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Study of rat neuronal genes with ordered differential display method

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Abstract

Ordered differential display (ODD) was developed recently and has been applied to systematic comparison of expression profiles of genes. It was further improved with the specific complexing property between biotin and streptavidin by the authors. First, random primer and biotinylated oligo (dT) primer were used to make pools of double strand cDNA. Second, streptavidin-coated PCR tube is used to absorb 3′ESTs specifically to avoid the negative effect of other DNA fragments. In the case of 3′ESTs comparison patterns between embryonic brain and body of SD rat, more than forty differentially expressed genes were cloned and identified. The function ofrZIC gene, one of the genes identified and cloned, was studied through ethological experiments. The result showed thatrZIC gene was associated with locomotion activity of adult mice.

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Authors and Affiliations

  1. Shanghai Research Center of Biotechnology, Institutes for Biological Sciences, Chinese Academy of Sciences, 200233, Shanghai, China

    Jiansheng Kang, Zhiping Wang, Meilei Jin, Ning Guo & Rongxiu Li

  2. Shanghai Institute of Biochemistry and Cell Biology, Institutes for Biological Sciences, Chinese Academy of Sciences, 200031, Shanghai, China

    Jiansheng Kang & Yucang Du

  3. Shanghai Research Center of Transgenic Research, 201300, Shanghai, China

    Ting Wang

Authors
  1. Jiansheng Kang
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  2. Zhiping Wang
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  3. Meilei Jin
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  4. Ning Guo
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  5. Ting Wang
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  6. Yucang Du
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  7. Rongxiu Li
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Correspondence to Rongxiu Li.

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Kang, J., Wang, Z., Jin, M. et al. Study of rat neuronal genes with ordered differential display method. Sci. China Ser. C.-Life Sci. 44, 601–609 (2001). https://doi.org/10.1007/BF02879354

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  • DOI: https://doi.org/10.1007/BF02879354

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