Abstract
Simultaneous uptake of NH+ 4, Na+, Mg2+, Ca2+, NO- 3, SO2- 4, (NO- 2), H2PO- 4 and C1- ions by N-limited winter wheat seedlings(Triticum aestivum L., cv. Regina) in a single depletion experiment was investigated. Individual ion species in uptake solution samples were determined using capillary isotachophoresis. The operating systems used are described in detail. Processing of obtained concentration data allow to construct time curves of individual ions concentration in uptake solution, time curves of accumulative uptake, and curves for uptake rate versus time and uptake rate versus external concentration. From these curves there is possible to reveal a more complex picture of behaviour of individual ions during uptake process as well as to assess interactions among them. Attention was paid to the points of intersection of time curves of NO- 3 and NH+ 4 uptake rates. These points characterized by equal uptake rates may be considered as limits for preference of given ion species under given experimental conditions.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aslam, M., Huffaker, R. C., Rains, D. W.: Early effects of salinity on nitrate assimilation in barley seedlings. - Plant Physiol.76: 321–325, 1984.
Bloom, A. J., Finazzo, J.: The influence of ammonium and chloride on potassium and nitrate absorption by barley roots depends on time of exposure and cultivar. - Plant Physiol.81: 67–69, 1986.
Bošek, P., Miedziak, I., Deml, M., Janák, J.: Use of complex formation equilibria in the analytical isotachophoresis of strong electrolyte ions. - J. Chromatogr.137: 83–91, 1977.
Cheam, V., Chau, A. S. Y.: Automated simultaneous analysis of anions and monovalent and divalent cations. - Analyst112: 993–997, 1987.
Clarkson, D. T., Warner, A. J.: Relationship between root temperature and the transport of ammonium and nitrate ions by Italian and perennial ryegrass(Lolium multiflorum andLolium perenne). - Plant Physiol.64: 557–561, 1979.
Dambroth, M., El Bassam, N..: Genotypic variation in plant productivity and consequence for breeding of “low input cultivars”. - In: Third International Symposium on Genetic Aspects of Plant Nutrition., Braunschweig1988.
Goyal, S. S., Huffaker, R. C.: A novel approach and a fully automated microcomputer-based system to study kinetics of NO- 3, NO- 3 and NH+ 4 transport simultaneously by intact wheat seedlings. - Plant Cell Environ.9: 209–215, 1986a.
Goyal, S. S., Huffaker, R. C.: The uptake of NO- 3, NO- 2 and NH- 4 by intact wheat(Triticum aestivum) seedlings. - Plant Physiol.82: 1051–1056, 1986b.
Ingemarsson, B., Oscarson, P., Ugglas, M., Larsson, C. M.: Nitrogen utilization inLemna. III. Short-term effects of ammonium on nitrate reduction. - Plant Physiol.85: 865–867, 1987.
Jensén, P., Perby, H.: Growth and accumulation of N, K+, Ca2+ and Mg2+ in barley exposed to various nutrient regimes and root/shoot temperatures. - Physiol. Plant.67: 159–165, 1986.
Jensén, P., Erdei, L., Móller, I. M.: K+ uptake in plants roots: Experimental approach and influx models. - Physiol. Plant.70: 743–748, 1987.
Klimashevsky, E. L.: Physiology of genetic differences of plants in mineral nutrition. - In: Third International Symposium on Genetic Aspects of Plant Nutrition. Braunschweig 1988.
Matejovic, I., Polonsky, J.: Capillary isotachophoresis in the study of absorption of inorganic ions by roots of intact plants. - J. Chromatogr.438: 454–458, 1988.
Minotti, P. I., Williams, D. C., Jackson, W. A.: Nitrate uptake by wheat as influenced by ammonium and other cations. - Crop Sci.9: 9–14, 1969.
Morgan, M. A., Jackson, W. A.: Suppression of ammonium uptake by nitrogen supply and its relief during nitrogen limitation. - Physiol. Plant.73: 38–45, 1988.
Perby, H., Jensén, P.: Varietal differences in uptake and utilization of nitrogen and other macro-elements in seedlings of barley,Hordeum vulgare. - Physiol. Plant.58: 223–230, 1983.
Perby, H., Jensén,.P: Variation in growth and accumulation of N, K+, Ca2+ and Mg2+ among barley cultivars exposed to various nutrient regimes and root/shoot temperatures. - Physiol. Plant.67: 166–172, 1986.
Ralston, A.: Základy Numerické Matematiky. [A First Course in Numerical Analysis.] - Academia, Praha 1978.
Siddiqi, M. Y., Glass, A. D. M.: Studies of growth and mineral nutrition of barley varieties. I. Effect of potassium supply on the uptake of potassium and growth. - Can. J. Bot.61: 671–678, 1983.
Siddiqi, M. Y., Glass, A. D. M.: The influence of monovalent cations upon influx and efflux of Ca2+ in barley roots. -Plant Sci. Lett.23: 103–114, 1984.
Woodend, J. J., Glass, A. D. M., Person, C. O.: Intraspecific variation for nitrate uptake and nitrogen utilization in wheat(T. aestivum L.) grown under nitrogen stress. - J. Plant Nutr.9:1213–1225, 1986.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Matejovič, I. Simultaneous Study of NH+ 4, Na+, Mg2+, Ca2+, NO2- 3, SO2- 4, (NO- 2), H{2in}PO- 4 and CI- Uptake by Intact Winter Wheat Seedlings in a Single Depletion ExperimentPO- 4 and CI- Uptake by Intact Winter Wheat Seedlings in a Single Depletion Experiment. Biol Plant 33, 366–376 (1991). https://doi.org/10.1007/BF02897688
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02897688