Abstract
The effect of the proteins extracted with 1 M LiCl from theCicer arietinum etiolated epicotyl cell walls on the degradation of polysaccharides extracted with alkali was studied. The hemicellulosic polysaccharides were fractionated into three fractions extracted with 4 % KOH, 4 % KOH containing 8 M urea, and 24 % KOH. The protein extract showed exo-glycanase activity against all three hemicellulosic fractions whilst endo-glycanase activity was shown mainly against the hemicellulosic polysaccharides extracted with 4% KOH.
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This work was partially supported by a grant from DGICYT (PB89-554, MEC, Spain). We wish to thank Drs G. Revilla and E.P. Lorences for critical reading the manuscript and to Mr D.A. Lindsay for correcting the English version.
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Acebes, J.L., Zarra, I. Glycanase activities associated with cell walls ofCicer arietinum L. epicotyls. Biol Plant 38, 39–46 (1996). https://doi.org/10.1007/BF02879631
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DOI: https://doi.org/10.1007/BF02879631