Abstract
The effect of 18 amino acids and 7 organic acids on the production ofl-asparaginase EC-2 by a strain ofEscherichia coli in a chemically defined medium was investigated under moderate aeration. All the amino acids and some of the organic acids stimulated the enzyme production. The specific activity without stimulants was about 0.16 nkat per mg dry weight, with stimulants it lay between 1 and 6 nkat per mg dry weight but withl-leucine andl-methionine the values were 12 nkat and 17 nkat per mg, respectively. When two organic or amino acids were added simultaneously at concentrations that were suboptimal for stimulation, the stimulating effects were cumulative in most cases. When cells were grown under conditions approaching anaerobiosis, the specific activity reached, even in the absence of stimulants, values as high as 5 nkat per mg; under these conditions, a further substantial increase in specific activity was only caused byl-leucine andl-methionine. Stimulating effects ofdl-lactate and of some amino acids were also found in other strains ofEscherichia coli. The ability to grow on a medium withl-asparagine as the sole source of both nitrogen and carbon was found in two strains; growth took place even when there was no measurable activity ofl-asparaginase EC-2.
Similar content being viewed by others
References
Bilimoria M. H.: Conditions for the production ofl-asparaginase 2 by coliform bacteria.Appl. Microbiol 18, 1025 (1969).
Boeck L. D., Squires E. W., Wilson M. W., Ho P. K.: Effect of glucose and low oxygen tension onl-asparaginase production by a strain ofEscherichia coli B.Appl. Microbiol. 20, 964 (1970).
Cedar H. H., Schwartz J. H.: Production ofl-asparaginase II byEscherichia coli.J. Bacteriol. 96, 2043 (1968).
Heinemann B., Howard A. J.: Production of tumor-inhibitoryl-asparaginase by submerged growth ofSerratia marcescens.Appl. Microbiol. 18, 550 (1969).
Heinemann B., Howard A. J., Palocz H. J.: Influence of dissolved oxygen levels on production ofl-asparaginase and prodigiosin bySerratia marcescens.Appl. Microbiol. 19, 800 (1970).
Liu F. S., Zajic J. E.:l-Asparaginase synthesis byErwinia aroideae.Appl. Microbiol. 23, 667 (1972).
Mardashev S. R., Yeremenko V. V., Nikolaev A. Ya.: Identification ofPseudomonas sp. and the effect of growth conditions on asparaginase and glutaminase activity. (In Russian)Mikrobiologiya 34, 11 (1970).
Nakamura N., Morikawa Y., Fujio T., Tanaka M.:l-Asparaginase fromEscherichia coli. Part I. Crystallization and properties.Agr. Biol. Chem. 35, 219 (1971).
Nakamura N., Fujio T., Tanaka M.: On the productivity and properties ofl-asparaginase fromEscherichia coli A-1-3.Agr. Biol. Chem. 36, 2251 (1972).
Netrval J.: Effect of organic and amino acids onl-asparaginase production byEscherichia coli.Arch. Microbiol. 92, 345 (1973).
Nikolayev A. Ya., Sokolov N. N., Mardashev S. R.: On the mechanism of induction of asparaginase and glutaminaae synthesis by aspartic and glutamic acids inPseudomonas fluorescens AG. (In Russian)Biokhimiya 36, 643 (1971).
Peterson R. E., Ciegler A.: Factors influencingl-asparaginase production byErwinia aroideae.Appl. Microbiol. 23, 671 (1972).
Roberts J., Burson G., Hill J. M.: New procedures for purification ofl-asparaginase with high yield fromEscherichia coli.J. Bacteriol. 95, 2117 (1968).
Robison R. S., Berk B.:l-Asparaginase synthesis byEscherichia coli B.Biotech. Bioeng. 11, 1211 (1969).
Svobodová O., ŠtrbBÁŇOVÁ-NEČINOVÁ S.: Induction ofl-asparaginase synthesis inEscherichia coli. Biochim.Biophys. Acta 321, 643 (1973).
Tohan Y. T., Asano J., Kobayashi M.: Study ofl-asparaginase production byRhodopseudomonas capsulatus. (I) A model for the induction ofl-asparaginase.J. Ferment. Technol. 49, 733 (1971).
Tosa T., Sano R., Yamamoto K., Nakamura M., Ando K., Chibata I.:l-Asparaginase fromProteus vulgaris.Appl. Microbiol. 22, 387 (1971).
Willis R. C., Woolfolk C. A.: Asparagine utihzation inEscherichia coli.J. Bacteriol. 118, 231 (1974).
Yeremenko V. V., Yevseyev L. P., Nikolayev A. Ya.: Asparaginase ofBacterium cadaveris. (In Russian)Mikrobiologiya 37, 213 (1968).
Yevseyev L. P., Nikolayev A. Ya.: Induced formation of asparagine and glutamine aynthetases byPseudomonas sp. (In Russian)Biokhimiya 33, 1106 (1968). $
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Netrval, J. Stimulation of L-asparaginase production inEscherichia coli by organic and amino acids. Folia Microbiol 22, 106–116 (1977). https://doi.org/10.1007/BF02881635
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02881635