Summary
Non-pigmented tumor cells of B16-XI mouse melanoma were found to contain a diploid number of chromosomes similarly to those of melanotic tumors and the parental cells in tissue culture. A major difference between pigmented and non-pigmented cells was in the number of biarmed chromosomes per cell. There was no difference in growth rate between non-pigmented and pigmented tumors, but growth usually begins about 2 days earlier in the former. Pigmentation lost in the course of serial transplantation was restored by irradiating the non-pigmented tumor continuously with 2,500–3,000 rads/passage of X-rays during six transfer generations. In the course of repeated irradiations, the chromosomes changed structurally and numerically as the pigmentation of the tumor was gradually restored. The observations of tumor growth and chromosomal changes are discussed in relation to the pigmentation of B16-XI melanoma cells.
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References
Davidson RL, Ephrussi B, Yamamoto K (1966) Regulation of melanin synthesis in mammalian cells, as studied by somatic hybridization. Proc Natl Acad Sci USA 56:1437–1440
Davidson RL, Yamamoto K (1968) Regulation of melanin synthesis in mammalian cells, as studied by somatic hybridization. II. The level of regulation of 3,4-dihydroxyphenylalanine oxidase. Proc Natl Acad Sci USA 60:894–901
Davidson RL (1972) Regulation of melanin synthesis in mammalian cells: Effects of gene dosage on the expression of differentiation. Proc Natl Acad Sci USA 69:951–955
Demopoulos HB, Kasuga T, Channing AA, Bagdoyan H (1965) Comparison of ultrastructure of B16 and S-91 mouse melanomas, and correlation with growth patterns. Lab Invest 14:108
Evans HJ (1962) Chromosome aberrations induced by ionizing radiations, Int Rev Cytol 13:221–321
Fitzpatrick TB, Seiji SM, Mcgugan AD (1961) Melanin pigmentation. New Eng J Med 266:328–333
Fougere C, Ruiz F, Ephrussi B (1972) Gene dosage dependence of pigment synthesis in melanoma × fibroblast hybrids. Proc Natl Acad Sci USA 69:330–334
Furuse T, Kasuga T (1982) The radiation effects of 2 MeV fast neutrons on B16 mouse melanoma. Nipp Act Radiol (in Japanese with English abstract 42:476–484)
Hasumi K, Sakamoto G, Sugano H, Kasuga T, Masubuchi K (1978) Primary malignant melanoma of vagina. Study of four autopsy cases with ultrastructural findings. Cancer 42:2656–2686
Hu F, Lesney PF (1964) The isolation and cytology of two pigment cell strains from B16 mouse melanomas. Cancer Res 24:1634–1643
Kasuga T, Furuse T, Takahashi I, Kubo E (1971) Ultrastructural and autoradiographic studies on melanin synthesis and membrane system using cultured B16 melanoma. In: Kawamura T, Fitzpatric TB, Seiji M (eds) Biology of normal and abnormal melanocytes. Held in Tokyo, October, 1969, University of Tokyo Press, Tokyo
Levisohn SR, Thompson B (1972) Tyrosine aminotransferase induction regulation variant in tissue culture. Nature 235:102–104
Nakayasu M, Saeki H, Tohda H, Oikawa A (1977) Effects of sugars on melanogenesis in cultured mammalian cells. J Cell Physiol 92:49–56
Nicolaus RA (1968) Melanins, Herman, Paris
Novikoff AB, Albala A, Biempica LJ (1968) Ultrastructural and cytochemical observations on B16 and Harding-Passey mouse melanoma. J Histochem Cytochem 16:299–319
Pawelek J, Sansone M, Morowitz J, Moellmann G, Godawska E (1974) Genetic control of melanization: Isolation and analysis of amelanotic variants from cultured melanotic melanoma cells. Proc Natl Acad Sci USA 71:1073–1077
Satoh GJ, Mishima Y (1967) Tyrosinase inhibitor in Fortner’s amelanotic and melanotic malignant melanoma. J Invest Dermatol 48:301–303
Satoh S, Takeuchi T, Oikawa J (1978) Regulation of melanin synthesis in cultured melanoma cells. Tiss Culture 4:320–332
Silagi S, Bruce SA (1970) Supression of malignancy and differentiation on melanotic melanoma cells. Proc Natl Acad Sci USA 66:72–78
Silagi S (1976) Effects of bromouridine on tumorigenecity, immunogenicity, and virus production, plasminogen activator, and melanogenesis in mouse melanoma cells. Int Rev Cytol 45:65–111
Urano M, Fukuda N, Ando K, Koike S, Tanaka N (1974) Tumor control and regrowth probability after a single radiation of experimental animal tumor. Nat Cancer Inst 53:517–525
Varga JM, Dipasquale A, Pawelek J, Mcquire JS, Lerner AB (1974) Regulation of melanocyte stimulating hormone action at the receptor level. Discontinuous binding of hormone to synchronized mouse melanoma. Proc Natl Acad Sci USA 71:1590–1593
Vogt M (1959) A study of the relationship between karyotype and phenotype in cloned lines of strain HeLa. Genetics 44:1257–1270
Wrathall JR, Oliver C, Silagi S, Essner EJ (1973) Suppression of pigmentation in mouse melanoma cells by 5-bromodeoxyuridine. Effects of tyrosinase activity and melanosome formation. J Cell Biol 57:406–423
Wong G, Pawelek J (1975) Melanocyte-stimulating hormone promotes activation of pre-existing tyrosinase molecules in Cloudman S 91 melanoma cells. Nature 255:644–646
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Ohara, H., Kubo, E., Furuse, T. et al. Induction of pigmentation by continuous X-irradiation of amelanotic tumors of B16-XI mouse melanoma and induced change in chromosomes of amelanotic cells. Virchows Archiv B Cell Pathol 41, 267–276 (1982). https://doi.org/10.1007/BF02890285
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DOI: https://doi.org/10.1007/BF02890285