Abstract
Morphology of pollen grains collected from healthy and virus infected plants ofChenopodium quinoa L.,Chenopodium album L. andNicotiana tabacum L. cv. Samsun was investigated using scanning electron microscopy (SEM). Pollen grains from tobacco plans infected with tobacco mosaic virus (TMV) were smaller, with rounded shape and conspicuous deformation of aperture unlike oval and smooth pollen grains from healthy plants. No morphological alterations were observed inC. quinoa andC. album plants infected with TMV and cucumber mosaic virus (CMV). Polyacrylamide gel electrophoresis of pollen proteins revealed substantial quantitative and qualitative differences in protein components of pollen grains collected from healthy and virus infected plants
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Abbreviations
- CMV:
-
cucumber mosaic virus
- TMV:
-
tobacco mosaic virus
References
Baldo, B.A., Bass, D.J.: Standardization of grass pollen allergens: the spectra of pollen allergens from six common grasses. - Rev. Paleobot. Palynol.64: 87–95, 1990.
Brčák, J.: [Strain of cucumber mosaic virus from Křimice (CMV-Ke)]. - Ochrana Rost. (Praha)15: 81–88, 1979. [In Czech.]
Erdtmann, G.: The acetolysis method. A revised description. - Svensk. bot. Tidskrift54: 561–564, 1960.
Francki, R.I.B., Miles, R.: Mechanical transmission of sowbane mosaic virus carried on pollen from infected plants. -Plant Pathol.34: 11–19, 1985.
Haight, E., Gibbs, A.: Effect of viruses on pollen morphology. - Plant Pathol.32: 369–372, 1983.
Hamilton, R.I., Leung, E., Nichols, C.: Surface contamination of pollen by plant viruses. Phytopathology67: 395–399, 1977.
Knox, R.B., Heslop-Harrison, J.: Pollen-wall proteins: localisation of antigenic and allergenic proteins in the pollen-grain walls ofAmbrosia spp. (ragweeds). - Cytobiosis4: 49–54, 1971.
Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. - Nature227: 680–685, 1970.
Mink, G.I.: Pollen- and seed-transmitted viruses and viroids. - Annu. Rev. Phytopathol.31: 375–402, 1993.
Negrini, A.C.: Pollens as allergens. - Aerobiologia8: 9–15, 1992.
Stace-Smith, R., Hamilton, R.I.: Inoculum thresholds of seedborne pathogens. Viruses. Phytopathology78: 875–880, 1987.
Von Wahl, P.G., Kersten, W.:Fusarium andDidymella - neglected spores in the air. - Aerobiologia7: 111–117, 1991.
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Acknowledgement: This research was granted as the project Z 209/3 by the Ministry of Health, Czech Republic.
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Petrzik, K., Špak, J., Nebesářová, J. et al. The effect of virus infection on morphology and protein components of pollen grains. Biol Plant 38, 445–450 (1996). https://doi.org/10.1007/BF02896678
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DOI: https://doi.org/10.1007/BF02896678