Skip to main content
SpringerLink
Log in

Cytodifferentiation and alkaloid accumulation in cultured cells of Papaver bracteatum

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Cultured Papaver bracteatum cells accumulate large amounts of dopamine (0.1–4 mg/g fresh wt.) and much lower levels of the alkaloids, thebaine (0–6 μg/g fresh wt.) and sanguinarine (10–500 μg/g fresh wt.). Upon removal of hormone from their culture media, organogenesis and an elevation in morphinan alkaloid concentration were observed. Light and electron microscopic studies reveal that a correlation exists between the appearance of laticifer-like cells and thebaine in these cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BA:

6-Benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

IBA:

indole-3-butyric acid

References

  • Deus B, Zenk MH (1982) Biotechnology and Bioengineering 24: 1965–1974

    Google Scholar 

  • Dickenson PB, Fairbairn JW (1975) Ann. Bot. 39: 707–712

    Google Scholar 

  • Furuya T, Ikuta A, Syono K (1972) Phytochem. 11: 3041–3044

    Google Scholar 

  • Hodges CC, Rapoport H (1982) J. Natural Prod. 45: 481–485

    Google Scholar 

  • Kamimura S, Akutsu M (1976) Agr. Biol. Chem. 40: 899–906

    Google Scholar 

  • Kamimura S, Akutsu M, Nishikawa M (1976) Agr. Biol. Chem. 40: 913–919

    Google Scholar 

  • Kamimura S, Nishikawa M (1976) Agr. Biol. Chem. 40: 907–911

    Google Scholar 

  • Kamo KK, Kimoto W, Hsu AF, Mahlberg PG, Bills DD (1982) Phytochem. 21: 219–222

    Google Scholar 

  • Lockwood GB (1981) Phytochem. 20: 1463–1464

    Google Scholar 

  • Morris P, Fowler MS (1980) Planta Med. 39: 284–285

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol. Plant 15: 473–497

    Google Scholar 

  • Neumann D, Krauss G, Hieke M, Gröger D (1983) Planta Med. 48: 20–23

    Google Scholar 

  • Nessler CL (1982) Physiol. Plant. 55, 453–458

    Google Scholar 

  • Nessler CL, Mahlberg PG (1979) Can. J. Bot. 57: 675–685

    Google Scholar 

  • Nessler CL, Mahlberg PG (1977) Amer. J. Bot. 64: 541–551

    Google Scholar 

  • Roberts MF, McCarthy D, Kutchan TM, Coscia CJ (1983) Arch. Biochem. Biophys. 222: 599–609

    Google Scholar 

  • Schuchmann R, Wellmann E (1983) Plant Cell Reports 2: 88–91

    Google Scholar 

  • Schulze CH, Schnepe E, Mothes K (1967) Flora Abt. A 158: 458–460

    Google Scholar 

  • Staba EJ, Zito S, Amin M (1982) J. Natural Prod. 45: 256–262

    Google Scholar 

  • Tam WHJ, Constabel F, Kurz WGW (1980) Phytochem. 19: 486–487

    Google Scholar 

  • Thureson-Klein A (1970) Ann. Bot. 34: 751–761

    Google Scholar 

  • Yoshikawa T, Furuya T (1982) In: Fujiwara A (ed) Proc. 5th Internat. Congress of Plant Tissue and Cell Culture, Abe Printing Ltd., Tokyo, Japan, pp. 307–308

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. E. A. Doisy Department of Biochemistry, Saint Louis University School of Medicine, 63104, St. Louis, MO, USA

    T. M. Kutchan, S. Ayabe, E. M. Coscia & C. J. Coscia

  2. School of Pharmacy, Ferris State College, 49307, Big Rapids, MI, USA

    R. J. Krueger

Authors
  1. T. M. Kutchan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Ayabe
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. J. Krueger
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. M. Coscia
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. C. J. Coscia
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by F. Constabel

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kutchan, T.M., Ayabe, S., Krueger, R.J. et al. Cytodifferentiation and alkaloid accumulation in cultured cells of Papaver bracteatum . Plant Cell Reports 2, 281–284 (1983). https://doi.org/10.1007/BF00270181

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00270181

Keywords

Search

Navigation