Summary
The morphologic changes of the small intestine after the mechanical obstruction were studied by light and electron microscopy. After the ligation of the upper small intestine, segments of jejunum, both proximal and distal to the site of obstruction, were removed at intervals varying from 45 min to 24 h.
The essential changes were found in the epithelial cells of the tips of villi, and few morphologic differences were recognized between samples proximal and distal to the site of obstruction. The most remarkable changes in the mucosa were the pseudopodlike extension of the cytoplasm, vacuolar alteration of the villus epithelial cells, desquamation of these degenerated cells, and the dilatation of the epithelial intercellular spaces. A few epithelial cells showed hypertrophy of the smooth endoplasmic reticulum. In the sub-mucosa, vascular stasis and edema were observed throughout the course. The mechanism of fluid loss into the intestinal lumen was discussed briefly.
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References
Barnett, W.: Experimental strangulated intestinal obstruction-A review. Gastroenterology39, 34–40 (1960)
Chen, H., Reyes, V., Fresh, J.W.: An electron microscopic study of the small intestine in human cholera. Virchows Arch. Abt. B Zellpath.7, 236–259 (1971)
David, H., Uerlings, I.: Elektronenmikroskopische Befunde am Dünndarm des Kaninches nach Gefäßunterbindung. Exp. Pathol.1, 30–44 (1967)
Ecknauer, R., Clarke, R.M., Meyer, H.: Acute distal intestinal obstruction in gnotobiotic rats. Intestinal morphology and cell renewal. Virchows Arch. B Cell Path.25, 151–160 (1977)
Feldberg, W., Smith, A.N.: Release of histamine by tryptamine and 5-hydroxytryptamine. Br.J. Pharmacol.8, 406–411 (1953)
Field, M.: Progress in gastroenterology. Intestinal secretion. Gastroenterology66, 1063–1084 (1974)
Grace, H.: The handling of water and electrolytes by the small bowel following the relief of intestinal obstruction. Br.J. Surg.58, 760–764 (1971)
Hendrix, T.R., Bayless, T.M.: Digestion: intestinal secretion. Annu. Rev. Physiol.32, 139–164 (1970)
Kaltiala, E.H.: Intestinal 5-hydroxytryptamine in experimental mechanical ileus. Acta Chir. Scand.137, 563–568 (1971)
Kamei, H., Watanabe, T., Takemitsu, T., Monoi, T., Sugimoto, K., Kondo, T.: Cell renewal in experimentally obstructed small intestine in rat. Jpn. J. Surg.3, 237–247 (1973)
Kern, E.: Pathophysiologie des mechanischen Darmverschlusses. Wien. Klin. Wochenschr.84, 437–442 (1972)
Merkle, P., Bindewald, H., Breitig, D.: Untersuchungen zu Funktion und Stoffwechsel der Mucosa beim mechanischen Dünndarmileus der Ratte. Langenbecks Arch. Chir.338, 291–297 (1975)
Mirkovitch, V., Cobo, F., Robinson, J.W.L., Menge, H., Gomba, Sz.: Morphology and function of the dog ileum after mechanical occlusion. Clin. Sci. Mol. Med.50, 123–130 (1976)
Norris, H.T., Majno, G.: On the role of the ileal epithelium in the pathogenesis of experimental cholera. Am. J. Pathol.53, 263–279 (1968)
Otto, H.F.: Über Beobachtungen zum kompletten Paneth-Zell-Schwund bei idiopathischer Steatorrhoe. Beitr. Pathol.143, 378–389 (1971)
Patnaik, B.K., Ghosh, H.K.: Histopathological studies on experimental cholera. Br.J. Exp. Path.47, 210–214 (1966)
Richter, H., Kusche, J.: Neue Aspekte zur Pathophysiologie des Ileus. In: Ileus, Symposion Marburg, November 1976, H. Richter and P. Eckert (eds.), pp. 74–79. Stuttgart: Georg Thieme 1978
Shields, R.: The absorption and secretion of fluid and electrolytes by the obstructed bowel. Br.J. Surg.52, 774–779 (1965)
Speece, A.J.: Histochemical distribution of lysozyme activity in organs of normal mice and radiation chimeras. J. Histochem. Cytochem.12, 384–391 (1964)
Tonner, P.G.: Cytology of intestinal epithelial cells. Int. Rev. Cytol.24, 233–343 (1968)
Turjman, N., Yardley, J.H., Hendrix, T.R., Gotterer, G.S.: Effect of intestinal distention on levels of cyclic AMP and fluid secretion. Johns Hopkins Med. J.140, 97–99 (1977)
Varkonyi, T., Wittmann, T., Varro, V.: Effect of local circulatory arrest on the structure of the enterocytes of the isolated intestinal loop. Digestion15, 295–302 (1977)
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Recipient of an Alexander von Humboldt-Stiftung postdoctoral research fellowship
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Yamamoto, M., Plessow, B., Koch, H.K. et al. Electron microscopic studies on the small intestine of rats after mechanical intestinal obstruction. Virchows Archiv B Cell Pathol 31, 157–168 (1979). https://doi.org/10.1007/BF02889933
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DOI: https://doi.org/10.1007/BF02889933