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Effets de l’Oxygenation du Milieu d’Incubation des Cotyledons de Ricin sur les Flux de Protons et sur l’Absorption du Saccharose

Effects of oxygenation of castor bean cotyledons on proton and sucrose influx

  • Original Papers
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Biologia Plantarum

Abstract

Some of the effects of oxygen concentration in the external medium bathing castor bean cotyledons were investigated.

At 700 μM O2, the sucrose-dependent proton influx was dependent on external pH while sucrose influx was independent of pH between 4,5 and 6; proton influx and sucrose influx were both dependent on external sucrose concentration; both were saturated above 50 mM sucrose. The stoichiometry H+/S decreased with increasing pH and sucrose concentration. These data were consistent with the results obtained in stirred or aerated external medium.

Oxygen consumption, acidification of the medium, sucrose-dependent proton influx and sucrose uptake increased with increasing oxygen concentration up to 700 μM where saturation was reached. Bubbling with air (O2, 160 μM) resulted in the same increase despite lower oxygen concentration. It is suggested that not only O2 concentration but also CO2 concentration act on O2 consumption, and proton and sucrose fluxes.

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Abbreviations

FFCP:

carbonyl cyanure p-(trifluoromethoxy)-phenylhydrazone

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Authors and Affiliations

  1. Laboratoire de Botanique et Biologie végétale, Université Scientifique et Médicale de Grenoble, BP 68, 38402, Saint-Martin D’Hères Cédex, France

    Françoise de Bock

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  1. Françoise de Bock
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de Bock, F. Effets de l’Oxygenation du Milieu d’Incubation des Cotyledons de Ricin sur les Flux de Protons et sur l’Absorption du Saccharose. Biol Plant 26, 327–336 (1984). https://doi.org/10.1007/BF02898567

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  • DOI: https://doi.org/10.1007/BF02898567

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