Skip to main content
SpringerLink
Log in

Increased UDP-glucuronyltransferase and gamma-glutamyltranspeptidase in enzyme-altered rat liver lesions produced by low doses of aflatoxin B1

  • Published:
Virchows Archiv B

Summary

Preneoplastic liver lesions were produced in female Wistar rats by low doses of aflatoxin B1 (Model 1: administration of 37.5 μg/kg 12 and 24 h after partial hepatectomy; Model 2: continuous application of 3.5 μg/kg in tap water daily for 28 days with partial hepatectomy after 14 days. The animals then received sodium phenobarbital, 0.1% in tap water, for 180 to 400 days). In both models numerous altered hepatic foci (AHF) and hyperplastic nodules (HN) were detected enzyme histochemically by their negative ATPase and positive gamma-glutamyl-transpeptidase reactions. Immunohistochemically these lesions were also UDP-glucuronyltransferase positive. Increased UDP-glucuronyltransferase adds to permanent alterations of a number of drug metabolizing enzymes observed in a variety of different tumor models. These alterations are responsible for the toxin-resistant phenotype (Faber 1984b). Increased gamma-glutamyltranspeptidase was detected both enzyme histochemically and immunohistochemically; whereas gamma-glutamyl-transpeptidase activity was present in both AHF/HN and in periportal areas by enzyme histochemistry, the immunohistochemical method selectively stained gamma-glutamyltranspeptidase in AHF and HN. Immunohistochemically detectable UDP-glucuronyltransferase and gamma-glutamyltranspeptidase are markers of putative precancerous liver lesions which may be useful in the analysis of the prestages of liver carcinogenesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Angsubhakorn S, Bhamarapravati N, Romruen K, Sahaphong S, Thamavit W, Miyamoto M (1981) Further study of a benzene hexachloride inhibition of aflatoxin B1 hepatocarcinogenesis in rats. Br J Cancer 43:881–883

    PubMed  CAS  Google Scholar 

  • Aronsen KF, Nosslin B, Pihl B (1970) The value of γ-glutamyl-transpeptidase as a screen test for liver tumour. Acta Chir Scand 136:17–22

    PubMed  CAS  Google Scholar 

  • Bannasch P (1968) The cytoplasm of hepatocytes during carcinogenesis. Recent Results in Cancer Research, vol. 19. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Bannasch P, Papenburg J, Ross W (1972) Cytomorphologische und morphometrische Studien der Hepatocarcinogenese. I. Reversible und irreversible Veränderungen am Cytoplasma der Leberparenchymzellen bei Nitrosomorpholin-vergifteten Ratten. Z Krebsforsch 77:108–133

    Article  CAS  Google Scholar 

  • Bannasch P, Benner U, Enzmann H, Hacker HJ (1985) Tigroid cell foci and neoplastic nodules in the liver of rats treated with a single dose of aflatoxin B1. Carcinogenesis 6:1641–1648

    Article  PubMed  CAS  Google Scholar 

  • Berenblum I, Shubik P (1947) The role of croton oil application, associated with a single painting of a carcinogen, in tumor induction of the mouse’s skin. Br J Cancer 1:379–382

    PubMed  CAS  Google Scholar 

  • Bergmann FH, Levine L, Spiro RG (1962) Fetuin: immunochemistry and quantitative estimation in serum. Biochim Biophys Acta 58:41–52

    Article  PubMed  CAS  Google Scholar 

  • Bock KW, Josting D, Lilienblum W, Pfeil H (1979) Purification of rat-liver microsomal UDP-glucuronyltransferase. Eur J Biochem 98:19–26

    Article  PubMed  CAS  Google Scholar 

  • Bock KW, Lilienblum W, Pfeil H, Eriksson LC (1982) Increased uridine diphosphate glucuronyltransferase activity in preneoplastic liver nodules and morris hepatomas. Cancer Res 42:3747–3752

    PubMed  CAS  Google Scholar 

  • Butler WH (1969) Aflatoxicosis in laboratory animals. In: Goldblatt LA (ed) Aflatoxin. Academic Press, New York, pp 223–236

    Google Scholar 

  • Butler WH, Barnes JM (1963) Toxic effects of groundnut meal containing aflatoxin to rats and guinea-pigs. Br J Cancer 17:699

    PubMed  CAS  Google Scholar 

  • Butler WH, Hempsall V, Stewart MG (1981) Histochemical studies on the early proliferative lesion induced in the rat liver by aflatoxin. J Pathol 133:325–340

    Article  PubMed  CAS  Google Scholar 

  • Cameron R, Kellen J, Kolin A, Malkin A, Farber E (1978) γ-glutamyltransferase in putative premalignant liver cell populations during hepatocarcinogenesis. Cancer Res 38:823–829

    PubMed  CAS  Google Scholar 

  • Cameron R, Sweeney GD, Jones K, Lee G, Farber E (1976) A relative deficiency of cytochrome P-450 and aryl hydrocarbon (benzo(a)pyrene)hydroxylase in hyperplastic nodules induced by 2-acet-aminofluorene in rat liver. Cancer Res 36:3888–3893

    PubMed  CAS  Google Scholar 

  • Campbell HA, Pitot HC, Potter R, Laishes BA (1982) Application of quantitative stereology to the evaluation of enzyme-altered foci in rat liver. Cancer Res 42:465–472

    PubMed  CAS  Google Scholar 

  • Capouya ER, Lindahl R (1983) Changes in γ-glutamyltransferase activity during N-2-fluorenyl-acetamide-induced rat hepatocarcinogenesis. JNCI 70:359–365

    PubMed  CAS  Google Scholar 

  • Cheng S, Nassar K, Levy D (1978) γ-Glutamyltranspeptidase activity in normal regenerating and malignant hepatocytes. FEBS Lett 85:310–312

    Article  PubMed  CAS  Google Scholar 

  • Cohen GM, Gibby EM, Mehta R (1981) Route of conjugation in normal and cancerous tissue from human lung. Nature 291:662–664

    Article  PubMed  CAS  Google Scholar 

  • Deml E, Oesterle D (1980) Histochemical demonstration of enhanced glutathione content in enzyme-altered islands induced by carcinogenesis in rat liver. Cancer Res 40:490–491

    PubMed  CAS  Google Scholar 

  • Emmelot P, Scherer E (1980) The first relevant cell stage in rat liver carcinogenesis. Biochim Biophys Acta 605:247–304

    PubMed  CAS  Google Scholar 

  • Enomoto K, Ying TS, Griffin MJ, Farber E (1981) Immunohistochemical study of epoxide hydrolase during experimental liver carcinogenesis. Cancer Res 41. 3281–3287

    PubMed  CAS  Google Scholar 

  • Eriksson LC, Astrom A, De Pierre JW, Bock KW (1981) Induction of drug metabolizing enzymes in preneoplastic liver nodules. Biochem Soc Trans 9:271

    Google Scholar 

  • Farber E (1956) imilarities in the sequence of early histological changes induced in the liver of the rat by ethionine, 2-acetylamino-fluorene, and 3′-methyl-4-dimethylaminoazobenzene. Cancer Res 16:142–149

    PubMed  CAS  Google Scholar 

  • Farber E (1977) Experimental liver carcinogenesis: A perspective. Remmer H, Bannasch P, Bolt HM, Popper H (eds) Primary liver tumors. Falk Symposion 25. MTP Press Ltd., Lancaster, pp 357–375

    Google Scholar 

  • Farber E (1982) The sequential analysis of cancer development in liver. 13th International Cancer Congress. Proceedings: 81

  • Farber E (1984a) Chemical carcinogenesis: a current biological perspective. Carcinogenesis 5:1–5

    Article  PubMed  CAS  Google Scholar 

  • Farber E (1984b) The multistep nature of cancer development. Cancer Res 44:4217–4223

    PubMed  CAS  Google Scholar 

  • Farber E (1984c) Cellular biochemistry of the stepwise development of cancer with chemicals. Cancer Res 44:5463–5474

    PubMed  CAS  Google Scholar 

  • Farber E, Cameron RG (1980) The sequential analysis of cancer development. Adv Cancer Res 31:125–226

    PubMed  CAS  Google Scholar 

  • Fiala S, Fiala AE, Dixon B (1972) γ-glutamyltranspeptidase in transplantable, chemically induced rat hepatomas and “spontaneous” mouse hepatomas. J Natl Cancer Inst 48:1393–1401

    PubMed  CAS  Google Scholar 

  • Fiala S, Mohindru A, Kettering WG, Fiala AE, Morris HP (1976) Glutathione and gamma glutamyl transpeptidase in rat liver during chemical carcinogenesis. J Natl Cancer Inst 57:591–598

    PubMed  CAS  Google Scholar 

  • Fischer G, Altmannsberger M, Katz N, Schauer A (1983a) Early stages of chemically-induced liver carcinogenesis by oral administration of the antihistaminic methapyrilene hydrochloride. J Cancer Res Clin Oncol 106:53–57

    Article  PubMed  CAS  Google Scholar 

  • Fischer G, Schauer A, Bock KW, Ullrich D, Katz N (1983b) Immunohistochemical demonstration of increased UDP-glucuronyltransferase in putative preneoplastic liver foci. Naturwissenschaften 70:153

    Article  PubMed  CAS  Google Scholar 

  • Fischer G, Ullrich D, Katz N, Bock KW, Schauer A (1983c) Immunohistochemical and biochemical detection of uridine-diphosphate-glucuronyltransferase (UDP-GT) activity in putative preneoplastic liver foci. Virchows Arch [Cell Pathol] 42:193–200

    CAS  Google Scholar 

  • Fischer G, Ullrich D, Lilienblum W, Bock KW, Schauer A, Katz N (1983d) Quantitativ-histochemische und biochemische Untersuchungen zur methapyrileninduzierten Lebercarcinogenese der Ratte. Verh Dtsch Ges Pathol 67:637

    Google Scholar 

  • Fischer G, Schauer A, Hartmann H, Bock KW (1985a) Increased UDP-glucuronyltransferase in putative preneoplastic foci of human liver after long-term administration of oral contraceptives. Naturwissenschaften 72:277–278

    Article  PubMed  CAS  Google Scholar 

  • Fischer G, Ullrich D, Bock KW (1985b) Effects of N-nitrosomorpholine and phenobarbital on UDP-glucuronyltransferase in putative preneoplastic foci of rat liver. Carcinogenesis 6:605–609

    Article  PubMed  CAS  Google Scholar 

  • Fischer G, Lilienblum W, Ullrich D, Bock KW (1986a) Immunohistochemical differentiation of γ-glutamyltranspeptidase in focal lesions and in zone I of rat liver after initiation by chemical carcinogens. Carcinogenesis (in press)

  • Fischer G, Hartmann H, Droese M, Schauer A, Bock KW (1986b) Histochemical and immuno-histochemical detection of putative preneoplastic liver foci in women after long-term use of oral contraceptives. Virchow’s Arch [Cell Pathol] 50:321–337

    CAS  Google Scholar 

  • Gerber MA, Thung SN (1980) Enzyme patterns in human hepatocellular carcinoma. Am J Pathol 98:395–400

    PubMed  CAS  Google Scholar 

  • Goldfarb SA (1973) Morphological and histochemical study of carcinogenesis of the liver in rats fed 3′-methyl-4-dimethylaminoazobenzene. Cancer Res 33:1119–1128

    PubMed  CAS  Google Scholar 

  • Goldfarb S, Pugh TD (1981) Enzyme histochemical phenotypes in primary hepatocellular carcinomas. Cancer Res 41:2092–2095

    PubMed  CAS  Google Scholar 

  • Goldsworthy TL, Pitot HC (1985) The quantitative analysis and stability of histochemical markers of altered hepatic foci in rat liver following initiation by diethylnitrosamine administration and promotion with phenobarbital. Carcinogenesis 6:1261–1269

    Article  PubMed  CAS  Google Scholar 

  • Hanigan MH, Pitot HC (1985) Gamma-glutamyl transpeptidase —its role in hepatocarcinogenesis. Carcinogenesis 6:165–172

    Article  PubMed  CAS  Google Scholar 

  • Hattori N, Sawabu N, Nakagen M, Ichii M (1978) Novel gammaglutamyl transpeptidase isoenzyme occurring in sera of patients with hepatoma. Scand J Immunol 8 [Suppl 8]: 507–510

    Google Scholar 

  • Higgins GM, Anderson RM (1931) Experimental pathology of the liver. 1. Restoration of the liver of the white rat following partial surgical removal. Arch Pathol 12:186–202

    Google Scholar 

  • Hixson DC, Allison JP (1985) Monoclonal antibodies recognizing oval cells induced in the liver of rats by N-2-fluorenylacetamide or ethionine in a cholin-deficient diet. Cancer Res 45:3750–3760

    PubMed  CAS  Google Scholar 

  • Igarashi T, Satoh T, Ueno K, Kitagawa H (1981) Changes of γ-glutamyltranspeptidase activity in the rat during development and comparison of the fetal liver, placental and adult liver enzymes. Life Sci 29:483–491

    Article  PubMed  CAS  Google Scholar 

  • Iype TP, Tomaszewski JE, Dipple A (1979) Biochemical basis for cytotoxicity of 7,12-dimethylbenz(a)anthracene in rat liver epithelial cells. Cancer Res 39:4925–4929

    PubMed  CAS  Google Scholar 

  • Jalonka H, Ruoslahti E (1979) Differential expression of alpha-fetoprotein and gamma-gluta-myltranspeptidase in chemical and spontaneous hepatocarcinogenesis. Cancer Res 39:3495–3501

    Google Scholar 

  • Kalengayi MMR, Desmet VJ (1975a) Sequential histological and histochemical study of the rat liver after single-dose aflatoxin B1 intoxication. Cancer Res 35:2836–2844

    PubMed  CAS  Google Scholar 

  • Kalengayi MMR, Desmet VJ (1975b) Sequential histological and histochemical study of the rat liver during aflatoxin B1-induced carcinogenesis. Cancer Res 35:2845–2852

    PubMed  CAS  Google Scholar 

  • Kalengayi MMR, Desmet VJ (1978) Gamma-glutamyl transferase as oncofetal marker of experimental hepatocarcinogenesis in the rat. Scand J Immunol 8 [Suppl 8]: 547–556

    CAS  Google Scholar 

  • Kalengayi MM, Ronchi G, Desmet VJ (1975) Histochemistry of gamma-glutamyl transpeptidase in rat liver during aflatoxin B1-induced carcinogenesis. J Natl Cancer Inst 55:579–588

    PubMed  CAS  Google Scholar 

  • Kitagawa T, Watanabe R, Sugano H (1980) Induction of γ-glutamyltranspeptidase activity by dietary phenobarbital in “spontaneous” hepatic tumors of C3H mice. Gann 71:536–542

    PubMed  CAS  Google Scholar 

  • Kuhlmann WD, Krischan R, Kunz W, Guenthner TM, Oesch F (1981) Focal elevation of liver microsomal epoxide hydrolase in early preneoplastic stages and its behaviour in the further course of heptocarcinogenesis. Biochem Biophys Res Commun98:2:417–423

    Article  PubMed  CAS  Google Scholar 

  • Köttgen E, Reutter W, Gerok W (1976) Two different gamma-glutamyltransferases during development of liver and small intestine: A fetal (sialo-) and an adult (asialo-) glycoprotein. Biochem Biophys Res Commun 72:61–66

    Article  PubMed  Google Scholar 

  • Levin K, Lu AYH, Thomas PE, Ryan D, Kizer DE, Griffin MJ (1978) Identification of epoxide hydrase as the preneoplastic antigen in rat liver hyperplastic nodules. Proc Natl Acad Sci (USA) 75:3240–3243

    Article  CAS  Google Scholar 

  • Lipsky M, Hinton D, Klaunig J, Goldblatt P, Trump B (1980) Gamma glutamyltranspeptidase in safrole-induced presumptive premalignant mouse hepatocytes. Carcinogenesis 1:151–156

    Article  CAS  Google Scholar 

  • Lueders KK, Dyer HM, Thompson EB, Kuff EL (1970) Glucuronyltransferase activity in transplantable rat hepatomas. Cancer Res 30:274–279

    PubMed  CAS  Google Scholar 

  • Mahieu PM, Lambert PH, Maghuin-Rogister GR (1973) Primary structure of a small glycopeptide isolated from human glomerular basement membrane and carrying a major antigenic site. Eur J Biochem 40:599–606

    Article  PubMed  CAS  Google Scholar 

  • Miller EC (1978) Some current perspectives on chemical carcinogenesis in humans and experimental animals. Cancer Res 38:1479–1496

    PubMed  CAS  Google Scholar 

  • Miller F (1971) Glycopeptides of human immunoglobulins-II. Contribution to the antigenicity of the heavy chain. Immunochemistry 8:99–111

    Article  PubMed  CAS  Google Scholar 

  • Newberne PM, Butler WH (1969) Acute and chronic effects of aflatoxin on the liver of domestic and laboratory animals. A review. Cancer Res 29:236–250

    CAS  Google Scholar 

  • Nixon JE, Hendricks JD, Pawlowski NE, Loveland PM, Sinnhuber RO (1981) Carcinogenicity of aflatoxicol in Fischer 344 rats. JNCI 66:1159–1163

    PubMed  CAS  Google Scholar 

  • Novikoff AB, Novikoff PM, Stockert RJ, Becker FF, Yam A, Poruchynsky MS, Levin W, Thomas PE (1979) Immunocytochemical localization of epoxide hydrase in hyperplastic nodules induced in rat liver by 2-acetylaminofluorene. Proc Natl Acad Sci (USA) 76:5207–5211

    Article  CAS  Google Scholar 

  • Pereira MA, Herren-Freund SL, Britt AL, Khoury MM (1984) Effect of coadministration of phénobarbital sodium on N-nitrosodiethylamine-induced γ-glytamyltransferase-positive foci and hepatocellular carcinoma in rats. JNCI 72:741–744

    PubMed  CAS  Google Scholar 

  • Pfeil H, Bock KW (1983) Electroimmunochemical quantification of UDP-glucuronyltransferase in rat liver microsomes. Eur J Biochem 131:619–623

    Article  PubMed  CAS  Google Scholar 

  • Pitot HC (1980) Characteristics of stages of hepatocarcinogenesis. In: B Pullman, POP Ts’o, Gelboin H (eds) Carcinogenesis. Fundamental mechanisms and environmental effects. D Reidel Publishing Co Inc, Hingham Mass, pp 219–233

    Google Scholar 

  • Pitot HC, Sirica AE (1980) The stages of initiation and promotion in hepatocarcinogenesis. Biochim Biophys Acta 605:191–215

    PubMed  CAS  Google Scholar 

  • Pugh TD, Goldfarb S (1978) Quantitative histochemical and autoradiographic studies of hepatocarcinogenesis in rats fed 2-acetyl-aminofluorene followed by phenobarbital. Cancer Res 38:4450–4457

    PubMed  CAS  Google Scholar 

  • Rao MS, Lalwani ND, Scarpelli DG, Reddy JK (1982) The absence of γ-glutamyl transpeptidase activity in putative preneoplastic lesions and in hepatocellular carcinomas induced in rats by the hypolipidemic peroxisome proliferator Wy-14,643. Carcinogenesis 3:1231–1233

    Article  PubMed  CAS  Google Scholar 

  • Ratanasavanh D, Tazi A, Galteau MM, Siest G (1979) Localization of gamma-glutamyltransferase in subcellular fractions of rat and rabbit liver: effect of phenobarbital. Biochem Pharmacol 28:1363–1365

    Article  PubMed  CAS  Google Scholar 

  • Roomi MW, Goldberg DM (1981) Comparison of gamma-glutamyltransferase induction by phenobarbital in the rat, guinea pig and rabbit. Biochem Pharmacol 30:1563–1571

    Article  PubMed  CAS  Google Scholar 

  • Rutenburg AM, Kim H, Fischbein JW, Hanker JS, Wasserkrug HC, Seligman AM (1969) Histochemical and ultrastructural demonstration of gamma-glutamyl transpeptidase activity. J Histochem Cytochem 17:517–526

    PubMed  CAS  Google Scholar 

  • Schauer A, Kunze E (1968) Enzymhistochemische und autoradiographische Untersuchungen während der Cancerisierung der Rattenleber mit Diäthylnitrosamin. Z Krebsforsch 70:252–266

    Article  PubMed  CAS  Google Scholar 

  • Scherer E (1984) Neoplastic progression in experimental hepatocarcinogenesis. Biochim Biophys Acta 738:219–236

    PubMed  CAS  Google Scholar 

  • Schulte-Hermann R, Ohde G, Schuppler J, Timmermann-Trosiener I (1981) Enhanced proliferation of putative preneoplastic cells in rat liver following treatment with the tumor promoters phenobarbital, hexachlorocyclohexane, steroid compounds, and nafenopin. Cancer Res 41:2556–2562

    PubMed  CAS  Google Scholar 

  • Sell S, Leffert HL (1982) An evaluation of cellular lineages in the pathogenesis of experimental hepatocellular carcinoma. Hepatology 2:77–86

    Article  PubMed  CAS  Google Scholar 

  • Silbernagl S, Pfaller W, Heinle H, Wendel A (1978) Topology and function of renal γ-glutamyl transpeptidase. In: Sies H, Wendel A (eds). Function of glutathione in liver and kidney. Springer, Berlin Heidelberg New York, pp 60–69

    Google Scholar 

  • Spater HW, Poruchynsky MS, Quintana N, Inoue M, Novikoff AB (1982) Immunocytochemical localization of γ-glutamyltransferase in rat kidney with protein A-horseradish peroxidase. Proc Natl Acad Sci (USA) 79:3547–3550

    Article  CAS  Google Scholar 

  • Spater HW, Quintana N, Becker FF, Novikoff AB (1983) Immunocytochemical localization of γ-glutamyltransferase in induced hyperplastic nodules of rat liver. Proc Natl Acad Sci (USA) 80:4742–4746

    Article  CAS  Google Scholar 

  • Szewczuk A, Connell GE (1964) The effect of neuraminidase on the properties of gamma-glutamyl transpeptidase. Biochim Biophys Acta 83:218–223

    PubMed  CAS  Google Scholar 

  • Taniguchi N, Saito K, Takakuwa E (1975) γ-glutamyltransferase from azo dye induced hepatoma and fetal rat liver. Similarities in their kinetic and immunological properties. Biochim Biophys Acta 391:265–271

    PubMed  CAS  Google Scholar 

  • Taniguchi N, Tsukada Y, Mukuo K, Hirai H (1974) Effects of hepatocarcinogenic azo dye on glutathione and related enzymes in rat liver. Gann 65:381–387

    PubMed  CAS  Google Scholar 

  • Tatematsu M, Kaku T, Medline A, Eriksson L, Roomi W, Sharma RN, Murray RK, Farber E (1983a) Markers of liver neoplasia —real of fictional? In: Wilson CM (ed) Application of biological markers to carcinogen testing. Plenum Press, New York

    Google Scholar 

  • Tatematsu M, Nagamine Y, Farber E (1983b) Redifferentiation as a basis for remodeling of carcinogen-induced hepatocyte nodules to normalappearing liver. Cancer Res 43:5049–5058

    PubMed  CAS  Google Scholar 

  • Tatematsu M, Kaku T, Farber E (1984) Studies on the proliferation and fate of oval cells in the liver of rats treated with 2-acetylaminofluorene and partial hepatectomy. Am J Pathol 114:418–430

    PubMed  CAS  Google Scholar 

  • Taylor CR, Burns J (1974) The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxydase-labeled antibody. J Clin Pathol 27:14–20

    Article  PubMed  CAS  Google Scholar 

  • Tazi A, Galteau M, Siest G (1980) Gamma-glutamyltransferase of rabbit liver: kinetic study of phenobarbital induction and in vitro solubilization by bile salts. Toxicol Appl Pharmacol 55:1–7

    Article  PubMed  CAS  Google Scholar 

  • Tsao M-S, Smith JD, Nelson KG, Grisham JW (1984) A diploid epithelial cell line from normal adult rat liver with phenotypic properties of oval cells. Exp Cell Res 154:38–52

    Article  PubMed  CAS  Google Scholar 

  • Tsuchida S, Hoshino K, Sato T, Ito N, Sato K (1979) Purification of γ-glutamyltransferases from rat hepatomas and hyperplastic hepatic nodules, and comparison with the enzyme from rat kidney. Cancer Res 39:4200–4205

    PubMed  CAS  Google Scholar 

  • Tsuda H, Lee G, Farber E (1980) Induction of resistant hepatocytes as a new principle for a possible short-term in vivo test for carcinogens. Cancer Res 40:1157–1164

    PubMed  CAS  Google Scholar 

  • Uchida E, Hirono I (1981) Effect of phenobarbital on the development of neoplastic lesions in the liver of cycasin-treated rats. J Cancer Res Clin Oncol 100:231–238

    Article  PubMed  CAS  Google Scholar 

  • Ullrich D, Fischer G, Katz N, Bock KW (1984) Intralobular distribution of UDP-glucuronyl-transferase in liver from untreated, 3-methylcholanthrene-and phenobarbital-treated rats. Chem Biol Interact 48:181–190

    Article  PubMed  CAS  Google Scholar 

  • Wachstein M, Meisel E (1957) Histochemistry of hepatic phosphatases at a physiologic pH. Am J Clin Pathol 27:13–23

    PubMed  CAS  Google Scholar 

  • Wapnir RA, Mancusi VJ, Goldstein LA (1982) Comparative ontogenesis of gamma-glutamyl transpeptidase in rat tissues. Experientia 38:647–648

    Article  PubMed  CAS  Google Scholar 

  • Watkins WM (1976) Blood group substances. Science 152:172–181

    Article  Google Scholar 

  • Yamashita K, Hitoi A, Matsuda Y, Tsuji A, Katunuma N, Kobata A (1983a) Structural studies of the carbohydrate moieties of rat kidney γ-glutamyl transpeptidase. An extremely heterogeneous pattern enriched with nonreducing terminal N-acetylglucosamine residues. J Biol Chem 258:1098–1107

    PubMed  CAS  Google Scholar 

  • Yamashita K, Hitoi A, Taniguchi N, Yokosawa N, Tsukada Y, Kobata A (1983b) Comparative study of the sugar chains of γ-glutamyltranspeptidases purified from rat liver and rat AH-66 hepatoma cells. Cancer Res 43:5059–5063

    PubMed  CAS  Google Scholar 

  • Yin Z, Sato K, Tsuda H, Ito N (1982) Changes in activities of uridine diphosphate-glucuronyl-transferase during chemical hepatocarcinogenesis. Gann 73:239–248

    PubMed  CAS  Google Scholar 

  • Ying TS, Sarma DSR, Farber E (1981) Role of acute hepatic necrosis in the induction of early steps in liver carcinogenesis by diethylnitrosamine. Cancer Res 41:2096–2102

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Pathology, University of Göttingen, Robert-Koch-Straße 40, D-3400, Göttingen, Federal Republic of Germany

    Gösta Fischer

Authors
  1. Gösta Fischer
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Dedicated to Prof. Dr. med. Steffen Berg on the occasion of his 65th birthday

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fischer, G. Increased UDP-glucuronyltransferase and gamma-glutamyltranspeptidase in enzyme-altered rat liver lesions produced by low doses of aflatoxin B1 . Virchows Archiv B Cell Pathol 51, 443–460 (1986). https://doi.org/10.1007/BF02899051

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02899051

Key words

Search

Navigation