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Y specific sequence gene analysis of single fetal nucleated erythroblasts from the peripheral blood of pregnant women

Journal of Tongji Medical University volume 21, pages 163–165 (2001)Cite this article

Summary

The single cell isolation technique was used to detect fetal nucleated erythroblasts (FNR-BCs) at a single cell level from the peripheral blood of pregnant women in order to investigate the feasibility of this method for noninvasive prenatal diagnosis. Single fetal nucleated erythroblasts were isolated from the peripheral blood samples from 51 pregnant women (14 to 26 weeks of gestation) by micromanipulation techniques after density gradient centrifugation. Nested polymerase chain reaction method was used to amplify the SRY gene. It was found that the concordance rate of amplification results with real fetal sex was 82.61 %. The sensitivity and specificity were 80 % and 87.50 % respectively. It was suggested that it is feasible and promising in non invasive prenatal diagnosis to detect fetal nucleated erythroblasts at a single cell level by using micromanipulation techniques.

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Affiliations

  1. Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan

    Wang Taoran, Chen Hanping, Lu Yunping & Ma Tingyuan

Authors
  1. Wang Taoran
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  2. Chen Hanping
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  3. Lu Yunping
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  4. Ma Tingyuan
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Additional information

This project was supported by a grant from Science Foundation of Ministry of Public Heath of China (No. 96. 2-112) and a grant from Hubei Provincial National Natural Science Foundation (96J068).

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Taoran, W., Hanping, C., Yunping, L. et al. Y specific sequence gene analysis of single fetal nucleated erythroblasts from the peripheral blood of pregnant women. Journal of Tongji Medical University 21, 163–165 (2001). https://doi.org/10.1007/BF02888086

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  • DOI: https://doi.org/10.1007/BF02888086

Key words

  • single cell
  • peripheral blood of pregnant woman
  • fetal nucleated erythroblasts
  • nested polymerase chain reaction