Abstract
Changes in the activity and localization of nonspecific esterase, acid phosphatase, α-galactosidase and β-glucosidase inL. regale pistils after pollination with μ-irradiated pollen were studied. In the embryo sac and in the ovule reduction of AS-esterase and α-galactosidase and, at the same time, enhancement of α-esterase, acid phosphatase and β-glucosidase activities were observed. The changes in hydrolytic enzyme activities are discussed as manifestations of lethal factors resulting from structural disturbances of DNA in the generative nucleus and in sperms caused by irradiation.
Similar content being viewed by others
References
Beneš, K.: [Histochemical demonstration of esterase in the root tip ofVicia faba L. with azocoupling method.] In Russ. -Biol. Plant.4: 211–219, 1962.
Beneš, K., Georgieva, I. D., Poláčková, D.: The presence and distribution of β-glucosidase in root tip. - Biol. Plant.15: 88–94, 1973.
Calgari, P., Ingram, N. R., Yinks, I. L.: Gene transfer inNicotiana rustica by means of irradiated pollen. I. Unselected progenies. - Heredity47: 17–26, 1981.
Cave, M. S., Brown, S. W.: The detection and nature of dominant lethals inLilium. II. Cytological abnormalities in ovules after pollen irradiation. - Amer. J. Bot.41: 69–483, 1954.
Fawcett, D. W.: The Cell. - Saunders Co., Philadelphia 1981.
Georgieva, I. D.: [Histochemical study on the localization of some hydrolytic enzymes duringLilium regale Wils. embryonic development.] In Bulg. - Genet. Selek. (Sofia)11: 201–205, 1977.
Georgieva, I. D.: Cytochemical investigation ofLilium regale embryogenesis after pollination with μ-irradiated pollen. -In: Proceedings of 8th International Symposium on Sexual Reproduction in Seed Plants, Ferns and Moses. Pp. 167. Pudoc, Wageningen 1984.
Georgieva, I. D.: Histochemical investigation of peroxidase during the embryogenesis ofLilium regale Wils. following pollination with μ-irradiated pollen. - In: Journées Internationales d’Etude du Group Polyphénols. Université des Sciences et Techniques du Languedoc, Montpellier. P. 72, 1987.
Grant, J. E., Pandey, K. K., Williams, E. G.: Pollen nuclei after ionising irradiation for egg transformation inNicotiana. - New Zeal. J. Bot.18: 339–341, 1980.
Hanson, K. P., Komar, P. E.: Molekulyarnye mekhanizmy radiatsionnoi gibeli kletok. [Molecular mechanisms of radiation death of cells.] In Russ. - Ënergoatomizdat, Moskva 1985.
Jones, R. P.: Measures of yeast death and deactivation and their meaning. Part. 1. - Process Biochem.22: 117–128, 1987.
Lojda, Z., Gossrau, R., Schiebler, T. H.: Enzyme Histochemistry. - Springer Verlag, Berlin-Heidelberg-New York 1979.
Lojda, Z., Papoušek, F.: Základy Histochemického Průkazu Enzymů. [Bases of Histochemical Enzyme Test.] In Czech. - Ústav pro další vzdělávání středních zdravotnických pracovníků, Brno 1970.
Malec, J., Przybyszewski, W. M., Grabarczyk, E. S., Czartoryska, B.: Mechanism of unbalanced growth-induced cell damage. 1. A probable role for hydrolytic enzymes synthesis. - Chem. biol. Interactions57: 315–324, 1986.
Matile, Ph.: The Lytic Compartment of Plant Cells. - Springer-Verlag, Wien 1975.
Price, P. B.: Reproduction and growth in various lily species following X- and gamma-irradiation of pollen and X-irradiation of seeds.- Dissert. Abstr.17: 1650–1651, 1957.
Vassileva-Dryanovska, O. A.: The induction of haploid embryos and tetraploid endosperm nuclei with irradiated pollen inLilium. -Hereditas55: 160–165, 1966.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Georgieva, I.D. The activity and localization of some hydrolytic enzymes during early embryogenesis oflilium regale after pollination with μ-frradiated pollen. Biol Plant 33, 337–344 (1991). https://doi.org/10.1007/BF02897684
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02897684