Abstracts
Ricinus cotyledons were used to investigate whether sucrose uptake by the phloem could be due to H+-sucrose cotransport. The addition of sucrose to the medium bathing Ricinus cotyledons created a pH shift to more alkaline values. The effect was not observed on the addition of glucose or fructose. Net H+ influx and sucrose influx were both shown to be temperature sensitive, dependent on pH and external sucrose concentration. However, net H+ influx was far more sensitive to pH than sucrose influx and was abolished at high pH; it was also observed for a shorter time period than sucrose uptake. The stoichiometry of H+: sucrose was less than one and declined with increasing sucrose concentration and increasing pH. Two possible models are proposed to account for this low and changing stoichiometry. Given and H+ pump, recirculation would seem an inevitable consequence of sucrose and proton cotransport.
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References
Anderson, W.P.: Electrogenic pump current in higher plant cells. In: Transmembrane ionic exchanges in plants, Colloques int. Cent. natn. Rech. Scient., Paris. In press (1976)
Boos, W.: Bacterial transport. Ann. Rev. Biochem. 43, 123–146 (1974)
Eddy, A.A., Nowacki, J.A.: Stoichiometric proton and potassium movements accompanying the absorption of amino acids in the yeast Saccharomyces carlsbergensis. Biochem. J. 122, 701–711 (1971)
Etherton, B., Nuovo, C.: Rapid changes in membrane potentials of oat coleoptile cells induced by amino acids and carbohydrates. Plant Physiol. 54S, 49 (1974)
Geiger, D.R.: Phloem loading. In: Transport in plants. I, pp. 395–431. Zimmermann, M.H., Milburn, J.A., eds., Berlin-Heidelberg-New York: Springer 1975
Giaquinta, R.T.: Possible role of pH gradient and membrane ATPase in the loading of sucrose into the sieve tubes. Nature 267, 369–370 (1977)
Hamilton, W.A.: Energy coupling in microbial transport. Adv. Microbiol. Physiol. 12, 1–53 (1975)
Higinbotham, N., Anderson, W.P.: Electrogenic pumps in higher plant cells. Can. J. Bot. 52, 1011–1021 (1974)
Hodges, T.K.: ATPases associated with membranes of plant cells. In: Transport in Plants II, Part A, Cells, pp. 260–283. Lüttge, U., Pitman, M.G., eds., Berlin-Heidelberg-New York: Springer 1976
Jones, M.G.K., Novacky, A., Dropkin, V.H.: Transmembrane potentials of parenchyma cells and nematode-induced transfer cells. Protoplasma 85, 15–37 (1975)
Komor, E.: Sucrose uptake by cotyledons of Ricinus communis L.: Characteristics, mechanism, and regulation. Planta 137, 119–131 (1977)
Komor, E., Tanner, W.: Proton movement associated with hexose transport in Chlorella vulgaris. In: Membrane Transport in Plants, pp. 209–215. Zimmermann, U., Dainty, J., eds., Berlin-Heidelberg-New York: Springer (1974a)
Komor, E., Tanner, W.: The hexose-proton symport system of Chlorella vulgaris. Eur. J. Biochem. 44, 219–223 (1974b)
Komor, E., Tanner, W.: The hexose-proton cotransport system of Chlorella: pH dependent change in K m values and translocation constants of the uptake system. J. Gen. Physiol. 64, 568–581 (1974c)
Komor, E., Tanner, W.: Simulation of a high- and low-affinity sugar-uptake system in Chlorella by a pH-dependent change in the K m of the uptake system. Planta 123, 195–198 (1975)
Komor, E., Rotter, M., Tanner, W.: A proton-cotransport system in a higher plant: sucrose transport in Ricinus communis. Plant Sci. Lett. 9, 153–162 (1977)
Kriedemann, P.: Sugar uptake and translocation in the castor bean seedling. III. An autoradiographic study of the absorption pathway. Planta 73, 175–180 (1967)
Kriedemann, P., Beevers, H.: Sugar uptake and translocation in the castor bean seedling. I. Characteristics of transfer in intact and excised seedlings. Plant Physiol. 42, 161–173 (1967a)
Kriedemann, P., Beevers, H.: Sugar uptake and translocation in the castor bean seedling. II. Sugar transformations during uptake. Plant Physiol. 42, 174–180 (1967b)
MacRobbie, E.A.C.: Ion transport in plant cells. Current Topics in Membranes and Transport 7, 1–48 (1975)
MacRobbie, E.A.C.: The functions of ion transport in plant cells and tissues. MTP Int. Rev. Sci. Biochem. 13, 211–247 (1976)
Mitchell, P.: Vectorial chemistry and molecular mechanics of chemi-osmotic coupling: Power transmission by proticity. Biochem. Soc. Transact. 4, 399–430 (1976)
Patterson, M.S., Greene, R.C.: Measurement of low energy betaemitters in aqueous solution by liquid scintillation counting of emulsions. Anal. Chem. 37, 854–857 (1965)
Racusen, R.H., Galston, A.W.: Electrical evidence for rhythmic changes in the cotransport of sucrose and hydrogen ions in Samanea pulvini. Planta 135, 57–62 (1977)
Raven, J.A., Smith, F.A.: Cytoplasmic pH regulation and electrogenic H+ extrusion. Curr. Adv. Plant Sci. 8, 649–660 (1976a)
Raven, J.A., Smith, F.A.: Characteristics, functions and regulation of active proton extrusion. In: Regulation of Cell Membrane Activities in Plants, pp. 25–40. Marrè, E., Ciferri, O., eds., Amsterdam: North Holland 1976b
Seaston, A., Inkson, C., Eddy, A.A.: The absorption of protons with specific amino acids and carbohydrates by yeast. Biochem. J. 134, 1031–1043 (1973)
Seaston, A., Carr, G., Eddy, A.A.: The concentration of glycine by preparations of the yeast Saccharomyces carlsbergensis depleted of adenosine triphosphate. Biochem. J. 154, 669–676 (1976)
Simoni, R.D., Postma, P.W.: The energetics of bacterial active transport. Ann. Rev. Biochem. 44, 523–554 (1975)
Slayman, C.L.: Proton pumping and generalized energetics of transport: a review. In: Membrane Transport in Plants, pp. 107–119. Zimmermann, U., Dainty, J., eds., Berlin-Heidelberg-New York: Springer 1974
Slayman, C.L., Slayman, C.W.: Depolarization of the plasma membrane of Neurospora during active transport of glucose: Evidence for a proton-dependent cotransport system. Proc. Natl. Acad. Sci. USA 71, 1935–1939 (1974)
Smith, F.A., Raven, J.A.: H+ transport and regulation of cell pH. In: Transport in Plant Cells II, Part A, Cells, pp. 317–346. Lüttge, U., Pitman, M.G., eds., Berlin-Heidelberg-New York: Springer 1976
Spanswick, R.M.: Evidence for an electrogenic ion pump in Nitella translucens. I. The effects of pH, K+, Na+, light and temperature on the membrane potential and resistance. Biochim. Biophys. Acta. 288, 73–89 (1972)
Spanswick, R.M.: Electrogenesis in photosynthetic tissue. In: Ion Transport in Plants, pp. 113–128. Anderson, W.P., ed. New York: Academic Press 1973
Spanswick, R.M.: Evidence for an electrogenic pump in Nitella translucens. II. The control of the light stimulated component of the membrane potential. Biochim. Biophys. Acta 332, 387–398 (1974)
Ziegler, H.: Nature of transported substances. In: Transport in Plant Cells I, pp. 59–100. Zimmermann. M.H., Milburn, J.A., eds., Berlin-Heidelberg-New York: Springer 1975
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Hutchings, V.M. Sucrose and proton cotransport in Ricinus cotyledons. Planta 138, 229–235 (1978). https://doi.org/10.1007/BF00386816
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DOI: https://doi.org/10.1007/BF00386816