Abstract
Immunization with various cell-wall fractions ofM. tuberculosis H37Ra, progressively depleted of lipids (cell-wall-insoluble fraction; CWIF), soluble proteins (cell-wall core; CWC), mycolic acids and arabinogalactans (cell-wall-protein-peptidoglycan complex; CW-PPC) elicited significant levels of both humoral and cell-mediated immune response. Mice immunized with these fractions, when challenged with an LD50 dose ofM. tuberculosis H37Rv, exhibited significant protection as revealed by high survival rates and decreased bacterial load in lungs, liver and spleen, as compared to nonimmunized animals.
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Anacker R.L., Barclay W.R., Brehmer W., Larson C.L., Ribi E.: Duration of immunity to tuberculosis in mice vaccinated intravenously with oil treated cell walls ofMycobacterium bovis strain BCG.J. Immunol. 98, 1265–1273 (1967).
Barclay W.R., Anacker R.L., Brehmer W., Ribi E.: Effects of oil treated mycobacterial cell walls on the organs of mice.J. Bacteriol. 94, 1736–1745 (1967).
Barnes P.F., Mehra V., Hirschfield G.R., Fong S.J., Abou Zeid C., Rook G.A.W., Hunter S.W., Brennan P.J., Modlin R.L.: Characterization of T cell antigens associated with cell wall protein peptidoglycan complex ofMycobacterium tuberculosis.J. Immunol. 143, 2656–2662 (1989).
Collins F.M., Mackaness G.B.: The relationship of delayed hypersensitivity to acquired antituberculous immunity. I. Tuberculin sensitivity and resistance to reinfection in BCG vaccinated mice.Cell Immunol. 1, 253–265 (1970).
Daniel T.M., Janicki B.W.: Mycobacterial antigens: A review of their isolation, chemistry and immunological properties.Microbiol. Rev. 42, 84–113 (1978).
Ellner J.J., Spagnuolo P.J.: Suppression of antigen and mitogen induced human T lymphocyte DNA synthesis by bacterial lipopolysaccharides: Mediation, monocyte activation and production of prostaglandins.J. Immunol. 123, 2689–2695 (1979).
Falk R.E., Zabriskie J.B.: The capillary technique for measurement of inhibition of leucocyte migration. An assessment of cell mediated immunity, pp. 301–306 in B.R. Bloom, P.R. Glade (Eds):In Vitro Methods for Cell Mediated Immunity. Academic Press, New York 1971.
Gelber R.H., Brennan P.J., Hunter S.W., Munn M.W., Monson J.M., Murray L.P., Siu P., Tsang M., Engleman E.G., Mohagheghpour N.: Effective vaccination of mice against leprosy bacilli with subunits ofMycobacterium leprae.Infect. Immun. 58, 711–718 (1990).
Hoare D.S., Work E.: The stereoisomers of α-diaminopimelic acid.Biochem. J. 65, 441–447 (1957).
Hunter S.W., McNeil M., Modlin R.L., Mehra V., Bloom B.R., Brennan P.J.: Isolation and characterization of the highly immunogenic cell wall associated protein ofMycobacterium leprae.J. Immunol. 142, 2864–2872 (1989).
Khuller G.K., Dogra A., Malik U., Asotra S.: Macromolecular biosynthesis inMycobacterium smegmatis ATCC 607 in the presence of antibodies to mannophosphoinositides.J. Gen. Microbiol. 130, 1795–1798 (1984).
Laemmli U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature (London)227, 680–685 (1970).
Mehra V., Bloom B.R., Torigian V.K., Mandich D., Riechel M., Young S.M.M., Salgame P., Convit J., Hunter S.W., McNeil M., Brennan P.J., Rea T.H., Modlin R.L.: Characterization ofMycobacterium leprae cell wall associated proteins with the use of T lymphocyte clones.J. Immunol. 142, 2873–2878 (1989).
Melancon-Kaplan J.M., Hunter S.W., McNeil M., Stewart C., Modlin R.L., Rea T.H., Convit J., Salgame P., Mehra V., Bloom B.R., Brennan P.J.: Immunological significance ofMycobacterium leprae cell wall.Proc. Nat. Acad. Sci. USA 85, 1917–1921 (1988).
Pancholi P., Vinayak V.K., Khuller G.K.: Immunogenicity of ribonucleic acid protein fraction ofMycobacterium tuberculosis encapsulated in liposomes.J. Med. Microbiol. 29, 131–138 (1989).
Ribi E., Araskoy R.L., Brehmer W., Goode G., Larson A., List R.H., Milner K.C., Wicht W.C.: Factors influencing protection against experimental tuberculosis in mice by heat-stable cell wall vaccines.J. Bacteriol. 92, 869–879 (1966).
Roondle C.J.M., Morgan W.T.J.: The determination of glucosamine and galactosamine.Biochem. J. 61, 586–589 (1955).
Sibley L.D., Hunter S.W., Brennan P.J., Krahenbuhl J.L.: Mycobacterial lipoarabinomannan inhibits gamma interferon mediated activation of macrophages.Infect. Immun. 56, 1232–1236 (1988).
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Chugh, I.B., Kansal, R., Vinayak, V.K. et al. Protective efficacy of different cell-wall fractions ofMycobacterium tuberculosis . Folia Microbiol 37, 407–412 (1992). https://doi.org/10.1007/BF02899898
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DOI: https://doi.org/10.1007/BF02899898