Skip to main content
SpringerLink
Log in

Covalent immobilization of oligoDNA on the surface of magnetic nanoparticles and surface-enhanced Raman scattering study

  • Reports
  • Published:
Chinese Science Bulletin

Abstract

The DNA magnetic nanoparticles are potentially useful in isolating and purifying DNA or RNA, directing-target-medicines, the development of DNA biosensors and biochips. Surface functionalized magnetic nanoparticles with monodispersed shape and size were prepared by coating nano-sized γ-Fe2O3 with silica in reverse microemulsion, and then thiol-compounds were immobilized onto the magnetic nanoparticles. After immobilizing oligoDNA modified with thiol-disulfide on the surface of the fictionalized magnetic nanoparticles, we obtained DNA-magnetic nanoparticles. The efficiency of the single-linking probes loading at the surfaces of magnetic nanoparticles was examined via hybridization experiment. Surface-enhanced Raman scattering methods were also effectively applied to observing the immobilization and hybridization processes mentioned above. The results demonstrated oligoDNA being availably connected to the surface of the magnetic nanoparticles.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Tan, W. H., Hilliard, L. R., Zhao, X. J., Immobilization of oligonucleotides onto silica nanoparticals for DNA hybridization studies, Analytica Chimica Acta, 2002, 470: 51–56.

    Article  Google Scholar 

  2. Bose, A., Srinivas, V. S., DNA isolation using avidin-coated magnetic nanoclusters, Colloids and Surfaces B: Biointerfaces, 1997, 8: 199–204.

    Article  Google Scholar 

  3. Nalwa, H. S., Handbook of Nanostructured Materials and Banotechnology, Vol. IV, San Diego, CA, Academic Press, 1999, pp. ix-x.

    Google Scholar 

  4. Ke, M. W., He, X. X., Tan, W. H. et al., Concentration of trace amounts oligonucleotide using super-paramagnetic DNA nanoenricher, Chemical Journal of Chinese Universities (in Chinese), 2003, 24(1): 40–42.

    Google Scholar 

  5. Chrisey, A., Lee, G. U. et al., Covalent attachment of synthetic DNA to self-assembled monolayer films, Nucleic Acids Research, 1996, 24: 3031–3039.

    Article  PubMed  CAS  Google Scholar 

  6. Day, J. R., Flora, P. S., John, E. F. et al., Immobilization of polynucleotides on magnetic particles, Biochem. J., 1991, 278: 735–740.

    PubMed  CAS  Google Scholar 

  7. Anderson, S., Roger, Y. H., Huang, Z. J. et al., Immobilization of oligonucleotides onto a glass support via disulfide bonds: a method for preparation, Analytical Biochemistry, 1999, 266: 23–30.

    Article  PubMed  Google Scholar 

  8. Tan, W. H., Santra, S., Tapec, R. et al., Synthesis and characterization of silica-coated iron oxide nanoparticles in microemusion: the effect of nonionic surfactants, Langmuir, 2001, 17: 2900–2906.

    Article  Google Scholar 

  9. Albert, P. P., Michel, P. B., Chellapah, P., Magnetic silica dispersions: preparation and stability of surface-modified silica particles with a magnetic core, Langmuir, 1994, 10: 92–99.

    Article  Google Scholar 

  10. Greenwood, N. N., Gibb, T. C., Mössbauer Spectroscopy, London: Champman and Hall Ltd., 1971.

    Google Scholar 

  11. Jiang, J. S., Gao, L., Yang, X. L. et al., Preparation and Mössbauer Studies of α-Fe2O3 nanoparticle, Acta Phys. Chin. Sin. (in Chinese), 2000, 16(4): 312–316.

    CAS  Google Scholar 

  12. Portier, J., Mornet, S., Vekris, A. et al., DNA-magnetite nanocomposite materials, Materials Letters, 2000, 42: 183–188.

    Article  Google Scholar 

  13. Grebel, H., Iqbal, Z., Lan, A., Detecting single-wall carbon nanotubes with surface-enhanced Raman scattering from metal coated periodic structures, Chemical Physics Letters, 2001, 348: 203–208.

    Article  CAS  Google Scholar 

  14. Maurizio Muniz-Miranda, Silver-doped silica colloidal nanoparticles: Characterization and optical measurements, Colloids and Surfaces A: Physicochem. Eng. Aspects, 2003, 217: 185–189.

    Article  CAS  Google Scholar 

  15. Rodriguez, R., Hernandez, G., Adsorption properties of silica sols modified with thiol groups, Journal of Non-Crystalline, Solids, 1999, 246: 209–215.

    Article  Google Scholar 

  16. Vlckova, B., Michl, M., Mojzes, P., Ag colloid-ethanethiol films: spacer-modified substrates for surface-enhanced resonance Raman scattering spectroscopy of chromophoric molecules, Vibrational Spectroscopy, 1999, 19: 239–242.

    Article  Google Scholar 

  17. Tuan, Vo-Dinh, Leonardo, R. Allain, Surface-enhanced Raman scattering detection of the breast cancer susceptibility gene BRCA1 using a silver-coated microarray platform, Analytica Chimica Acta, 2002, 469: 149–154.

    Article  Google Scholar 

  18. Zhu, Z. Y., Gu, R. A., Lu, T. H., The Applications of Raman Spectrum in Chemistry (in Chinese), Changchun: Northeast University Press. 1998.

    Google Scholar 

  19. Meng, L. Z., He, R. B., The Analysis of Organic Wavespectrum, Wuhan: Wuhan University Press, 1997.

    Google Scholar 

  20. Pan, J. L., The Applications of Exciting Raman Spectrum in Organic Chemistry (in Chinese), Beijing: Chemical Industry Press, 1986.

    Google Scholar 

  21. Yoshikawa, Iwagami, K., Morita, N. et al., Characterization of fluorine-doped silicon dioxide film by Raman spectroscopy, Thin Solid Films, 1997, 310: 167–170.

    Article  CAS  Google Scholar 

  22. Shen, H. B., Xia, J. F., Yang, H. F., Study onin situ surface enhanced Raman scattering (SERS) of 5′-AMP, 3t’-AMP and CAMP hydrolysis by Ce4+ ion, Acta Chmica Sinica (in Chinese), 2001, 59: 742–745.

    CAS  Google Scholar 

  23. Shen, H. B., Xia, J. F., Yang, H. F., Surface enhanced Raman scattering (SERS) spectra of AMP and DNA in sliver sol., Spectroscopy and Spectral Analysis (in Chinese), 2001, 21: 798–800.

    PubMed  CAS  Google Scholar 

  24. Shen, H. B., Zhou, W. J., Yang, H. F., Change of adsorption state of oligodeoxynucleotides on the sliver electrode surface with change of potential, Acta Phys. Chin. Sin. (in Chinese), 2002, 18: 490–494.

    CAS  Google Scholar 

  25. Pang, D. W., Zhao, Y. D., Hu Shen et al., DNA-modified electrodes Part 3: spectroscopic characterization of DNA-modified gold electrodes, Analytica Chimica Acta, 1999, 388: 93–101.

    Article  Google Scholar 

  26. Ke, W. H., Yu, D. W., Wu, J. Z., Raman spectroscopic study of the influence on herring sperm DNA of heat treatment and ultraviolet radiation, Spectrochimica Acta, Part A, 1999, 55: 1081–1090.

    Article  Google Scholar 

  27. Lu, D. S., Zhao, X. J., Jiang, S. et al., Interaction between intercalation type anticancer drugs and DNA studied by ultraviolet resonance Raman spectroscopy, Scienc in China, Series B, 1995, 38: 555–563.

    Google Scholar 

  28. Shen, H. B., Xia, J. F., Yang, H. F. et al., Hydrolysis of oligodeoxynucleotide phosphodiester linkages, Science in China, Series B, 2001, 44(2): 169–174.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry, Shanghai Normal University, 200234, Shanghai, China

    Hebai Shen, Youbao Wang & Haifeng Yang

  2. State Key Laboratory of Chemical & Biological Sensor and Metric, Hunan University, 410082, Changsha, China

    Haifeng Yang

  3. Department of Physics, Center of Functional Nanomaterials and Devices, East China Normal University, 200062, Shanghai, China

    Jisen Jiang

Authors
  1. Hebai Shen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Youbao Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Haifeng Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jisen Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hebai Shen.

About this article

Cite this article

Shen, H., Wang, Y., Yang, H. et al. Covalent immobilization of oligoDNA on the surface of magnetic nanoparticles and surface-enhanced Raman scattering study. Chin. Sci. Bull. 48, 2698–2702 (2003). https://doi.org/10.1007/BF02901759

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02901759

Keywords

Search

Navigation