which this method rested. It was found that in the telencephalon the distribution of the DNA synthesis activity is not continuous and uniform in an antero-posterior direction. A non negligible PCNA positivity is actually detectable at the anterior pole of the hemispheres and above all posteriorly, at the level of thezona germinativa ventralis while undifferentiated elements are visible in thezona germinativa dorsalis, in very small numbers in the lateral portion and sporadically in the upper section. Overall it would seem that in theCarassius telencephalon spontaneous neurogenic potential has now become residual. These findings are discussed in the light of literature data and above all of the age of our specimens.

Riassunto

Nei Teleostei adulti normali, l’istologia classica e le tecniche autoradiografiche, avevano identificato negli emisferi telencefalici sulle superfici dorso-laterale (zona germinativa dorsalis) e mediale (zona germinativa ventralis), in sede ependimale e/o peri-ependimale, elementi in grado di proliferare. InCarassius carassius inequivocabilmente adulti abbiamo rivisitato tali «aree matrici», utilizzando un recentemarker proliferativo che rivela l’espressione dellaProliferating Cellular Nuclear Antigen (PCNA), test immunocitochimico affidabile per il ruolo essenziale svolto dalla PCNA nel ciclo cellulare, la sua ubiquitarietà e le basi teoriche su cui poggia tale metodica. Si è rilevato che nel telencefalo la distribuzione dell’attività di sintesi del DNA non è, in senso antero-posteriore, continua ed uniforme. Infatti, una non trascurabile PCNA positività è evidenziabile al polo anteriore degli emisferi, specie posteriormente a livello dellazona germinativa ventralis, mentre elementi indifferenziati si osservano nellazona germinativa dorsalis con molta parsimonia nella porzione laterale ed episodicamente nel tratto superiore. In una visione d’insieme sembrerebbe che nel telencefalo delCarassius le potenzialità neurogeniche spontanee siano ormai residuali. Tali rilevanze vengono discusse alla luce dei dati forniti dalla bibliografia e soprattutto dell’età dei nostri esemplari.

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Authors and Affiliations

  1. Dipartimento di Biologia animale e dell’Uomo (Sede di Anatomia comparata), Università degli Studi di Roma «La Sapienza», Via A. Borelli, 50, 00161, Roma

    Vito Margotta, Antonio Morelli, Enrico Gelosi & Laura Alfei

  2. Agenzia Regionale per lo Sviluppo e l’Innovazione dell’Agricoltura del Lazio (A.R.S.I.A.L.), Stabilimento Ittiogenico di Roma, Via della Stazione Tiburtina, 11, 00162, Roma

    Vito Margotta, Antonio Morelli, Enrico Gelosi & Laura Alfei

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  1. Vito Margotta
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Nella seduta del 20 aprile 2001.

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Margotta, V., Morelli, A., Gelosi, E. et al. PCNA positivity in the telencephalic matrix areas in the adult of a Teleost,Carassius carassius L. Rend. Fis. Acc. Lincei 12, 105–115 (2001). https://doi.org/10.1007/BF02904674

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