Abstract
Based on the published sequence of profilin2 promoter ofArabidopsis thaliana, a full-length promoter (1667 bp) was amplified by PCR. The 5′-end deletion fragments with length of 1380, 1153, 969 and 597 bp were then fused withgus (uidA) gene respectively. Constructed plant expression vectors were individually transferred intoKalanchoe laciniata and transgenic plants regenerated. GUS histochemical assay confirmed that the full-length promoter Pfn1.7 was vascular-specific. Deletion assays showed that profilin2 promoter could be divided into three parts. Deletion of fragment 1 (−1667—−1380 bp) resulted in constitutive expression, suggesting that element(s) responsible for vascular-specific expression might exist in this region. Fragment 2 located at −1153—−597 bp strongly inhibitedgus gene expression. Fragment 3 (−597—−1 bp) is considered as a basic domain of profilin2.
Similar content being viewed by others
References
Datla, R., Anderson, J. W., Selvaraj, G., Plant promoters for transgene expression, Biotech. Ann. Rev., 1997, 3: 269.
Christensen, H. E. M., Ramachandran, S., Tan, C. T. et al.,Arabidopsis profilins are functionally similar to yeast profilins: Identification of a vascular bundle-specific profilin and a pollen-specific profilin, Plant J., 1996, 10 (2): 269.
Sambrook, J., Fritsch, E. F., Miniatis, T., Molecular Cloning, A Laboratory Manual, 2nd ed., New York: Cold Spring Harbor Laboratory Press, 1989.
Jia, S. R., Transformation inKalanchoe, in Plant Protoplasts and Genetic Engineering III, Biotechnology in Agriculture and Forestry (ed. Bajaj, Y. P. S.), Vol. 22, Berlin Heidelberg: Springer-Verlag, 1993, 234.
Jia, S. R., Yang, M. Z., Ott, R. et al., High frequency transformationof Kalanchoe laciniata, Plant Cell Rep., 1989, 8: 336.
Murashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant., 1962, 15: 473.
Zhu, Y. X., Li, Y., Modern Molecular Biology (in Chinese), Beijing: Higher-Education Press, 1997, 183.
Keller, B., Baumgartner, C., Vascular-specific expression of the bean GRP1.8 gene is negatively regulated, Plant Cell, 1991, 3: 1051.
Torres-Schumann, S., Ringli, C., Heierli, D. et al.,In vitro binding of the tomato bZIP transcriptional activator VSF-1 to a regulatory element that controls xylem-specific gene expression, The Plant J., 1996, 9 (3): 283.
Hatton, D., Sablowski, R., Yung, M. H. et al., Two classes of cis sequences contribute to tissue-specific expression of aPAL2 promoter in transgenic tobacco, The Plant J., 1995, 7(6): 859.
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Liu, Y., Wang, Z. & Jia, S. Effect of 5′-deletion ofArabidopsis profilin2 promoter on its vascular specific expression. Chin.Sci.Bull. 46, 1627–1630 (2001). https://doi.org/10.1007/BF02900622
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02900622