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Studies on plant recovery from mesophyll protoplasts ofSolatium tuberosum L. andSolanum phureja Juj. et Buk

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Biologia Plantarum

Abstract

Plants were regenerated from mesophyll protoplasts of axenically growing plants of four dihaploid clones and five tetraploid cultivars ofSolanum tuberosum L. andSolanum phureja Juz. etBuk.

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Abbreviations

MS:

Murashige and Skoog

NAA:

1-naphthaleneacetio acid

2,4-D:

2,4-dichlorophenoxyacetic acid

BA:

6-benzyladenine

2iP:

N6-(Δ2-isopentenyl)adenine

GA3 :

gibberellic acid

ABA:

abscisic acid

MES:

2-N-morpholinoethane sulfonic acid

References

  • Binding, H., Schieder, O., Sopory, S. K., Wenzel, G.: Regeneration of mesophyll protoplasts isolated from dihaploid clones ofSolanum tuberosum. - Physiol. Plant.48: 52 - 54, 1978.

    Article  Google Scholar 

  • Butenko, R. G., Kuchko, A. A., Vitenko, V. A.: Isolation, culture, and plant regeneration studies on protoplasts from two potato species. - Proc. Int. Conf. Regulation Developmental Processes in Plants (Abstr.) 111, Halle 1977.

  • Evans, P. K., Keates, A. G., Cocking, E. C.: Isolation of protoplasts from cereal leaves. -Planta104: 178–181, 1972.

    Article  CAS  Google Scholar 

  • Gamborg, O. L., Miller, R. A., Ojima, K.: Nutrient requirements of suspension cultures of soybean root cells. - Exp. Cell Res.50: 151 - 158, 1968.

    Article  PubMed  CAS  Google Scholar 

  • Gunn, R. F., Shepard, J. F.: Regeneration of plants from mesophyll-derived protoplasts of British potato (S. tuberosum L.) cultivars. - Plant Sci. Lett.22: 97–101, 1981.

    Article  Google Scholar 

  • Koblitz, H., Hirte, W. F.: Einsatz eines Enzympräparats ausPenicillium citreo-viride bei der Isolierung von Protoplasten aus pflanzlichen Gewebekulturen. - Biochem. Physiol. Pflanzen170: 295–300, 1976.

    CAS  Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bio assays with tobacco tissue cultures. -Physiol. Plant.15: 473–497, 1962.

    Article  CAS  Google Scholar 

  • Opatrný, Z., Schumann, TJ., Rakouský, S., Koblitz, H.: The role of some exogenous and endogenous factors in the isolation of protoplasts from potato cell cultures and their recovery in the cell colonies. - Biol. Plant.22: 107–116, 1980.

    Article  Google Scholar 

  • Schumann, U., Koblitz, H., Opatrny, Z.: Plant recovery from long-term callus cultures and from suspension culture derived protoplasts ofSolanum phureja. - Biochem. Physiol. Pflanzen175: 670–675, 1980.

    Google Scholar 

  • Shepard, J. F., Bidney, D., Shanin, E.: Potato protoplasts in crop improvement. - Science208: 17–24, 1980.

    Article  PubMed  Google Scholar 

  • Shepard, J. F., Totten, R. E.: Mesophyll cell protoplasts of potato (Solanum tuberosum L.). Isolation, proliferation, and plant regeneration. - Plant Physiol.60: 313 - 316, 1977.

    Article  PubMed  CAS  Google Scholar 

  • Thomas, E.: Plant regeneration from shoot culture-derived protoplasts of tetraploid potato (Solanum luberosum cv. Maris Bard).- Plant Sci. Lett.23: 81–88, 1981.

    Article  Google Scholar 

  • Wenzel, G., Schieder, O., Przewozny, T., Sopory, S. K., Melchers, G.: Comparison of single cell culture derivedSolanum tuberosum L. plants and a model for their application in breeding programs. - Theor. appl. Genet.55: 49–55, 1979.

    Article  Google Scholar 

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Authors and Affiliations

  1. Zentralinstitut für Genetik und Kulturpflanzenforschung der Akademie der Wissenschaften der DDR, DDR-4325, Gatersleben, Corrensstrasse 3

    Ursula Schumann & H. Koblitz

Authors
  1. Ursula Schumann
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  2. H. Koblitz
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Schumann, U., Koblitz, H. Studies on plant recovery from mesophyll protoplasts ofSolatium tuberosum L. andSolanum phureja Juj. et Buk. Biol Plant 25, 180–186 (1983). https://doi.org/10.1007/BF02902099

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  • DOI: https://doi.org/10.1007/BF02902099

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