Summary
The DNA ploidy pattern and amplification of ERBB and ERBB2 genes were examined in paraffinembedded tissue from gastric carcinomas using flow cytometry and a slot-blot hybridization technique. The incidence of aneuploidy in well differentiated adenocarcinomas (56%) was significantly higher (p<0.05) than that in poorly differentiated adenocarcinomas (21%). The DNA ploidy pattern was not remarkably different between the primary tumors and metastatic deposits in lymph nodes. Of the nine specimens having an aneuploid stem cell line in the primary tumor and/or in metastases, three showed ERBB2 gene amplification and one showed ERBB gene amplification. The incidence of epidermal growth factor (EGF) immunoreactivity in tumor cells showed no difference between diploid and aneuploid tumors. These findings indicate that aneuploidy is frequently associated with amplification of ERBB and ERBB2 genes.
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Dobeau L, Chandler LA, Gralow JR, Nichols PW, Jones PA (1986) Southern blot analysis of DNA extracted from formalin-fixed pathology specimens. Cancer Res 46:2964–2969
Goelz SE, Hamilton SR, Vogelstein B (1985) Purification of DNA from formaldehyde fixed and paraffin embedded human tissue. Biochem Biophys Res Commun 130:118–126
Hedley DW, Friedlander ML, Taylor IW, Rugg CA, Musgrove EA (1983) Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. J Histochem Cytochem 31:1333–1335
Hirose K, Nakagawara G, Hattori T (1988) Correlation between DNA ploidy pattern and EGF expression in human gastric cancers. In: Proceedings of the Japanese Cancer Association, 47th Annual Meeting, pp 356
Japanese Research Society for Gastric Cancer (1981) The general rules for the gastric cancer study in surgery and pathology. Jpn J Surg 11:127–139
Kujari H, Joensuu H, Klemi P, Asola R, Nordman E (1988) A flow cytometric analysis of 23 carcinoid tumors. Cancer 61:2517–2520
Remvikos Y, Muleris M, Vielh P, Salmon RJ, Dutrillaux B (1988) DNA content and genetic evolution of human colorectal adenocarcinoma. A study by flow cytometry and cytogenetic analysis. Int J Cancer 42:539–543
Tahara E, Ito H, Nakagami K, Shimamoto F, Yamamoto M, Sumii K (1982) Scirrhous argyrophil cell carcinoma of the stomach with multiple production of polypeptide hormones, amine, CEA, lysozyme and HCG. Cancer 49:1904–1915
Tahara E, Sumiyoshi H, Hata J, Yasui W, Taniyama K, Hayashi T, Nagae S, Sakamoto S (1986) Human epidermal growth factor in gastric carcinoma as a biologic marker of high malignancy. Jpn J Cancer Res (Gann) 77:145–152
Tomita T, Yasue M, Engelhard HH, Mclone DG, Gonzalez-Crussi F, Bauer KD (1988) Flow cytometric DNA analysis of medulloblastoma. Prognostic implication of aneuploidy. Cancer 61:744–749
Tosi P, Leoncini L, Cintorino M, Vindigni C, Minacci C, Nuti S, Pinto E, Stefano AD, Cevenini G (1988) Flow cytometric analysis of DNA ploidy pattern from deparaffinized formalinfixed gastric cancer tissue. Int J Cancer 42:868–871
Tsuda H, Shimosato Y, Upton MP, Yokota J, Terada M, Ohira M, Sugimura T, Hirohashi S (1988) Retrospective study on amplification of N-myc and c-myc genes in pediatric solid tumors and its association with prognosis and tumor differentiation. Lab Invest 59:321–327
Tsujino T, Nakatani H, Shimosato T, Tahara E (1989) Alterations of oncogenes in metastatic tumors of human gastric carcinomas. In: Proceedings of the Japanese Society of Pathology, 78th Annual Meeting, pp 273
Wong AJ, Ruppert JM, Eggleston J, Hamilton SR, Baylin SB, Vogelstein B (1986) Gene amplification of c-myc and N-myc in small cell carcinoma of the lung. Science 233:461–464
Yasui W, Hata J, Yokozaki H, Nakatani H, Ochiai A, Ito H, Tahara E (1988a) Interaction between epidermal growth factor and its receptor in progression of human gastric carcinoma. Int J Cancer 41:211–217
Yasui W, Sumiyoshi H, Hata J, Kameda T, Ochiai A, Ito H, Tahara E (1988b) Expression of epidermal growth factor receptor in human gastric and colonie carcinomas. Cancer Res 48:137–141
Yokota J, Yamamoto T, Toyoshima K, Terada M, Sugimura T, Battifora H, Cline MJ (1986) Amplification of c-erb B-2 oncogene in human adenocarcinomas in vivo. Lancet 5:765–766
Yokota J, Yamamoto T, Miyajima N, Toyoshima K, Nomura N, Sakamoto H, Yoshida T, Terada M, Sugimura T (1988) Genetic alterations of the c-erb B-2 oncogene occur frequently in tubular adenocarcinoma of the stomach and are often accompanied by amplification of the v-erbA homologue. Oncogene 2:283–287
Yokozaki H (1986) Epithelial phenotypic expression of human foetal gastrointestinal mucosa: An immunohistochemical analysis. Hiroshima J Med Sci 35:207–222
Yoshida K, Tsuda T, Matsumura T, Tsujino T, Hattori T, Ito H, Tahara E (1989) Amplification of epidermal growth factor receptor (EGFR) gene and oncogenes in human gastric carcinomas. Virchows Arch [B] 57:285–290
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Oda, N., Tsujino, T., Tsuda, T. et al. DNA ploidy pattern and amplification of ERBB and ERBB2 genes in human gastric carcinomas. Virchows Archiv B Cell Pathol 58, 273–277 (1989). https://doi.org/10.1007/BF02890081
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DOI: https://doi.org/10.1007/BF02890081