Abstract
Structural and molecular changes in cardiomyocytes of hibernating ground squirrels in different periods of the annual cycle were analyzed by means of electron microscopy and polymerase chain reaction. Morphological analysis showed an increase in relative area of sarcoplasmic reticulum in cardiac muscle of ground squirrels preparing to torpor compared to active summer animals. The size of sarcoplasmic reticulum in cardiomyocytes of torpid animals was reliably less than in any other condition of ground squirrels in the annual cycle. The results of molecular analysis showed the decrease in sarcoplasmic reticulum Ca2+-ATPase gene (SERCA2a) expression at all stages of hibernation process and also in periods of autumn activity compared to control mRNA level in active summer animals. The revealed season changes in structure of sarcoplasmic reticulum and sarcoplasmic reticulum Ca2+-ATPase gene expression are discussed in regard to adaptation of ground squirrels to hibernation.
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References
G. D. Duker, S. O. Olsson, N. H. Hecht, et al., Cryobiol. 20(4), 407 (1983).
B. W. Johansson, Card. Res. 3(I), 826 (1996).
A. Mattu, W. J. Brady, and A. D. Perron, Am. J. Emerg. Med. 20(4), 314 (2002).
F. van Breukelen and S. L. Martin, Molecular, J. Appl. Physiol. 92, 2640 (2002).
S. Q. Wang, E. G. Lakatta, H. Cheng, et al., J. Exp. Biol. 205(19), 2957 (2002).
D. D. Belke, R. E Milner, and L. C. H. Wang, Cryobiol. 28, 354 (1991).
B. Liu, D. D. Belke, and L. C. H. Wang, Am. J. Physiol. 272, R1121 (1997).
A. M. Rubtsov, Ros. Fiziol. Zh. im. Sechenova 91(2), 141 (2005).
S. Giroud, C. Frare, A. Strijkstra, et al., PLoS ONE 8(5), e63111 (2013).
N. M. Zakharova, O. V. Nakipova, A. S. Averin, et al., Dokl. RAN 424(5), 1 (2009).
E. V. Karaduleva, I. M. Vikhlyantsev, M. N. Tutukina, et al., Vestn. Biotekh. Fiziko-khim. Biol. im. Ovcinnikova 6(4), 5 (2010).
N. M. Zakharova, Fund. Issl. 6(7), 1401 (2014).
H. V. Carey, M. T. Andrews, and S. L. Martin, Physiol. Rev. 83, 1153 (2003).
I. M. Santalova, N. M. Zakharova, R. N. Khramov, et al., Biofizika 53(5), 879 (2008).
E. V. Karaduleva, I. M. Vikhlyantsev, and Z. A. Podlubnaya, Biofizika 55(4), 612 (2010).
K. J. Livak and T. D. Schmittgen, Methods 25(4), 402 (2001).
N. Kondo, Circ. Res. 59, 221 (1986).
T. H. Rosenquist, Cryobiol. 7, 14 (1970).
Y. J Tang, S. Q. Wang, and Z. Q. Zhou, Acta Physiol. Sinica 547, 478 (1995).
A. Yatani, S. J. Kim, R. K. Kudej, et al., Am. J. Physiol. Heart. Circ. Physiol. 286, H2219 (2004).
A. Ascensão, R. Ferreira, and J. Magalhães, International J. of Cardiology 117, 16 (2007).
O. Nakipova, N. Zakharova, L. Andreeva, et al., Cryobiology 55, 173 (2007).
J. Yan, B. M. Barnes, F. Kohl, et al., Physiol. Genomics. 32, 170 (2008).
K. B. Storey, in: Hypoxia: Through the Lifecycle, Adv. Exper. Medic. Biol. 543, 21 (2003).
L. E. Epperson and S. L. Martin, Genomics 10(2), 93 (2002).
K. M. Brauch, N. D. Dhruv, E. A. Hanse, et al., Physiol. Genomics. 23, 227 (2005).
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Original Russian Text © E.V. Karaduleva, I.M. Santalova, N.M. Zakharova, 2014, published in Biofizika, 2014, Vol. 59, No. 5, pp. 926–932.
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Karaduleva, E.V., Santalova, I.M. & Zakharova, N.M. Specific molecular and morphological changes in cardiomyocytes of hibernating ground squirrels in different periods of annual cycle. BIOPHYSICS 59, 752–757 (2014). https://doi.org/10.1134/S0006350914050091
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DOI: https://doi.org/10.1134/S0006350914050091