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Application of CD45/SSC gating multiparameter flow cytometry in the classification of acute leukemia — An analysis of 139 cases

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Summary

In order to study the significance of flow cytometry immunophenotyping in the diagnosis of acute leukemia, CD45/SSC gating multiparameter flow cytometry (FCM) was utilized to analyze the immunophenotypes of 139 cases of acute leukemia. 139 cases of acute leukemia were enrolled in our hospital from April 1998 to April 2000. Morphological analysis and FCM immunophenotypic tests were conducted on all cases. Our results showed that CD45/SSC gating multiparameter flow cytometry immunophenotyping could reflect the origin of leukemic cells specifically. It is one of the important methods for the diagnosis of ALL, AML, and HAL. CD45/SSC gating multiparameter FCM analysis is a good technique for immunophenotyping. FCM immunophenotypic analysis can help improve the diagnosis and classification of acute leukemia, and extend the use of FCM in clinical practice.

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Authors and Affiliations

  1. Institute of Hematology, Xiehe Hospital, Tongji Medical College, Huazhong Science and Technology University, 430022, Wuhan

    Li Weiming (Resident), Chen Zhichao, Liu Zongping & Zou Ping

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  1. Li Weiming
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  2. Chen Zhichao
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  3. Liu Zongping
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  4. Zou Ping
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Weiming, L., Zhichao, C., Zongping, L. et al. Application of CD45/SSC gating multiparameter flow cytometry in the classification of acute leukemia — An analysis of 139 cases. Current Medical Science 21, 209–211 (2001). https://doi.org/10.1007/BF02886431

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  • DOI: https://doi.org/10.1007/BF02886431

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