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Die Feinstruktur des Nitrosomorpholin-induzierten Cholangiofibroms der Ratte

Fine structure of cholangiofibromas induced in the rat by N-nitrosomorpholine

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Virchows Archiv B

Summary

11 cholangiofibromas induced in the rat by nitrosomorpholine were investigated by electron microscopy and cytochemistry. The epithelial component of cholangiofibromas is characterized by mucus-producing tubules which are surrounded by basal lamina and show an apical brush border with marked glycocalyx. The basal laminae may be split into several layers. Neighbouring duct epithelial cells show marked interdigitations. Desmosomes often appear in large numbers. The cytoplasm of the epithelial tumor cells is rich in ribosomes and/or granular ER. In many cells large bundles of tonofilaments are developed. Golgi complexes are numerous and usually large. The tumor tubules often contain goblet cells which produce acid and neutral mucopolysaccharides. In addition, glycogen storage cells are sometimes included in the tubules. Like the other duct epithelial cells they have a basal lamina and an apical brush border (or microvilli). The glycogen is mainly stored in the form of large aggregates of single particles (β-particles) in the cytoplasmic matrix. Sometimes large glycogen-containing autophagic vacuoles are found. Lipoid bodies with lamellar structure can be detected sporadically. Microbodies (peroxisomes) are completely absent. The mesenchymal component of the tumors consists mainly of fibroblasts, collagen fibres and capillaries. Fibroblasts and endothelial cells often include large bundles of cytoplasmic microfilaments. Mast cells are regularly and loosely embedded in the tumor tissue. All these findings suggest that the cholangiofibromas are derived from carcinogen-induced cholangiofibroses and that the epithelial tumor component stems from intrahepatic bile duct epithelia, the polysaccharide metabolism of which is disturbed during the neoplastic transformation.

Zusammenfassung

11 Nitrosomorpholin-induzierte Cholangiofibrome der Ratte wurden elektronenmikroskopisch und cytochemisch untersucht. Die epitheliale Komponente der Tumoren ist durch schleimbildende tubuläre Formationen gekennzeichnet, die in der Regel von einer Basalmembran umgeben sind und apical einen Bürstensaum mit ausgeprägter Glycocalyx aufweisen. Die Basalmembran kann in mehrere Lagen aufgesplittert sein. Benachbarte Gangepithelien sind stark miteinander verzahnt, Desmosomen liegen oft in groβer Zahl vor. Das Cytoplasma der Tumorepithelien ist reich an Ribosomen und/oder granulärem ER. In vielen Zellen sind umfangreiche Bündel von Tonofilamenten entwickelt. Golgikomplexe sind meist ausgedehnt und zahlreich. Häufig enthalten die Tumorgänge Becherzellen, die sowohl saure als auch neutrale Mucopolysaccharide produzieren. Daneben sind mitunter Glykogenspeicherzellen in die Gänge eingefügt. Sie sind ebenso wie die übrigen Gangepithelien mit einer Basalmembran und einem apicalen Bürstensaum (oder Mikrovilli) versehen. Das Glykogen wird in monopartikulärer Form überwiegend im Grundplasma, gelegentlich auch in groβen autophagen Vacuolen gespeichert. Vereinzelt finden sich lamellar geschichtete Lipoidkörper. Mikrokörper (Peroxisomen) fehlen. Die mesenchymale Komponente der Tumoren besteht hauptsächlich aus Fibroblasten, Kollagenfibrillenund Capillaren. Fibroblasten wie Capillarendothelien enthalten oft Bündel von cytoplasmatischen Mikrofilamenten. Locker eingestreut in das Tumorbindegewebe finden sich regelmäβig Mastzellen. Alle Befunde sprechen für eine Herkunft der Cholangiofibrome von Carcinogen-induzierten Cholangiofibrosen und für eine Abstammung der epithelialen Tumorkomponente von intrahepatischen Gallengangsepithelien, die während der neoplastischen Transformation Regulationsstörungen im Polysaccharidstoffwechsel erfahren.

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  1. Abteilung für Cytopathologie, Institut für Experimentelle Pathologie am Deutschen Krebsforschungszentrum Heidelberg, Heidelberg 1, D-6900, Bundesrepublik, Deutschland

    P. Bannasch & B. Massner

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  1. P. Bannasch
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  2. B. Massner
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Bannasch, P., Massner, B. Die Feinstruktur des Nitrosomorpholin-induzierten Cholangiofibroms der Ratte. Virchows Arch. B Cell Path. 24, 295–315 (1977). https://doi.org/10.1007/BF02889287

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