Summary
131I-labelled anti-thymoglobuline (ATG),131I-labelled immunoconjugate ATG-Dex-DNR and131 I-labelled Ts-MoAb as control antibody, respectively, were injected by intraperitoneal (i. p.) administration into nude mice used as models for human T-cell leukemia. SPECT imaging was performed from day 1 to day 8 following i. p. injection. The results showed that radioimmunoimaging of human tumor xenografts was clearest day 3 after injection in both of ATG and ATG-Dex-DNR groups, whereas it’s not the case in Ts-MoAb group. Nude mice were killed 8th day after injection with antibody or conjugate. The tumor, as well as different dissected normal organs including heart, liver, lungs, kidney, femur and intestine, were harvested, weighed precisely, and radioiodine-counted. T/NT ratios in experimental group was greater than 1.0 (ranged from 1.246 –7.865), and in control group they were less than 1.0 (ranged from 0.263 – 0.757, except for tumor/femur ratio). Our results indicated that ATG and ATG-Dex-DNR had specific affinity to cell line of Tcell leukemia CEM.
Similar content being viewed by others
References
McAb 1992; 12 (4): 223
Macdonald F, Crowson M C, Allum W H et al. In Vivo studies on the uptake of radiolabelled antibodies by colorectal and gastric carcinoma xenografts. Cancer Immunol Immunother, 1986: 23 (2): 119
1992; 221 (4): 230
Hurwitz Esther, Schechter Bilha, Arnon Ruth et al. Binding of anti-tumor immunoglobulins and their daunomycin conjugates to the tumor and its metastases, in Vitro and in Vivo studies with lewis lung carcinoma. Int J Cancer, 1979; 24: 461
1994; 23 (6): 482
Noguchi-A, Takahashi-T, Yamaguchi-T et al. Tumor localization and in vivo anti-tumor activity of the immunoconjugate composed of anti-human colon cancer monoclonal antibody and mitomycin C dextran conjugate. Jpn J Cancer Res, 1991; 82 (2): 219
111In 1992; 12 (1): 18
Buchsbaum Donald J, Wahl Richard L, Glenn Stephan D et al. Improved deliver of radiolabeled anti-B1 MoAb to Raji lymphoma xenografts by predosing with unlabeled anti-Bl Monoclonal Antibody. Cancer Res, 1992, 52 (3): 637
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dong-hua, Z., Jin-zhi, T., Zhi-xiong, L. et al. Localization and biodistribution of conjugate ATG-Dex-DNR in nude mice as models for human leukemia. Journal of Tongji Medical University 15, 82–86 (1995). https://doi.org/10.1007/BF02887907
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02887907