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Determination of sulfonamides with fluorescent inclusion complex of carbonic anhydrase and dansylamide

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Chinese Science Bulletin

Abstract

A new fluorimetric method for determination of sulfonamides was described based on the formation of a fluorescent inclusion complex of carbonic anhydrase(CAI with dansylamide(DNSA). The binding of DNSA to CA resulted in an enhancement in the fluorescence emission at 460 nrn with excitation at 280 nrn. Dissociation constants were determined for the carbonic anhydrase-sulfonamide complexes. Linear calibration graphs of sulfonamides were obtained within a concentration range of 0–0.058 μg/mL for DNSA; 0–0.344 pg/mL for sulfanilamide (SAN) and 0–0.286 μg/mL forP-toluene-sulfonamide (PTSN). The relative standard deviations were within 1.8%-4.2%. Limits of detection for DNSA, SAN and PTSN were 0.84, 19. 5 and 6.1 ng/mL, respectively. The method was applied to the determination of sulfonamides in cow milk at ng/mL level.

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References

  1. Salinas, F., Mansilla, A. E., Nevado, B., Derivative spectrophotometric determination of sulphonamides by the Brutton-Marshall reaction,Anal. Chim. Acta., 1990, 233: 289.

    Article  CAS  Google Scholar 

  2. Al-Abachi, M. Q., Salih, E. S., Salem, M. S., Application of promethazine hydrochloride as a chromogenic reagent for the spectrophotometric determination of certain sulfonamide drugs.Fresenius’ Z. Anal. Chem., 1990. 337: 408.

    Article  CAS  Google Scholar 

  3. Matusik, J. E., Sternal, R. S., Barnes, C. J.et al., Confirmation of identity by gas chromatography/ tandem mass spectrometry of sulfathiile, sulfamethazine, sulfachloropyrdazine, and sulfadimethoxine from bovine or swine liver extracts after quantitation by gas chromatography electron-capture detection,J. Assoc. Off. Anal. Chmr., 1990, 73(4): 529.

    CAS  Google Scholar 

  4. Smedley, M. D., Weber, J. D., Liquid chromatographic determination of multiple sulfonamide residues in bovine milk,J. Assoc. Off. Anal. Chem., 1990, 73(6): 875.

    PubMed  CAS  Google Scholar 

  5. Abian, J., Churchwell, M. I., Korfmacher, W. A., High-performance liquid chromatographythermospray mass spectrometry of ten sulfonamide antibiotics analysis in milk at ppb level,J. Chromatogr., 1993. 629: 267.

    Article  PubMed  CAS  Google Scholar 

  6. Patel, B. M., Moye, H. A., Weinberger, R., Postcolumn formation of fluoruphores from nitrogenous pesticides by UV photolysis,Talanta, 1991, 38: 913.

    Article  CAS  Google Scholar 

  7. Mahedero, M. C., Aaron, J. J., Flow-injection determination of sulphonamides with fluorimetric or photochemical-fluorimetric detection,Anal. Chim. Acta, 1992, 269: 193.

    Article  CAS  Google Scholar 

  8. Chen, R. F., Kernohan, J. C., Combination of bovine carbonic anhydrase with a fluorescent sulfonamide,J. Bio. Chem., 1967, 242: 5813.

    CAS  Google Scholar 

  9. Fersht, A.,Enzyme Structure and Mechanism, 2nd., W. H. Freeman and Company (1985) (translated by Du Jinzhu, Ru Binggen and Wei Xinching), Beijing: Peking University, 1991, 204.

    Google Scholar 

  10. Hernandez, F. H., Escriche, J. M., Fluorimetric determination of aluminium with morin after extraction with isobutyl methyl ketone, Part I. Fluorescence of the aluminium-morin complex in an isobutyl methyl ketone-ethanol-water system,Analyst., 1984, 109: 1585.

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, Peking University, 100871, Beijing, China

    Huaina Li & Yunxiang Ci

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  1. Huaina Li
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  2. Yunxiang Ci
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Li, H., Ci, Y. Determination of sulfonamides with fluorescent inclusion complex of carbonic anhydrase and dansylamide. Chin.Sci.Bull. 43, 1085–1090 (1998). https://doi.org/10.1007/BF02883078

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  • DOI: https://doi.org/10.1007/BF02883078

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