Skip to main content
SpringerLink
Log in

The use of a resin adsorbent to isolate cytokinins from sea water

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

XAD-4 resin was used to isolate cytokinins present in Baltic sea water. Four compounds were tentatively identified as 6-(3-methylbut-2-enylamino) purine and zeatin, and their ribosides.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

IPA:

6-(3-methylbut-2-enylamino)purine

IPAR:

6-(3-methylbut-2-enylanino)-9-β-D-ribofuranosylpurine

Z:

zeatin, 6-(4-hydroxy-3-methylbut-2-enylamino) purine

ZR:

zeatin riboside, 6-(4-hydroxy-3-methylbut-2-enylamino)-9-β-D-ribofuranosylpurine

BSTFA:

N, O-bis-(trimethylsilyl)-trifluoroacetamide

References

  • Andersson, B., Andersson, K.: Use of Amberlite XAD-7 as a concentrator column in the analysis of endogenous plant hormones. - J. Chromatogr.242: 353–358. 1982.

    Article  CAS  Google Scholar 

  • Armstrong, D. J., Burrows, W. J., Evans, P. K., Skoog, F.: Isolation of cytokinins from t-RNA. - Biochem. biophys. Res. Commun.37: 451–456, 1969.

    Article  PubMed  CAS  Google Scholar 

  • Augier, H.: Les hormones des algues. Etat actuel des connaissances. II - Recherche et tentatives d’identification des gibbérellines, dos cytokinines et de diverses autres substances de nature hormonale. - Bot. Mar.19: 245–254, 1976.

    Article  CAS  Google Scholar 

  • Biddington, N. L., Thomas, T. H.: A modifiedAmaranthus betacyanin bioassay for the rapid determination of cytokinins in plant extracts. - Planta111: 183–186, 1973.

    Article  CAS  Google Scholar 

  • Dauphin, B., Teller, G., Durand, B.: Mise au point d’une nouvelle méthode de purification, caractérisation et dosage de cytokinines endogènes, extraites de bourgeons de Mercuriales annuelles. - Physiol. vég.15: 747–762, 1977.

    CAS  Google Scholar 

  • Kemp, T. R., Andersen, R. A., Oh, J., Vaughn, T. H.: High-resolution gas ehromatography of methylated ribonucleosides and hypermodified adenosines. - J. Chromatogr.241: 325 to 332, 1982.

    Article  Google Scholar 

  • Kentzer, T., Synak, R., Burkiewicz, K., Banaś, A.: Cytokinin-like activity in sea water andFucus vesiculosus L. - Biol. Plant.22: 218–225, 1980.

    CAS  Google Scholar 

  • Kim, S.-G., Chatfield, J. M., Armstrong, D. J.: Trace enrichment of cytokinms using neutral polystyrene resins. - J. Chromatogr.321: 175–183, 1985.

    Article  CAS  Google Scholar 

  • Pedersén, M.: Identification of a cytokinin 6-(3-methyl-2-butenylamino) purine in sea water and the effect of cytokinins on brown algae, - Physiol. Plant.28: 101–105, 1973.

    Article  Google Scholar 

  • Pedersén, M., Fridborg, G.: Cytokinin-like activity in sea water from theFucus —Ascophyllum zone. - Experientia28: 111–112, 1972.

    Article  PubMed  Google Scholar 

  • Stafford, A. E., Kunhle, J. A., Corse, J., Hautala, E.: Isolation and purification of plant hormones by XAD-2 column ehromatography. - J. Chromatogr.294: 485–488, 1984.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Physiology, University of Gdańsk, 81-378, Gdynia, Poland

    R. Synak, Iwona Zarebska & Teresa Kentzer

Authors
  1. R. Synak
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Iwona Zarebska
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Teresa Kentzer
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Synak, R., Zarebska, I. & Kentzer, T. The use of a resin adsorbent to isolate cytokinins from sea water. Biol Plant 28, 412–416 (1986). https://doi.org/10.1007/BF02885043

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02885043

Keywords

Search

Navigation