Skip to main content
SpringerLink
Log in

Regeneration of plants from leaf mesophyll protoplasts of the tetraploid potato cultivars Xenia and Bintje

  • Published:
Biologia Plantarum

Abstract

An improved method for the isolation of leaf mesophyll protoplasts ofSolanum tuberosum L. cvs. Xenia and Bintje, their culture and regeneration to plants is described. The improvements involve the culture of nodal stem explants on Schenk and Hildebrandt (SH) medium supplemented with AgNO3 (7.5 mg 11) and Alar 85 (5 mg 1−1), and the pre-treatment of donor plants for 2–3 weeks at a short photoperiod (6 h), a low irradiance (17 (μmol m−2s−1) and a low temperature (20 °C). Regeneration of cell wals was observed within 2–3 d and cell divisions within 7–12 d after resuspending freshly isolated protoplasts in Sidorov et al. (SW) medium. The resulting microcalli were cultured on solid media containing zeatin (1–2,5 mg 1−1) and/or NAA (0.1 and/or 0.01 mg 1−1) and subsequently on medium with BAR (0.25 mg1−1) and GA−3 (0.1 mg 11) for shoot regeneration.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Austin, S., Cassells, A. C.: Variation between plants regenerated from individual calli produced from separated potato stem callus cells. - Plant Sci. Lett.31:107–114, 1983.

    Article  Google Scholar 

  • Binding, H., Nehls, R., Schieder, O., Sopory, S. K., Wenzel, G.: Regeneration of mesophyll protoplasts isolated from dihaploid clones ofSolanum tuberosum. - Physiol. Plant.43: 52–54, 1978.

    Article  Google Scholar 

  • Bokelmann, G. S., Roest, S.: Plant regeneration from protoplasts of potato(Solanum tuberosum cv. Bintje). - Z. Pflanzenphysiol.109: 259–265, 1983.

    CAS  Google Scholar 

  • Carlberg, I., Karlsson, S., Eriksson, T.: Improved culture techniques for potato protoplasts. - In: Bajaj, Y. P. S. (ed.): Potato. Biotechnology in Agriculture and Forestry. Vol. 3. Pp. 187–194. Springer-Verlag, Berlin-Heidelberg-New York-London-Paris-Tokyo 1987.

    Google Scholar 

  • Foulger, D., Jones, M. G. K.: Improved efficiency of genotype-dependent regeneration from protoplasts of important potato cultivars. - Plant Cell Rep.5: 72–76, 1986.

    Article  Google Scholar 

  • Grun, P., Wang, M.-W., Radke, S.: Regeneration of plants from potato protoplasts. - In: Bajaj, Y. P. S. (ed.): Potato. Biotechnology in Agriculture and Forestry. Vol. 3. Pp. 195–210. Springer-Verlag, Berlin-Heidelberg-New York-London-Paris-Tokyo 1987.

    Google Scholar 

  • Haberlach, G. T., Cohen, B. A., Reichert, N. A., Baer, M. A., Towill, L. E., Helgeson, J. P.: Isolation, culture and regeneration of protoplasts from potato and several relatedSolanum species. - Plant Sci.39: 67–74, 1985.

    Article  Google Scholar 

  • Karp, A., Nelson, R. S., Thomas, E., Bright, S. W. J.: Chromosome variation in protoplast-derived potato plants. - Theor. appl. Genet.63: 265–272, 1982.

    Article  Google Scholar 

  • Kikuta, Y., Fujino, K., Saito, W., Okazawa, Y.: Viability, DNA synthesis and cell wall regeneration on potato protoplasts. - In: Bajaj, Y. P. S. (ed.): Potato. Biotechnology in Agriculture and Forestry. Vol. 3. Pp. 177–186. Springer-Verlag, Berlin-Heidelberg-New York-London-Paris-Tokyo 1987.

    Google Scholar 

  • Menczel, L., Nagy, F., Kiss, Zs. R., Maliga, P.: Streptomycin resistant and sensitive somatic hybrids ofNicotiana tabacum + Nicotiana knightiana: correlation of resistance to N.tabacum plastids. -Theor. appl. Genet.59: 191–195, 1981.

    Article  CAS  Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. - Physiol. Plant.15: 473–497, 1962.

    Article  CAS  Google Scholar 

  • Schenk, R. U., Hildebrandt, A. C.: Medium and techniques for induction and growth of monocotyledon- ous and dicotyledonous plant cell cultures. - Can. J. Bot.50:199–204, 1972.

    Article  CAS  Google Scholar 

  • Schumann, U., Koblitz, H.: Studies on plant recovery from mesophyll protoplasts ofSolanum tuberosum L. andSolanum phureja Juz. et Buk. - Biol. Plant.25:180–186, 1983.

    Article  Google Scholar 

  • Shepard, J. F.: Cultivar dependent cultural refinements in potato protoplast regeneration. - Plant Sci. Lett.26:127–132, 1982.

    Article  Google Scholar 

  • Shepard, J. F., Totten, R. E.: Mesophyll cell protoplasts of potato. Isolation, proliferation, and plant regeneration. -Plant Physiol.60: 313–316, 1977.

    Article  PubMed  CAS  Google Scholar 

  • Sidorov, V. A., Piven, N. Sh., Gleba, Yu., Sytnik, K. M.: Somaticheskaya Gibridizatsiya Paslenovykh. [Somatic Hybridization of Solanaceae Species.-Naukova Dumka, Kiev 1985.

  • Sidorov, V. A., Zubko, M. K., Kuchko, A. A., Komarnitsky, I. K., Gleba, Y. Y.: Somatic hybridization in potato: use of γ-irradiated protoplasts ofSolanum pinnatisectum in genetic reconstruction. -Theor. appl. Genet.74: 364–368, 1987.

    Article  Google Scholar 

  • Tavazza, R., Ancora, G.: Plant regeneration from mesophyll protoplasts in commercial potato cultivars (Primura, Kennebec, Spunta, Desirée). - Plant Cell Rep.5: 243–246, 1986.

    Article  CAS  Google Scholar 

  • Taylor, R. J., Secor, G. A.: A shoot induction procedure altered for increased shooting efficiency of potato protoplasts-derived calli (cvs. Crystal and Norgold Russet). - Potato Res.31:651–658, 1988.

    Article  Google Scholar 

  • Upadhya, M. D.: Isolation and culture of mesophyll protoplasts of potato(Solanum tuberosum L.). - Potato Res.18: 438–445, 1975.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Plant Molecular Biology, Czechoslovak Academy of Sciences, Na sádkách 702, 370 05, České Budějovice, Czechoslovakia

    J. Bříza & Iveta Machová

Authors
  1. J. Bříza
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Iveta Machová
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bříza, J., Machová, I. Regeneration of plants from leaf mesophyll protoplasts of the tetraploid potato cultivars Xenia and Bintje. Biol Plant 33, 225–233 (1991). https://doi.org/10.1007/BF02897888

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02897888

Keywords

Search

Navigation