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Ultrastructural modifications of mitochondria and rough endoplasmic reticulum of liver and spleen after a period of hypoxia

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Virchows Archiv B

Summary

Hypoxia (5% oxygen in nitrogen at normal atmospheric pressure) produces rapid changes in the ultrastructural stability of mitochondria of spleen and liver and a narrowing of the clefts of the rough endoplasmic reticulum (ergastoplasm) of hepatocytes.

These changes are reversible. One hour after the end of a 15–20 minutes hypoxic period, the endoplasmic reticulum is, on the contrary, widened and part of the ribosomes are gathered in clusters separate from the membranes.

It is suggested that these modifications are linked with a temporary decrease in protein synthesis. A comparison is made with other ultrastructural changes produced in the liver and spleen, notably by sulfur containing radioprotective substances.

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References

  • Arakawa, K., Jezequel, A. M., Macvie, S. I., Johnston, R., Perz, Z. M., Steiner, J. W.: The liver murine transplantation (runt) disease. Observations of the acute lesions by light and electron microscopy. Amer. J. Path.49, 257–300 (1966).

    PubMed  CAS  Google Scholar 

  • Ashworth, C. T., Werner, D. J., Glass, M. D., Arnold, N. J.: Spectrums of fine structural changes in hepatocellular injury due to thioacetamide. Amer. J. Path.47, 917–951 (1965).

    PubMed  CAS  Google Scholar 

  • Bassi, H., Bernelli-Zazzera, A.: Ultrastructural cytoplasmic changes of liver cells after reversible and irreversible ischemia. Exp. molec. Path.3, 332–350 (1964).

    Article  Google Scholar 

  • Beaumariage, M. L., Caneghem, P. van, Plomteux, G., Bacq, Z. M., Heusghem, C.: Accroissement du taux sanguin de différents enzymes cellulaires après radioprotecteurs soufrés. 4ème Congrès International de Radiobiologie, Evian, 1970.

  • Betz, E. H., Lelièvre, P., Smoliar, V.: Protective effectiveness of some sulfur containing substances and oxygen uptake in the rat. Int. J. Radiat. Biol.12, 163–168 (1967).

    Article  CAS  Google Scholar 

  • Braun, H., Koch, R.: Untersuchungen über einen biologischen Strahlenschutz 86. Veränderungen der Mitochondrien nach Strahlenschutzenden Sulfhydrylkörpern bzw. nicht schützenden Homologen. Strahlentherapie135, 628–631 (1968).

    PubMed  CAS  Google Scholar 

  • Bucher, N.: Regeneration of mammalian liver. Int. Rev. Cytol.15, 245–300 (1963).

    PubMed  CAS  Google Scholar 

  • Fawcett, D. W.: Observations on the cytology and electron microscopy of hepatic cells. J. nat. Cancer Inst.15, 1475–1503 (1955).

    PubMed  CAS  Google Scholar 

  • Firket, H., Lelièvre, P.: Effet de la cystamine sur la respiration, la phosphorylation oxydative et l’ultrastructure des mitochondries du rat. Int. J. Radiat. Biol.10, 403–415 (1966).

    Article  CAS  Google Scholar 

  • Firkin, F. C., Linnane, A. W.: Biogenesis of mitochondria. The effect of chloramphenicol on regenerating rat liver. Exp. Cell Res.55, 68–76 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Huebner, G., Bernhard, W.: Das submikroskopische Bild der Leberzelle nach temporärer Durchblutungssperre. Beitr. path. Anat.125, 1–29 (1961).

    Article  CAS  Google Scholar 

  • Hugon, J., Maisin, J., Borgers, M.: Modifications ultrastructurales après radioprotecteurs. Int. J. Radiat. Biol.11, 105–116 (1966).

    Article  CAS  Google Scholar 

  • Lelièvre, P., Smoliar, V., Betz, E. H.: Influence de certains radioprotecteurs soufrés sur la tension d’oxygène dans la rate du rat. Atomkernenergie, p. 445–447. Stuttgart: Thieme 1969.

    Google Scholar 

  • Mölbert, E., Guerritore, D.: Elektronenmikroskopische Untersuchungen am Leberparenchyme bei akuter Hypoxie. Beitr. path. Anat.117, 33–49 (1957).

    Google Scholar 

  • Oudea, P. R.: Anoxic changes of liver cells; electron microscopic study after injection of colloidal mercury. Lab. Invest.12, 386–394 (1963).

    PubMed  CAS  Google Scholar 

  • Steiner, J. W., Baglio, C. N.: Electron microscopy of the cytoplasm of parenchymal liver cells in α-naphthylisothiocyamate-induced cirrhosis. Lab. Invest.12, 765–790 (1963).

    PubMed  CAS  Google Scholar 

  • Stenram, U., Willen, R.: The effect of actinomycin D on ultrastructure and radioautographic ribonucleic acid and protein labelling in rat liver after partial hepatectomy. Cancer Res.26, 765–772 (1966).

    PubMed  CAS  Google Scholar 

  • Sulkin, N. M., Sulkin, D.: An electron microscope study of the effects of chronic hypoxia on cardiac mucle, hepatic and autonomic ganglion cells. Lab. Invest.14, 1523–1547 (1965).

    PubMed  CAS  Google Scholar 

  • Thorpe, M. E. C., Shorey, C. D.: Long-term alcohol administration. Its effects on ultrastructure and lipid content of the rat liver cell. Amer. J. Path.48, 557–578 (1966).

    PubMed  CAS  Google Scholar 

  • Viragh, S., Bartok, I.: An electron microscopic study of the regeneration of the liver following partial hepatectomy. Amer. J. Path.49, 825–845 (1966).

    PubMed  CAS  Google Scholar 

  • Weibel, E. R.: Stereological principles for morphometry in electron microscopic cytology. Int. Rev. Cytol.26, 235–302 (1969).

    PubMed  CAS  Google Scholar 

  • — Stäubli, W., Gnägi, H. R., Hess, F.: Correlated morphometric and biochemical studies on the liver cells. I. Morphometric model, stereologic methods, and normal morphometric data for rat liver. J. Cell Biol.42, 68–91 (1969).

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratoires d’Anatomie Pathologique et de Pathologie Générale, Université de Liège, Belgium

    H. Firket & M. L. Beaumariage

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  1. H. Firket
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  2. M. L. Beaumariage
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Firket, H., Beaumariage, M.L. Ultrastructural modifications of mitochondria and rough endoplasmic reticulum of liver and spleen after a period of hypoxia. Virchows Arch. Abt. B Zellpath. 8, 342–349 (1971). https://doi.org/10.1007/BF02893543

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  • DOI: https://doi.org/10.1007/BF02893543

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