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Comparative analysis of the pig BAC sequence involved in the regulation of myostatin gene

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Abstract

Myostatin (GDF8, MSTN) is a member of the transforming growth factor beta superfamily that is essential for proper regulation of skeletal muscle mass. In order to study its expression and regulatory mechanism deeply, we have presented a comparative analysis of about 170-kb pig BAC sequence containing the myostatin gene among pig, human and mouse. The genomic region is characterized by high interspersed repeats and low G+C content. As for the myostatin gene, a higher sequence similarity is found between human and pig than between these species and the mouse. One striking feature is that the structure of two TATA-boxes in the nearby downstream of CCAAT-box is identified in the promoter. Further analysis reveals that the TATA-box1 is responsible for the transcription in pig and human, but the TATA-box2 acts on the transcription in mouse. The other interesting feature is that two polyadenylation signal sequences (AATAAA) exist in 3’UTR of the pig myostatin gene. Moreover, a large number of potential transcription factor-binding sites are also identified in evolutionary conserved regions (ECRs), which may be associated with the regulation of myostatin. Many putative transcription factors play an important role in the muscle development, and the complex interaction between myostatin and these factors may be required for proper muscle development.

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Authors and Affiliations

  1. State Key Laboratory for Agrobiotechnology, China Agricultural University, 100094, Beijing, China

    Zhengquan Yu, Qingyong Meng, Jing Yuan, Zhihui Zhao, Xiaoxiang Hu, Bingxue Yan, Baoliang Fan, Shuyang Yu & Ning Li

  2. Beijing Genomics Institute / Genomics and Bioinformatics Center, Institute of Genetics and Development Biology, Chinese Academy of Sciences, 100101, Beijing, China

    Yan Li & Wei Li

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  1. Zhengquan Yu
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Correspondence to Ning Li.

Additional information

The sequence data described in this paper have been submitted to GenBank under accession No. AY208121.

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Yu, Z., Li, Y., Meng, Q. et al. Comparative analysis of the pig BAC sequence involved in the regulation of myostatin gene. Sci. China Ser. C.-Life Sci. 48, 168–180 (2005). https://doi.org/10.1007/BF02879670

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  • DOI: https://doi.org/10.1007/BF02879670

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