Abstract
Organogenic callus cultures ofPapaver somniferum L. were studied with the aim of describing the morphology of the callus and observing the changes occurring during differentiation and induction of organogenesis. The morphology of the cells and the formation of meristemoid areas were studied on a light microscopic level. Histological evidence showed that from the inner meristematic centres, protracheal elements are differentiated, from the surface meristematic centres meristemoids are formed and therefrom green leaf-like organoids.
Similar content being viewed by others
References
Brezeanu, A., Davis, D. G.: Successive changes in the ultrastructure ofOryza sativa L. cells during growth and aging. - Protoplasma98: 163–179, 1979.
Erdelský, K.: Wachstumscharakteristik einer Callus Gewebekultur vonPapaver somniferum L. - Acta Fac. Rer. nat. Univ. Comen. - Physiol. Plant.111: 1–10. 1971.
Frolova, L.: [Peculiarity of the cultivated plant cells.] In Russ. - In:Butenko, R. G. (ed.): Kultura Kletok Rastenii. [Plant Cell Culture.] Pp. 5–6. Nauka, Moskva 1981.
Gamborg. O. L. Constabel, F., Shyluk, J. P.: Organogenesis in callus from shoot apices ofPisum sativum. Physiol. Plant.30: 125–128, 1974.
Howarth, M. J., Peterson, R. L., Tomes, D. T., Swanson, E. B.: Ultrastructural characteristics of an achiorophyllous callus line ofLotus corniculatus. - In vitro17: 891–900, 1981.
Howarth, M. J., Peterson, R. L., Tomes, D. T.: Cellular differentiation in small clumps ofLotus corniculatus callus. - Can. J. Bot.61: 507–517, 1983.
Kutchan, T. M., Ayabe, S., Kruger, R. J., Coscia, E. M., Coscia, C. J.: Cytodifferentiation and alkaloid accumulation in cells ofPapaver bracteatum. - Plant Cell Rep.2: 281–284, 1983.
Lux, A.: [A rapid method for staining semithin sections of plant material.] In Slovak. - Biológia (Bratislava)36: 753–757, 1981.
Maheswaran, G., Wiliams, E. G.: Origin and development of somatic embryoids formed directly on immature embryos ofTrifolium repens in vitro. - Ann. Bot.56: 619–630, 1985.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. -Physiol. Plant.15: 473–497, 1962.
Nessler, C. L., Mahlberg, P. B.: Ultrastructure of laticifers redifferentiated organs on callus fromPapaver somniferum (Papaveraceae). - Can. J. Bot.57: 675–685, 1979.
Ross, M. K., Thorpe, T. A., Costerton, J. W.: Ultrastructural aspects of shoot initiation in tobacco callus culture. - Amer. J. Bot.60: 788–795, 1973.
Šamaj, J., Bobák, M., Erdelský, K.: [Plastids in cells ofPapaver somniferum L. organogenic calli grown on various media.] In Slovak. - Biológia (Bratislava)43: 577–588, 1988.
Stefaniak, B., Woźny, A.: Investigations on the ultrastructure of the callus tissue ofLiriodendron tulipifera L. developedin vitro. - Acta Soc. Bot. Pol.52: 3–8, 1983.
Thorpe, T. A., Meier, D. D.: Starch metabolism, respiration and shoot formation in tobacco callus cultures. -.J. exp. Bot.27: 365–369. 1972.
Thorpe, T. A., Meier, D. D.: Starch metabolism in shoot-forming tobacco callus. - J. exp. Bot.25: 288–294, 1974.
Thorpe, T. A., Murashige, T.: Starch accumulation in shoot-forming tobacco callus cultures. - Science160:421–422, 1968.
Orrey, J.: The initiation of organized development in plants. - Adv. Morphol.5: 39–91, 1966.
Vasil, V., Hildebrandt, A. C.: Differentiation of tobacco plants from single, isolated cells in microcultures. -Science150: 890–892, 1965.
Wilson, H. J., Israel, H. W., Steward, F. C.: Morphogenesis and the fine structure of cultured cells. - Science15: 57–73, 1974.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Šamaj, J., Bobák, M. & Erdelský, K. Histological-anatomical studies of the structure of the organogenic callus inPapaver somniferum L.. Biol Plant 32, 14–18 (1990). https://doi.org/10.1007/BF02897337
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02897337