Abstract
An Arabidopsis cell death mutation locus was mapped to chromosome 2 between /GS1 and mi421. The YAC clone ends, CIC9A3R, CIC11C7L, CIC2G5R and RFLP marker CDs3 within this interval, were used to probe TAMU BAC library and 31 BAC clones were obtained. A BAC contig encompassing the mutation locus, which consists of T6P5, T7M23, T12A21, T8L6 and T18A18, was identified by Southern hybridization with the BAC ends as probes. 11 CAPS and 12 STS markers were developed in this region. These results will facilitate map-based cloning of the genes and sequencing of the genomic DNA in this region.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Meinke, D. W., Cherry, J. M., Dean, C. et al.,Arabidopsis thaliana: a model plant for genome analysis, Science, 1998, 282: 662
Dietrich, R. A., Delaney, T. P., Uknes, S. J. et al., Arabidopsis mutants simulating disease resistance response, Cell, 1994, 77: 565.
Heath, M. C., Apoptosis, programmed cell death and the hypersensitive response, Eur. J. Plant Pathol., 1998, 104: 117.
Mittler, R., Lam, E.,In situ detection of nDNA fragmentation during the differentiation of tracheary elements in higher plants, Plant Physiol., 1995, 108: 489.
Yeung, E. C., Meinke, D. W., Embryogenesis in angiosperms: Development of the suspensor, Plant Cell, 1993, 5: 1371.
Pennell, R. I., Lamb, C., Programmed cell death in plants, Plant Cell, 1997, 9: 1157.
Zachgo, E. A., Wang, M. L., Dewdney, J. et al., A physical map of chromosome 2 ofArabidopsis thaliana, Genome Research, 1996, 6: 19.
Creusot, F., Fouilloux, E., Dron, M. et al., The CIC library: a large insert YAC library for genome mapping in Arabidoposis, Plant J., 1995, 8: 763.
Choi, S., Creelman, R. A., Mullet, J. E. et al., Construction and characterization of a bacterial artificial chromosome library ofArabidopsis thaliana, Plant Mol. Biol. Report, 1995, 13: 124.
Konieczny, A., Ausubel, F. M., A procedure for mapping Arabidopsis mutations using codominant ecotype-specific PCR-based markers, Plant J., 1993, 3: 403.
Inoue, T., Zhong, H. S., Miyao, A. et al., Sequence-tagged sites (STSs) as standard landmarks in the rice genome, Theor. Appl. Genet., 1994, 89: 728.
Li, J., Zhao, J., Rose, A. B. et al., Arabidopsis phosphoribosylanthranilate isomerase: molecular genetic analysis of triplicate tryptophan pathway genes, Plant Cell, 1995, 7: 447.
Barczak, A. J., Zhao, J., Pruitt, K. D. et al., 5-Fluoroindole resistance identifies tryptophan synthase beta subunit mutants in Arabidopsis, Genetics, 1995, 140: 303.
Sambrook, J., Fritsch, E. F., Maniatis, T., Molecular Cloning: A Laboratory Manual, 2nd ed., New York: Cold Spring Harbor Laboratory Press, 1989.
Ausubel, F. M., Brent, R., Kingston, R. E. et al., Current Protocols in Molecular Biology, Vol. 2., New York: John Wiley and Sons Inc., 1997.
Gibson, S. I., Somerville, C. R., Chromosome walking inArabidopsis thaliana using yeast artificial chromosomes, in Methods in Arabidopsis Research (eds. Koncz, C., Chua, N. H., Schell, J.), Singapore: World Scientific, 1992, 119–143.
Liu, Y. G., Whittier, R. F., Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking, Genomics, 1995, 25: 674.
Harushima, Y., Yano, M., Shomura, A. et al., A high-density rice genetic linkage map with 2275 markers using a single F2 population, Genetics, 1998, 148: 479.
Bell, C. J., Ecker, J. R., Assignment of 30 microsatellite loci to the linkage map of Arabidopsis, Genetics, 1994, 19: 137.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Mou, Z., Dai, Y. & Li, J. Fine-mapping of an Arabidopsis cell death mutation locus. Sci. China Ser. C.-Life Sci. 43, 138–145 (2000). https://doi.org/10.1007/BF02879122
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02879122