Abstract
Phaseolus coccineus storage globulins were extracted from mature cotyledons, purified and characterized. Three major proteins were separated. A component showing erythroagglutinating activity was thoroughly purified by thyroglobulin-Sepharose chromatography. The relative molecular masses of the three fractions are Mr = 330, 178, and 500 kDa as determined by polyacrylamide gel electrophoresis (PAGE). They correspond to the proteins found in other systems and classified as phytohaemagglutinin (PHA), vicilin and legumin, respectively. Electrophoretic analyses under denaturating conditions (SDS-PAGE) evidenced the major subunits for the three proteins. Isoelectrofocusing of the isolated proteins indicated a large heterogeneity for vicilin.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Badley, A. R., Atkinson, D., Hauser, H., Oldani, D., Green, J. P., Stubbs, J. M.: The structure, physical and chemical properties of the soybean protein glycinin. - Biochim. Biophys. Acta412:214–228, 1975.
Bollini, R., Chrispeels, M. J.: Characterization and subcellular localization of vicilin and phytohemagglutinin the two major reserve proteins ofPhaseolus vulgaris L. - Planta142:291–298, 1978.
Bollini, R., Chrispeels, M. J.: The rough endoplasmic reticulum is the site of reserve-protein synthesis in the developingPhaseolus vulgaris cotyledons. - Planta146:487–501, 1979.
Brown, J. W. S., Bliss, F. A., Hall, T. C.: Microheterogeneity of G1 storage protein from French bean with isoelectrofocusing. - Plant Physiol.66:838–840, 1980.
Croy, R. R. D., Derbyshire, E., Krisand, T. G., Boulter, D.: Legumin inPisum sativum andVicia faba. - New Phytol.83:29–35, 1986.
Danielsson, C. E.: Seed globulins of the gramineae and leguminosae. - Biochem. J.44:387–400, 1949.
Davis, B. J.: Disc electrophoresis - II Method and application to human serum proteins.- Ann. N. Y. Acad. Sci.121:404–427, 1964.
Derbyshire, E., Wright, D. J., Boulter, D.: Legumin and vicilin, storage proteins of legume seeds.- Phytochemistry15:3–24, 1976.
Durante, M., Covi, A., Barsanti, L.: Microsomal rat liver B-glucuronidase: characterization and behaviour. - Ital. J. Biochem.23:292–305, 1974.
Ericson, M. C, Chrispeels, M. J.: Isolation and characterization of glucosamine-containing storage glycoproteins from the cotyledons ofPhaseolus aureus. - Plant Physiol.52:98–104, 1973.
Felsted, R. D., Leavitt, R. D., Bachur, N. R.: Purification of the phytohemagglutinin family of proteins from red kidney beans(Phaseolus vulgaris) by affinity chromatography. - Biochim. Biophys. Acta405:72–81, 1975.
Hall, T. C, Ma, Y., Buchbinder, B. U., Pyne, J. W., Sun, S. M., Bliss, F. A.: MessengerRNA for G1 protein of French bean seeds: cell-free translation and product characterization. -Proc. nat. Acad. Sci. 75:3196–3200, 1978.
Higgins, T. J. V.: Synthesis and regulation of major proteins in seeds. - Annu. Rev. Plant Physiol.35:191–221, 1984.
Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head bacteriophage T4. -Nature227:680–689, 1970.
Leavitt, R. p., Felsted, R. L., Bachur, N. R.: Biological and biochemical properties ofPhaseolus vulgaris isolectins. - J. biol. Chem.252:2961–2980, 1977.
Lioi, L.: Species relationships inPhaseolus: electrophoretic analysis of seed storage proteins. - Biol. Plant.29:49–53, 1987.
Morgan, M. R., Manen, J. F.: Lectin variability inPhaseolus coccineus. - Phytochemistry24:1981–1985, 1985.
NowakovA, N., Kocourek, J.: Isolation and characterization of hemagglutinin from scarlet runner seeds (Phaseolus coccineus L.) - Biochim. Biophys. Acta359:320–332, 1974.
O’Farrel, P. H.: High resolution two-dimensional electrophoresis of proteins.-J. biol. Chem.250:4007–4021, 1975.
Osterman, L. A.: Methods of Protein and Nucleic Acid Research,Vol. 1. - Springer-Verlag, Berlin-Heidelberg-New York-Tokyo 1984.
Racusen, D., Foote, M.: The major glycoprotein in germinating bean seeds. - Can. J. Bot.49:2107–2111, 1971.
Smartt, J.: Interspecific hybridization between cultivated American species of the genusPhaseolus. -Euphytica19:480–489, 1970.
Wright, D. J., Boulter, D.: Purification and subunit structure of legumin ofVicia faba L.- Biochem. J.141:413–425, 1974.
Author information
Authors and Affiliations
Additional information
Part I.
Rights and permissions
About this article
Cite this article
Bernardi, R., Lupi, M.C. & Durante, M. Phaseolus coccineus L. Storage Proteins. Extraction and Characterization. Biol Plant 32, 198–204 (1990). https://doi.org/10.1007/BF02890873
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02890873