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Isolation and characterization of glycocalyx inStreptomyces aureofaciens

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Abstract

The surface layer, considered to be glycocalyx according to electron-microscopic observations, was separated from a lowproduction strain ofStreptomyces aureofaciens by solubilization with urea and subsequent sonication. The isolation procedure was developed using various agents; neutral phosphatase served as a marker indicating the amount of the material released. The peripheral structure consisted predominantly of glycoprotein and differed from S-layers.

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Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Microorganisms, Pushchino, Moscow Region, USSR

    J. Shabalin & I. Kulayev

  2. Institute of Microbiology, Czechoslovak Academy of Sciences, Vídeňská 1083, 142 20, Prague 4

    I. Beneš & Z. Hošťálek

Authors
  1. J. Shabalin
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  2. I. Beneš
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  3. Z. Hošťálek
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  4. I. Kulayev
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Shabalin, J., Beneš, I., Hošťálek, Z. et al. Isolation and characterization of glycocalyx inStreptomyces aureofaciens . Folia Microbiol 36, 465–467 (1991). https://doi.org/10.1007/BF02884067

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  • DOI: https://doi.org/10.1007/BF02884067

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