Abstract
Antral follicles are present in the ovaries throughout gestation. These follicles are “physically immature” and cannot ovulate under the induction of LH/hCG. The purpose of the present study is to examine whether follicles in pregnant rat ovaries are capable of steroidogenesis. StAR is believed to be the key regulator of steroid hormone biosynthesis. Antisense StAR probe and anti-StAR rabbit serum have been used to detect the StAR expression in ovarian follicles at various stages of pregnant rats. The results indicate that theca-interstitial cells and the membrane granulosa cells at the stages from the estrous or pre-estrous in the normal cycling rat ovary express StAR mRNA and its protein, whereas neither granulosa cells nor the theca cells in pregnant ovary throughout gestation express StAR. These results indicate that the pregnant follicles throughout gestation are incapable of steroidogenesis.
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References
Slocco, D.M., A StAR search: implications in controlling steroidogenesis,Biol. Reprod., 1997, 56: 328.
Clark, B.J., Wells, J., King, S.R.et al., The purification, cloning, and expression of a novel luteinizing hormone-induced mitochondrial protein in MA-10 mouse Leydig tumor cells,J. Biol. Chem., 1994, 269: 28314.
Clark, B. J., Soo, S. C., Caron, K.M.et al., Hormonal and developmental regulation of the steroidogenic acute regulatory protein,Mol. Endocrinol., 1995, 9: 1346.
Choi, Y. S., Stocco, D.M., Freeman, D.M., Diethylumbelliferyl phosphate inhibits steroidogenesis by interfering with a long-lived factor acting between protein kinase A activation and induction of the steroidogenic acute regulatory (StAR) protein,Eur. J. Biochem., 1995, 234: 680.
Lin, D., Sugawara, T., Strauss III. J. F.et al., Role of steroidogenic acute regulatory protein in adrenal and gonadol steroidogenesis,Science, 1995, 267: 1828.
Caron, K.M., Soo, S. C., Wetsel, W. B.et al., Targeted disruption of the mouse gene encoding steroidogenic acute regulatory protein provides insights into congenital lipoid adrenal hyperplasia,Proc. Natl. Acad. Sci. USA, 1997, 92: 11540.
Saito, T., Saxena, B.B., Specific receptors for prolactin in the ovary,Acta Endocrinol. Copenh., 1975, 80: 126.
Tsai-Morris, C.H., Ghosh, M., Hirshfield, A.N., Inhibition of ovarian aromatase by prolactinin vitro, Biot. Reprod., 1983, 29: 342.
Hu, Z.Y., Liu, Y.X., Effect of prolactin on gonadotropin-induced ovarian estrogen and progesterone production in mouse,Acta Physiologica Sinica, 1995, 47: 96.
McNatty, K.P., Sawers, R.S., McNeilly, A.S., A possible role of prolactin in control of steroid secretion by the human Graafian follicle,Nature, 1974, 250: 653.
McNeilly, A.S., Prolactin and the control of Gonadotropin secretion,J. Endocrinol., 1987, 115: 1.
Liu, Y.X., Peng, X.R., Chen, Y.J.et al., Prolactin delays hCG-induced ovulation and down-regulates plasminogen activator system in rat ovary,Human Reprod., 1997, 12: 2748.
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Chen, Y., Gao, H. & Liu, Y. Follicles in pregnant rat ovary are incapable of steroidogenesis. Chin.Sci.Bull. 44, 1797–1801 (1999). https://doi.org/10.1007/BF02886162
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DOI: https://doi.org/10.1007/BF02886162