Abstract
Gene mapping of a mouse coat mutation has been investigated. First, 100 10-bp random primers were used to amplify DNA, but the mutation could not be located by this method because there were no correlation between the amplified products and coat phenotypes. Second, by usingIdh1, Car2, Mup1, Pgb1, Hbb, Es10, Es1, Mod1, Gdc1, Ce2, Es3 as genetic markers, linkage test crosses (two-point test) consisting of intercrossing uncovered BALB/c mice (homozygotes) to CBA/N and C57BV6 mice with normal hair and backcrossing the heterozygotes of the F1 to the uncovered BALB/c mice were made. It was soon evident that the mutation was linked toEs3 on chromosome 11. Furthermore, three-point test was made by usingEs3 and D11Mit8 (a microsatellite DNA) as genetic markers. The result showed that the mutation was linked toEs3 with the percentage recombination of (7.89 ± 2.19)%, and linked to Dl1Mit8 with the percentage recombination of (26.30± 3.57)%. The percentage recombination betweenEs3 and D11Mit8 was (32.90±3.81)%. The mutation was named Uncovered, with the symbolUncv. According to the recombinations, the loci order was D11Mit8-26.30±3.57-Uncv- 7.89 -2.19-Es3. From the location on the chromosome, it was concluded that the mutation was a new mutation which affected the skin and hair structure of mouse. TheUncv has entered MGD (Mouse Genome Database).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Hogan, B., Frank, C., Elisabeth, L.,Manipulating the Mouse Embryo-A Laboratory Manual, New York: Cold Spring Harbor Laboratory, 1986, 1.
Dietrich, W. H., Katz, S. E., Lincoln, H. S.et al., A genetic map of the mouse suitable for typing inhaspecific crosses,Genetics, 1992, 131: 423.
Dietrich, F. W., Miller, T., Steen, R. A., Comprehensive genetic map of the mouse genome,Nature, 1996, 380: 149.
Li, S. R., Wang, D. P., Shi, Y. Y.et al., Genetic characteristics of a new coat mutant mouse,Chinese J. Veterinry Sciences (in Chinese), 1995, 19(1): 26.
Yu, X. L., Li, S. R., Wu, N., A histological study on the skin and hair of the new mutant mouse,Chinese J. Veterinay Sciences (in Chineae), 1996. 16(3): 299.
Ausue, M. A., Brent, R., Kingston, E. R.et al., Short Protocols in Molecular Biology, New York: John Wiley and Sons Inc., 1995.
Zhang, W. Y., Gong, C. M., Wei, Y. L.et al., A new way for inbred strain mice genetic monitoring and the discovery of sex linking RAPD markers,Acta Biologiae Experimentalis Sciencia (in Chinese), 1996. 29(1): 59.
Jiang, P., Zhang, Y. L., Zhou, D. Y.,Laboratory Manual on Molecular Biology, Beijing: People’s Military Medical Publishing House, 1996.
Williams, J. G., Kubelik, K. A. R., Livak, K.J., DNA polymorphisms amplified by arbitrary primers are useful as genetic markers,Nucleic. Acids. Res., 1990, 18: 6531.
Welsh, J., McClelland, M., Fingerprinting genomes using PCR with arbitmy primers,Nucleic. Acids. Res., 1990, 18 (24): 7213.
Foster, H. L., Small, J. D., Fox, J. D.,Thc Mouse in Biomedical Reaserch, Vol. 1, New York: Academic Press, 1981.
Peters, J., Selley, R. L., Cocking, Y., Mouse gene list,MOUSE Genome, 1997, 94(2): 1.
Witherden, S. A., Nicholson, J. S., Fisher, E. M. C., The value of mouse mutants, MouseGenome, 1997, 95(4): 856.
Green, E. L.,Genetics and Probability in Animal Breeding Experiments, London: Macmillan Publishers Ltd., 1981.
Author information
Authors and Affiliations
About this article
Cite this article
Li, S., Wang, D., Lan, H. et al. Gene mapping of a new coat mutation in mouse. Chin.Sci.Bull. 44, 1207–1210 (1999). https://doi.org/10.1007/BF02885967
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02885967