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Citric acid production byAspergillus niger using media containing low concentrations of glucose or corn starch

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Abstract

By using an appropriate ratio of carbon source to mineral components, we obtained comparable citrate yields in media containing different concentrations of glucose. The enzyme system of inoculum passed on gradually from “growth” state to “production” state during the mould growth. In the starch medium, the critical factors of citric acid production are the aeration efficiency of the medium and the amylase formation of the strain. The air interruption exhibited a prolonged inhibition of the production rate but not of the citrate yield in glucose medium while those parameters in starch medium containing excessive urea were briefly but severely inhibited. After being affected by these unfavorable conditions, the production activity ofAspergillus niger could be restored by applying an appropriate fermentation process.

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References

  • Bernfeld P.: Amylase α and β.Methods Enzymol. 1, 149–158 (1955).

    Article  CAS  Google Scholar 

  • Chung B.H., Chang H.N.: Aerobic fungal cells immobilized in a dual hollow-fiber bioreactor: Continuous production of citric acid.Biotechnol. Bioeng. 32, 205–212 (1988).

    Article  CAS  PubMed  Google Scholar 

  • Clark P.S., Ito K., Horitsu H.: Effect of manganese and other heavy metals on submerged citric acid fermentation of molasses.Biotechnol. Bioeng. 8, 465–471 (1966).

    Article  CAS  Google Scholar 

  • Dawson M.W., Maddox I.S., Boag I.F., Brooks J.D.: Application of fed-batch culture to citric acid production byAspergillus niger: The effects of dilution rate and dissolved oxygen tension.Biotechnol. Bioeng. 32, 220–226 (1988).

    Article  CAS  PubMed  Google Scholar 

  • Dawson M.W., Maddox I.S., Brooks J.B.: Evidence for nitrogen catabolite repression during citric acid production byAspergillus niger under phosphate-limited growth conditions.Biotechnol. Bioeng. 33, 1500–1504 (1989).

    Article  CAS  PubMed  Google Scholar 

  • Eikmeier H., Rehm H.J.: Semicontinuous and continuous production of citric acid with immobilized cells ofAspergillus niger.Z. Naturforsch. 42C, 408–413 (1987).

    Google Scholar 

  • El-Sayed A.H.M.M., Rehm H.J.: Semicontinuous penicillin production by twoPenicillium chrysogenum strains immobilized in calcium alginate beads.Appl. Microbiol. Biotechnol. 26, 211–214 (1987).

    Article  CAS  Google Scholar 

  • Georgieva M., Aleksieva K., Ganchev I.: Biosynthesis of citric acid by a strain ofAspergillus niger IM-13 grown on starch hydrolysate.Biotechnol. Biotech. 2, 23–26 (1988).

    Google Scholar 

  • Hossain M., Brooks J.D., Maddox I.S.: Production of citric acid from whey permeate by fermentation usingAspergillus niger.N.Z.J. Dairy Sci. Technol. 18, 161–168 (1983).

    CAS  Google Scholar 

  • Jefferson W.E. Jr.: Steroids and other factors influencing the accumulation of asperenone and fermentation acids byAspergillus niger in replacement cultures.Biochemistry 6, 3484–3488 (1967).

    Article  PubMed  CAS  Google Scholar 

  • Kirimura K., Yaguchi T., Usami S.: Intraspecific protoplast fusion of citric acid producing strains ofAspergillus niger.J. Ferment. Technol. 64, 473–479 (1986).

    Article  Google Scholar 

  • Kirimura K., Lee S.P., Nakajima I., Kawabe S., Usami S.: Improvement in citric acid production by haploidization ofAspergillus niger diploid strains.J. Ferment. Technol. 66, 375–382 (1988a).

    Article  CAS  Google Scholar 

  • Kirimura K., Nakajima I., Lee S.P., Kawabe S., Usami S.: Citric acid production by the diploid strains ofAspergillus niger obtained by protoplast fusion.Appl. Microbiol. Biotechnol. 27, 504–506 (1988b).

    CAS  Google Scholar 

  • Kubicek C.P., Röhr M.: Influence of manganese on enzyme synthesis and citric acid accumulation byAspergillus niger.Eur. J. Appl. Microbiol. Biotechnol. 4, 167–173 (1977).

    CAS  Google Scholar 

  • Kubicek C.P., Röhr M.: The citric acid fermentation.CRC Crit. Rev. Biotechnol. 3, 331–373 (1986).

    Article  CAS  Google Scholar 

  • Kubicek-Pranz E.M., Mozelt M., Röhr M., Kubicek C.P.: Changes in the concentration of fructose-2,6-bisphosphate inAspergillus niger during stimulation of acidogenesis by elevated sucrose concentration.Biochim. Biophys. Acta 1033, 250–255 (1990).

    PubMed  CAS  Google Scholar 

  • Maddox I.S., Hossain M., Brooks J.D.: The effect of methanol on citric acid production from galactose byAspergillus niger.Appl. Microbiol. Biotechnol. 23, 203–205 (1986).

    Article  CAS  Google Scholar 

  • Manonmani H.K., Sreekantiah K.R.: Studies on the conversion of cellulose hydrolysate into citric acid byAspergillus niger.Proc. Biochem. 22, 92–94 (1987).

    CAS  Google Scholar 

  • Mart’inkov’a L., Mus’ilkov’a M., Ujcov’a E., Machek F., Seichert L.: Protoplast fusion inAspergillus niger strains accumulating citric acid.Folia Microbiol. 35, 143–148 (1990).

    Article  CAS  Google Scholar 

  • Mischak H., Kubicek C.P., Röhr M.: Citrate inhibition of glucose uptake inAspergillus niger.Biotechnol. Lett. 6, 425–430 (1984).

    Article  CAS  Google Scholar 

  • Ogawa K., Tsuchimochi M., Taniguchi K., Nakatsu S.: Interspecific hybridization ofAspergillus usamii mut.shirousamii andAspergillus niger by protoplast fusion.Agric. Biol. Chem. 53, 2873–2880 (1989).

    CAS  Google Scholar 

  • Pelechov’a J., Petrov’a L., Ujcov’a E., Martinkova L.: Selection of a hyperproducing strain ofAspergillus niger for biosynthesis of citric acid on unusual carbon substrates.Folia Microbiol. 35, 138–142 (1990).

    Article  CAS  Google Scholar 

  • Reusser F.: Stability and regeneration of microbial cultures on repeated transfer.Adv. Appl. Microbiol. 5, 189–213 (1963).

    Article  PubMed  CAS  Google Scholar 

  • Röhr M., Kubicek C.P.: Regulatory aspects of citric acid fermentation byAspergillus niger.Proc. Biochem. 16, 34–38 (1981).

    Google Scholar 

  • Schoorl N.:Chem. Weekblad 26, 130 (1929); cited by C.A. Browne, F.W. Zerban (Eds):Physical and Chemical Methods of Sugar Analysis, pp. 832–833. J. Wiley, New York and Chapman & Hall, London 1955.

    CAS  Google Scholar 

  • Tsay S.S., To, K.Y.: Citric acid production using immobilized conidia ofAspergillus niger TMB 2022,Biotechnol. Bioeng. 29, 297–304 (1987).

    Article  CAS  PubMed  Google Scholar 

  • Usami S., Taketomi N.: Production of citric acid by submerged fermentation of acid-hydrolysate of sweet potato starch. (In Japanese)Kogyo Kogaku Zasshi 63, 1602 (1960).

    CAS  Google Scholar 

  • Usami S., Fukutomi N.: Citric acid production by solid fermentation method using sugar cane bagasse and concentrated liquor of pineapple waste. (In Japanese)Hakko Kogaku 55, 44–50 (1977).

    CAS  Google Scholar 

  • Xu D.B., Kubicek C.P., Röhr M.: A comparison of factors influencing citric acid production byAspergillus niger grown in submerged culture and on filter paper.Appl. Microbiol. Biotechnol. 30, 444–449 (1989a).

    CAS  Google Scholar 

  • Xu D.B., Röhr M., Kubicek C.P.:Aspergillus niger cyclic AMP levels are not influenced by manganese deficiency and do not correlate with citric acid accumulation.Appl. Microbiol. Biotechnol. 32, 124–128 (1989b).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biotechnology, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20, Prague 4

    T. K. Nguyen, L. Mart’inkov’a, L. Seichert & F. Machek

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  1. T. K. Nguyen
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  2. L. Mart’inkov’a
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  3. L. Seichert
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  4. F. Machek
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Nguyen, T.K., Mart’inkov’a, L., Seichert, L. et al. Citric acid production byAspergillus niger using media containing low concentrations of glucose or corn starch. Folia Microbiol 37, 433–441 (1992). https://doi.org/10.1007/BF02899902

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