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Histochemical localization of β-glycosidases in roots ofZea mays

I. a simultaneous coupling azo-dye technique for the localization of β-glucosidase and β-galactosidase

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Summary

A simultaneous coupling azo dye technique using 6-bromo-2-naphthylβ-D-glucoside as substrate has been developed for the localization ofβ-glucosidase in hand sections of plant root tissue. This technique is also applicable toβ-galactosidase localization. Using corn root hairs and epidermal cells as an experimental system, the localization obtained in simultaneous coupling has been compared with that obtained in the older post-coupling method, and in various control sections. It is concluded that the localization obtained in root hairs in the post-coupling method is an artifact produced by diffusion of the product before coupling to substantive non-enzymatic sites in the cytoplasm.

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Author notes

  1. Anne E. Ashford

    Present address: Department of Developmental Biology, Research School of Biological Sciences, Australian National University, Canberra, Australia

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  1. Department of Biology, Carleton University, Ottawa 1, Canada

    Anne E. Ashford

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  1. Anne E. Ashford
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Ashford, A.E. Histochemical localization of β-glycosidases in roots ofZea mays . Protoplasma 71, 281–293 (1970). https://doi.org/10.1007/BF01279637

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  • DOI: https://doi.org/10.1007/BF01279637

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