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Human neuronal tau promoting the melting temperature of DNA

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Chinese Science Bulletin

Abstract

The hyperchromic effect of ultraviolet spectroscopy shows that adding recombinant human neuronal tau to the solution of calf thymus DNA will promote the melting temperature (T m) from 67°C to 81°C. Similar result has been detected when adding tau to plasmid p Bluescript-II SK, by raising Tm from 75°C to 85°C. The kinetics of thermal denaturation of DNA with tau is much slower than that of control. It suggests that tau may stabilize the double helix conformation of DNA.

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Authors and Affiliations

  1. Laboratory of Visual Information Processing, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China

    Qian Hua & Rongqiao He

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  1. Qian Hua
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  2. Rongqiao He
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Correspondence to Rongqiao He.

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Hua, Q., He, R. Human neuronal tau promoting the melting temperature of DNA. Chin. Sci. Bull. 45, 999–1002 (2000). https://doi.org/10.1007/BF02884979

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  • DOI: https://doi.org/10.1007/BF02884979

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