Abstract
The aim of this work is the evaluation of tetraploid potato clones with germplasm fromS. chacoense andS. phureja for chipping and resistance to tuber soft rot (Erwinia carotovora subsp.carotovora). Tetraploid potato clones were obtained via 2n eggs through 2x–4x crosses. Cytological mechanisms were investigated in the diploid hybrid 2n-egg producer used as parent in the unilateral sexual polyploidization scheme; the omission of the second meiotic division and cytokinesis failure at the end of meiosis were identified with a genetic consequence equivalent to second division restitution (SDR). Tuber marketable yield, specific gravity and chip-processing ability evaluated at harvest and after cold storage with and without reconditioning were higher in some clones as compared to theS. tuberosum tetraploid parent. Tuber soft rot resistance, contributed byS. chacoense, was successfully transferred to the4x level. After inoculation withErwinia carotovora subsp.carotovora and estimation of the rotted area, one tetraploid clone proved to be resistant and three partially resistant. The genetic value of exotic germplasm and sexual polyploidization strategy are discussed in relation to potato improvement.
Resumen
El objetivo de este trabajo es la evaluatión de clones tetraploides de papa con germoplasma deS. chacoense yS. phureja para hojuelas y para resistencia a la pudrición blanda del tubérculo (Erwinia carotovora subsp.carotovora). Los clones tetraploides fueron obtenidos mediante cruces 2x–4x, utilizando gametos 2n. Los mecanismos citológicos fueron investigados en el productor diploide del híbrido del gameto2n usado como padre en el esquema sexual unilateral de poliploidización; la omisión de la segunda división meiótica y la falla de citocinesis al final de la meiosis fueron identificadas con una consecuencia genética equivalente a la segunda division de restitución (SDR).
Los tubérculos con rendimiento comercial, la gravedad específica y la habilidad para el procesamiento de hojuelas evaluados al momento de la cosecha y después del almacenaje en frío con y sin reacondicionamiento, fueron altos en algunos clones comparados con el padre tetraploide deS. tuberosum. La resistencia a la pudrición blanda del tubérculo, aportada porS. chacoense, fue transferida exitosamente al nivel4x. Después de la inoculación conErwinia carotovora subsp.carotovora y la estimación del área podrida, un clon tetraploide probó ser resistente y otros tres, parcialmente resistentes. El valor genético del germoplasma exótico y la estrategia de poliploidización sexual son discutidas en relación al mejoramiento de papa.
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Capo, A., Cammareri, M., Rocca, F.D. et al. Evaluation for chipping and tuber soft rot (Erwinia carotovora) resistance in potato clones from unilateral sexual polyploidization (2x × 4x). Am. J. Pot Res 79, 139–145 (2002). https://doi.org/10.1007/BF02881523
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DOI: https://doi.org/10.1007/BF02881523