Abstract
The production of bacterial enzymes in batch fermentations is compared with results obtained in continuous culture. When studying the production of α-amylase inBacillus subtilis it was found that instability of the enzyme synthesis was due to nonhomogeneity of the population rather than to “the culture’s history” (i.e. succession of several physiological states necessary for the enzyme production). The plasmid contained in the production clone was found to be the factor responsible for the α-amylase production. Predominance of the production clone or of the nonproduction one depends on the cultivation conditions used. As compared with batch cultivation the continuous production yields higher enzyme concentrations under optimal conditions and the fermentor productivity may be four to five times higher.
Similar content being viewed by others
References
Aunstrup I.C.: Production of industrial enzymes, p. 157 inBiotechnology and Fungal Differentiation (J. Meyrath, J.D. Bu’Lock, Eds). Academic Press, New York 1979.
Fencl Z., Pazlarová J., Placek J., Bgorova L.A., Loginova L.G.: Experimental verification of the results obtained by mathematical modelling of the production ofBacillus subtilis mutants synthesizing α-amylase at a low rate in the conditions of continuous cultivation.Mikrobiologyia 48, 93 (1979).
Fencl Z., Řičica J., Kodešová J.: The use of the multistage chemostat for microbial product formation.J. Appl. Chem. Biotechnol. 22. 405 (1972).
Hitotsuyanagi K., Yamagitchi K., Maruo B.: Stepwise induction of regulatory genes stimulating production of α-amylase intoBacillus subtilis: construction of an α-amylase extrahyper-producing strain.Agr. Biol. Chem. 43. 2343 (1979).
Imanaka T., Aiba S.: A kinetiv model of catabolite repression in the dual control mechanism in microorganisms.Biotechnol. Bioeng. 19, 757 (1977).
Imanaka I.: Optimization of microbial enzyme production-reactor modelling and optimization for production of α-galaetosidase by aMonascus sp., p. 399 inAnnual Reports of International Center of Cooperation Research and Development in Microbial Engineering (H. Taguchi, Ed.). Faculty of Engineering, Osaka 1979.
Mian F.A., Fencl Z., Prokop A.: Growth rate and enzyme activity in yeastCandida utilis, p. 105 inContinuous Cultivation of Microorganisms (I. Málek, K. Beran, Z. Fencl, V. Munk, J. Řičica, H. Smrčková,-Eds). Academia, Prague 1969.
Mikulik K., Jiránová A., Janda I., Weiser J.: Translation of poly(U) inStreptomyces aureofaciens producing tetracycline.Biochim. Biophys. Acta, in press (1982).
Pazlarová J., Fencl Z., Tsaplina I.A., Egorova L.A., Loginova L.G.: Production of amylase by periodic and continuous cultures ofBacillus subtilis.Mikrobiologiya 46, 450 (1977).
Pazlarová J., Ygorova L.A., Fencl Z., Loginova L.G.: Comparison of growth rates of two clones ofBacillus subtilis, producer of α-amylase.Folia Microbiol. 27, 323 (1982).
Pavlasová E., Stejskalová E., Novotný Č., Sikyta B.: Simultaneous synthesis of catabolic enzymes inEscherichia coli, p. 777 inContinuous Cultivation of Microorganisms (B. Sikyta, Z. Fencl, V. Poláček, Eds). Institute of Microbiology, Czechoslovak Academy of Sciences, Prague 1980.
Strome S., Young E.T.: Translational control of the expression of bacteriophage T7 gene 0.3.J. Mol. Biol. 125, 75 (1978).
Suga K.: Production of cellulase in fedbatch system, p. 397 inAnnual Reports of International Center of Cooperation Research and Development in Microbial Engineering (H. Taguchi, Ed.). Faculty of Engineering, Osaka 1979.
Terui G., Okada H., Kinoshita S.: Kinetic studies on enzyme production by microbes. (1). On kinetic models.J. Ferm. Technol. 45, 497 (1967).
Tichý P., Pazlarová J., Hartmann M., Fencl Z., Erbenová L., Benada O., Krumphanzl V.: Isolation of the pMI 10 plasmid from the α-amylase producing strain ofBacillus subtilis.Molec. Gen. Genet. 181, 248 (1981).
Toda K.: Dual control of invertase biosynthesis in chemostat culture.Biotechnol. Bioeng. 18, 1117 (1976).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fencl, Z., Pazlarová, J. Production of extracellular enzymes in continuous culture. Folia Microbiol 27, 340–349 (1982). https://doi.org/10.1007/BF02883137
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02883137