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Recording of calcium transient and analysis of calcium removal mechanisms in cardiac myocytes from rats and ground squirrels

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Abstract

With confocal microscopy, we recorded calcium transients and analyzed calcium removal rate at different temperatures in cardiac myocytes from the rat, a non-hibernator, and the ground squirrel, a hibernator. The results showed a remarkable increase of the diastolic level of calcium transients in the rat but no detectable change in the ground squirrel. Calcium transient of the ground squirrel, compared with that of the rat at the same temperature, had a shorter duration and showed a faster calcium removal. As indicated by the pharmacological effect of cyclopiazonic acid, calcium uptake by sarcoplasmic reticulum (SR) was the major mechanism of calcium removal, and was faster in the ground squirrel than in the rat. Our results confirmed the essential role of SR in hypothermia-tolerant adaptation, and negated the importance of Na-Ca exchange. We postulated the possibility to improve hypothermia-tolerance of the cardiac tissue of non-hibernating mammals.

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References

  1. Wang, S. Q., Huang, Y. H., Zhou, Z. Q., Dependence of myocardial hypothermia resistance on sources of activator calcium, Cryobiology, 1997, 35: 193.

    Article  PubMed  CAS  Google Scholar 

  2. Liu, B., Wohlfart, B., Johansson, B. W., Effects of low temperature on contraction in papillary muscles from rabbit, rat and hedgehog, Cryobiology, 1990, 27: 539.

    Article  PubMed  CAS  Google Scholar 

  3. Wang, S. Q., Zhou, Z. Q., Qian, H., Temperature dependence of intracellular free calcium in cardiac myocytes from rat and ground squirrel measured by confocal microscopy, Science in China, Ser. C, 1999, 42(3): 293.

    Article  CAS  PubMed  Google Scholar 

  4. Liu, B., Belke, D. D., Wang, L. C. H., Ca2+ uptake by cardiac sarcoplasmic reticulun at low temperature in rat and ground squirrel, Am. J. Physiol., 1997, 272: R1121.

    PubMed  CAS  Google Scholar 

  5. Belke, D. D., Milner, R. E., Wang, L. C. H., Seasonal variations in the rate and capacity of cardiac SR calcium accumulation in a hibernating species, Cryobiology, 1991, 28: 354.

    Article  PubMed  CAS  Google Scholar 

  6. Tang, Y. J., Wang, S. Q., Zhou, Z. Q., Seasonal variation in the ultrastructure and calcium uptake rate of cardiac sarcoplasmic reticulum in ground squirrel, Acta Physiologica Sinica (in Chinese), 1995, 47: 478.

    CAS  Google Scholar 

  7. Kondo, N., Electrophysiological effects of Ca antagonists, tetrodotoxin, [Ca]0 and [Na]0 on myocardium of hibernating chipmunks: possible involvement of Na-Ca exchange mechanism, Br. J. Pharmacol., 1987, 91: 315.

    PubMed  CAS  Google Scholar 

  8. Smith, T. W., Yamaoka, K., Twist, V. W. et al., Membrane transport in isolated cardiac myocytes of ground squirrels: cold sensitivity of pumps and channels, in Living in the Cold II (eds. Malan, A., Canguilhem, B.), London: John Libbey Eurotext, 1989, 177.

    Google Scholar 

  9. Grynkiewicz, G., Poenie, M., Tien, R. Y., A new generation of Ca2+ indicators with greatly improved fluorescent properties, J. Biol. Chem., 1985, 260: 3440.

    PubMed  CAS  Google Scholar 

  10. Wang, S. Q., Zhou, Z. Q., Alpha-stat calibration of indo-1 fluorescence and measurement of intracellular free calcium in rat heart cells at different temperatures, Life Sciences, 1999, 65(9): 871.

    Article  PubMed  CAS  Google Scholar 

  11. Balke, C. W., Egan, T. M., Wier, W. G., Processes that remove calcium from the cytoplasm during excitation-contraction coupling in intact rat heart cells, J. Physiol. (London), 1994, 474: 447.

    CAS  Google Scholar 

  12. Puglisi, J. L., Bassani, R. A., Bassani, J. W. et al., Temperature and relative contributions of Ca transport systems in cardiac myocyte, Am. J. Physiol., 1996, 270: H1772.

    PubMed  CAS  Google Scholar 

  13. Zhang, Y., Xue, S., Calcium and cell function, in Progress in Cellular Biology (eds. Zheng, G., Zhai, Z.) (in Chinese), Vol. 2, Beijing: Higher Education Press, 1991, 160.

    Google Scholar 

  14. Marengo, F. D., Wang, S. Y., Langer, G. A., The effects of temperature upon calcium exchange in intact cultured cardiac myocytes, Cell Calcium, 1997, 21: 263.

    Article  PubMed  CAS  Google Scholar 

  15. Wang, S. Q., Zhou, Z. Q., Gao, T. L., Features of the cardiovascular function in mammalian hibernators, Acta Zoologica Sinica (in Chinese), 1997, 42: 203.

    Google Scholar 

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Authors and Affiliations

  1. College of Life Sciences, Peking University, 100871, Beijing, China

    Shiqiang Wang & Zengquan Zhou

  2. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081, Beijing, China

    Hong Qian

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  1. Shiqiang Wang
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  2. Zengquan Zhou
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  3. Hong Qian
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Correspondence to Shiqiang Wang.

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Wang, S., Zhou, Z. & Qian, H. Recording of calcium transient and analysis of calcium removal mechanisms in cardiac myocytes from rats and ground squirrels. Sci. China Ser. C.-Life Sci. 43, 191–199 (2000). https://doi.org/10.1007/BF02879128

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  • DOI: https://doi.org/10.1007/BF02879128

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