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Expression of angiopoietin-2 gene and its receptor Tie2 in hepatocellular carcinoma

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Summary

To explore the relationship of angiogenesis-related angiopoietin-2 gene and its receptor Tie2 with angiogenesis and the biology, of hepatocellular carcinoma (HOC), angiopoietin-2 gene, Tie2 and CD34 protein expression in 22 resected HOC, 8 cirrhotic and 8 control liver specimens were investigated byin situ hybridization and immunohistochemistry respectively, and the level of angiopoietin-2 and Tie2 expression in HCC were compared in terms of tumor biological parameters. It was found that CD34 was not expressed in control liver, expressed scarcely in cirrhotic liver (17. 8±13.5/HP), but intensively expressed in HCC (86. 3±34. 8/HP,P<0.01). Tie2 receptor was not expressed in controls, expressed at low level in cirrhotic liver (11. 3±8.7/HP), while strongly positive in the microvascular endothelia of HCC (52. 4±16. 7/HP,P<0.01). The level of Tie2 receptor expression in HCC was closely related with tumor diameter, angiogenesis and portal invasion. Angiopoietin-2 gene was not expressed in control liver, expressed mildly in cirrhotic liver (11.2±9. 7/HP), but extensively in tumor zone (36. 4±17. 5/HP), the level of angiopoietin-2 expression was closely related with angiogenesis, portal invasion and histological grading of HCC. It is concluded that angiogenesis is increased in HCC; angiopoietin-2/Tie2 expression in human hepatic carcinoma is closely related with angiogenesis, which are probably involved in the HCC angiogenesis regulation, promoting the development and metastasis of human hepatic cancer.

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References

  1. Folkman J. Tumor angiogenesis: therapeutic implication. New England J Med, 1971,285:1182

    Article  CAS  Google Scholar 

  2. Torimura T, Sata M, Ueno Tet al. Increased expression of vascular endothelial growth factor is associated with tumor progression in hepatocellular carcinoma. Human Pathol, 1998,29:986

    Article  CAS  Google Scholar 

  3. Santo T, Yozawa Y, Deutsch Uet al. Distinct roles of the tyrosine kinase receptors Tie-1 and Tie-2 in blood vessel formation. Nature, 1995,376:70

    Article  ADS  Google Scholar 

  4. Peters K G, Coogan A, Berry Det al. Expression of Tie2/Tek in breast tumor vasculature provides a new marker for evaluation of tumor angiogenesis. Br J Cancer, 1998,77:51

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Siemeister G, Schirner M, Weindel Ket al. Two independent mechanisms essential for tumor angiogenesis: Inhibition of human melonoma xenograft growth by interfering with either the vascular endothelial growth fac(Continuted from page 230) tor receptor pathway or the Tie2 pathway. Cancer Res, 1999,59:3185

    CAS  PubMed  Google Scholar 

  6. Davis S, Aldrich T H, Jones P Fet al. Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning. Cell, 1996,87:1161

    Article  CAS  PubMed  Google Scholar 

  7. Maisonpierre P C, Suri C, Jones P Fet al. Angiopoietin-2, a natiral antagonist for Tie2 that disruptsin vivo angiogenesis. Science, 1997,277:55

    Article  CAS  PubMed  Google Scholar 

  8. Stratmann A, Risau W, Plate K H. Cell type-specific expression of angiopoietin-1 and angiopoietin-2 suggests a role in glioblastoma angiogenesis. Am J Pathol, 1998, 153:1459

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Tanaka S, Mori M, Sakamoto Yet al. Biologic significance of angiopoietin-2 expression in human hepatocellular carcinoma. J Clin Invest, 1999,103:341

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Lin P, Buxton J A, Acheson Aet al. Antiangiogeneic gene therapy targeting the endothelium-specific receptor tyrosine kinase Tie2. Proc Natl Acad Sci USA, 1998, 95:8829

    Article  ADS  CAS  PubMed  PubMed Central  Google Scholar 

  11. Takahama M, Tsutsumi M, Tsujiuchi Tet al. Enhanced expression of Tie2, its ligand Angiopoietin-1, vascular endothelial growth factor, and CD31 in human non-small cell lung cancer. Clin Cancer Res, 1999, 5:2506

    CAS  PubMed  Google Scholar 

  12. Holash J, Maisonpierre P C, Compton Det al. Vessel Cooption, Regression, and Growth in Tumors Mediated by Angiopoietins and VEGF. Science, 1999,284:1994

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of General Surgery, Xiehe Hospital, Tongji Medical School, Huazhong University of Science and Technology, 430022, Wuhan

    Chen Libo (Lecturer), Yang Zhen, Wang Guobin & Wang Chunyou

  2. Department of General Surgery, Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology, 430030, Wuhan

    Yang Zhen

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  1. Chen Libo
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  2. Yang Zhen
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  3. Wang Guobin
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  4. Wang Chunyou
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Libo, C., Zhen, Y., Guobin, W. et al. Expression of angiopoietin-2 gene and its receptor Tie2 in hepatocellular carcinoma. Current Medical Science 21, 228–230 (2001). https://doi.org/10.1007/BF02886437

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  • DOI: https://doi.org/10.1007/BF02886437

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