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Histochemical studies of alpha and beta-glucosidases in the germinating pollen grains ofPortulaca grandiflora

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Biologia Plantarum

Abstract

The paper deals with the distribution of alpha and beta-glucosidases in the germinating pollen grains ofPortulaca grandiflora. Both these enzymes are localized in the pollen wall and the cytoplasmic granules. The latter are distributed throughout the pollen cytoplasm, pollen tube and stigma hair. In non-germinating pollen grains, enzymes are concentrated in the pollen wall. Stigma hair sheaths are completely free from enzymes. The functional significance of these enzymes in the hydrolysis of phenolic glycosides and polysaccharides is discussed.

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References

  • Ashfobd, A. E.: Histochemical localization of β-glucosidase inZea mays. 1. A simultaneous coupling azo dye technique for localization of β-glucosidase and β-galactosidase. - Protoplasma71: 281–293, 1970.

    Article  Google Scholar 

  • Ashfobd, A. E., McCully, M. E.: Histochemical localization of β-glucosidases in the roots ofZea mays. II. Changes in the localization and activity of β-glucosidase in the main root apex. - Protoplasma71: 389–402, 1970.

    Article  Google Scholar 

  • Brewbaker, J. L., Emery, G. C.: Pollen radiobotany. - Radiat. Bot.1: 101–154, 1962.

    Article  Google Scholar 

  • Den Tandt, W., Van Hoof, F.; Proc. 6th Meet. Fed. Europe Biochem. Soc. Abstract No. 838, 1969. - Cited in:Hers, H. G. andVan Hoof, F. (1973).

  • Hers, H. G.: α-Glucosidase deficiency in generalized glycogen-storage disease (Pompe’s disease). - Biochem. J.86: 11–16, 1963.

    PubMed  CAS  Google Scholar 

  • Hers, H. G., Van Hoof, F.: Lysosomes and Storage Diseases. - Acad. Press, New York-San Francisco-London 1973.

    Google Scholar 

  • Knox, R. B., Heslop-Harrison, J.: Pollen-wall proteins: localization and enzymes activity. - J. Cell Sci.6: 1–27, 1979.

    Google Scholar 

  • Malik, C. P., Tewabi, H. B., Sood, P. P.: On the functional significance of certain phosphatases in the germinating pollen grains ofPortulaca grandiflora. - Portug. Acta biol.11: 245 to 252, 1969.

    Google Scholar 

  • Martin, F. W.: Compounds from the stigma of 10 species. - Amer. J. Bot.56: 1023–1027, 1969.

    Article  CAS  Google Scholar 

  • Masoarenhas, J. P., Bell, E.: Protein synthesis during germination of pollen studies on polyribosome formation. -Biochim. biophys. Acta.179: 199–203, 1969.

    Google Scholar 

  • Pearse, A. G. E.: Histochemistry — Theoretical and Applied. - J. A. Churchill Publ. London 1961.

    Google Scholar 

  • Sood, P. P.: Histochemical studies on the distribution of non-specific esterase in the germinating pollen grains ofPortulaca grandiflora. - Biol. Plant.16: 102–106, 1974.

    CAS  Google Scholar 

  • Sood, P. P.: On the distribution and functional significance of β-galactosidase in the germinating pollen grains ofPortulaca grandiflora. - Biologica8: 72–80, 1975.

    Google Scholar 

  • Sood, P. P.: Histoenzymological compartmentation of β-glucuronidase in the germinating pollen grains ofPortulaca grandiflora. - Biol. Plant.22: 124–127, 1980.

    CAS  Google Scholar 

  • Sood, P. P., Malik, C. P., Tewari, H. B.: Histochemical studies on the localization of succinic dehydrogenase in the germinating pollen grains ofPortulaca grandiflora. - Z. Biol.116: 215 to 220, 1969a.

    Google Scholar 

  • Sood, P. P., Tewari, H. B., Malik, C. P.: Histochemical studies on the distribution of monoamine oxidase in the germinating pollen grains ofPortulaca grandiflora. - Beitr. Biol. Pflanzen46: 239–245, 1969b.

    Google Scholar 

  • Stanley, R. G., Linskens, H.: Pollen Biology — Biochemistry, Management. - Springer-Verlag, Berlin-Heidelberg-New York 1974.

    Google Scholar 

  • Tewari, H. B., Malik, C. P., Sood, P. P.: Histochemical studies on the distribution of 5-nucleotidase in the germinating pollen grains ofEschscholtzia californica Cham. - Biol. Plant.14: 269–272, 1972.

    Article  CAS  Google Scholar 

  • Vaes, G.: Digestive capacity of lysosomes. - In:Hers, H. G., Van Hoof, F. (ed.): Lysosomes and Storage Diseases. Pp. 43–77. Acad. Press, Now York-San Francisco-London 1973.

    Google Scholar 

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Sood, P.P., Chauhan, Z.T. Histochemical studies of alpha and beta-glucosidases in the germinating pollen grains ofPortulaca grandiflora . Biol Plant 24, 407–409 (1982). https://doi.org/10.1007/BF02880434

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  • DOI: https://doi.org/10.1007/BF02880434

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