Abstract
In order to identify the genes associated with glioblastoma differentiation, some ESTs, expressed differentially in the control cell and the differentiated human glioblastoma cell line BT-325 induced by the all-trans retinoid acid, have been isolated by the method of DDRT-PCR. Of the 46 ESTs sequenced, 19 are from new genes. A full-length 1 535-bp cDNA, termed geneGDR1, has been isolated from the human cDNA library using the probe designed according to one of the novel ESTs, HGBB098. The open reading frame ofGDR1 gene encodes a putative protein containing 334 amino acid residues. Blast against the current GenBank DNA and protein sequence database did not reveal significant homology with any known proteins. RT-PCR shows thatGDR1 mRNA level increased in the differentiated BT-325 cells after being treated with RA. The different expression patterns ofGDR1 mRNA in human tissues have been detected through the multiple tissue Northern blot hybridization.
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References
Funari, F. B., Huang, H. J., Cavenee, W. K., Genetics and malignant progression of human brain tumors, Cancer Surv., 1995, 25: 233.
Wong, A. J., Bigner, S. H., Bigner, D. D. et al., Increasted expression of the epidermal growth factor receptor gene in malignant gliomas is invariably associated with gene amplification, Proc. Natl. Acad. Sci. USA, 1987, 84: 6899.
Muleris, M., Almeida, A., Dutrillaux, A. M. et al., Oncogene amplification in human gliomas: a molecular cytogenetic analysis, Oncogene, 1994, 9: 2717.
Sehgal, A., Molecular changes during the genesis of human gliomas, Seminar Surg. Oncol., 1998, 14: 3.
Zhao, N., Hashuda, H., Takahashi, N. et al., High density cDNA filter analysis: a novel approach for large-scale, quantitative analysis of gene expression, Gene, 1995, 156: 207.
Shalon, D., Smith, S. J., Brown, P. O., A DNA microarray system for analyzing complex DNA samples using two-color fluorecent probes, Genome Res., 1996, 6: 639.
Hendrick, S. M., Cohen, D. I., Nielsen, E. A. et al., Isolation of cDNA clones encoding T cell-specific membrane-associated proteins, Nature, 1984, 308: 149.
Hubank, M., Schatz, D. G., Identifying differences in mRNA expression by representational difference analysis of cDNA, Nucleic Acid Res., 1994, 22(25): 5640.
Velculescu, V. E., Zhang, L., Vogelstein, B. et al., Serial analysis of gene expression, Science, 1995, 270(5235): 484.
Liang, P., Pardee, A., B., Differential display of eucaryotic messenger RNA by means of the polymerase chain reaction, Science, 1992, 257: 967.
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Li, G., Jin, H., Hu, S. et al. Cloning and identification of a novel human glioma differentiation related geneGDR1 . Chin. Sci. Bull. 45, 995–999 (2000). https://doi.org/10.1007/BF02884978
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DOI: https://doi.org/10.1007/BF02884978